Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh

Poor hygiene and limited food safety awareness among meat handlers contribute significantly to foodborne diseases in Bangladesh. This study assessed the microbiological quality of raw meat in slaughterhouses across Gopalganj district and evaluated the knowledge, attitudes, and practices (KAP) of mea...

Full description

Saved in:
Bibliographic Details
Main Authors: Nur Mohammed Imran Hossain, Md Entaduzzaman Jony, Progati Bakshi, Juwel Rana, Zannatul Ferdoush, Md Golam Maula Shomrat, Md Mehedi Hasan Titu, Daniel Chakma, Lima Khatun, Muha Rakibul Islam
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Journal of Agriculture and Food Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666154325002856
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850138075199438848
author Nur Mohammed Imran Hossain
Md Entaduzzaman Jony
Progati Bakshi
Juwel Rana
Zannatul Ferdoush
Md Golam Maula Shomrat
Md Mehedi Hasan Titu
Daniel Chakma
Lima Khatun
Muha Rakibul Islam
author_facet Nur Mohammed Imran Hossain
Md Entaduzzaman Jony
Progati Bakshi
Juwel Rana
Zannatul Ferdoush
Md Golam Maula Shomrat
Md Mehedi Hasan Titu
Daniel Chakma
Lima Khatun
Muha Rakibul Islam
author_sort Nur Mohammed Imran Hossain
collection DOAJ
description Poor hygiene and limited food safety awareness among meat handlers contribute significantly to foodborne diseases in Bangladesh. This study assessed the microbiological quality of raw meat in slaughterhouses across Gopalganj district and evaluated the knowledge, attitudes, and practices (KAP) of meat handlers. A cross-sectional survey of 60 meat handlers from 20 slaughterhouses was conducted between February and October 2024 using structured questionnaires, and 100 raw meat samples underwent microbiological analysis for Total Staphylococcus aureus Count (TSAC), Total Viable Count (TVC), and Total E. coli Count (TEC).Findings revealed that only 28.3 % of handlers had adequate food safety knowledge (mean score: 8.12 ± 3.03 out of 20). Positive attitudes were observed in 88.3 % (mean score: 46.19 ± 8.28 out of 75), but only 30 % exhibited good hygiene practices (mean score: 25.63 ± 8.9 out of 75). Education level was significantly associated with food safety practices (p = 0.021), while other demographic factors showed no significant associations with KAP. Significant positive correlations (p < 0.001) were found between knowledge, attitudes, and practices. Microbial analysis showed widespread contamination, with 93 % and 87 % of meat samples exceeding acceptable TEC and TSAC limits, respectively. Gopalganj Sadar recorded the highest TVC (7.76 log10 CFU/g), while Kashiani showed the highest TEC (4.56 log10 CFU/g) and TSAC (5.08 log10 CFU/g).These findings highlight the urgent need for slaughterhouse infrastructure improvements and targeted training programs to mitigate contamination risks and safeguard public health.
format Article
id doaj-art-873fe9a4173f40538de3a7f06005f7ef
institution OA Journals
issn 2666-1543
language English
publishDate 2025-06-01
publisher Elsevier
record_format Article
series Journal of Agriculture and Food Research
spelling doaj-art-873fe9a4173f40538de3a7f06005f7ef2025-08-20T02:30:39ZengElsevierJournal of Agriculture and Food Research2666-15432025-06-012110191410.1016/j.jafr.2025.101914Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, BangladeshNur Mohammed Imran Hossain0Md Entaduzzaman Jony1Progati Bakshi2Juwel Rana3Zannatul Ferdoush4Md Golam Maula Shomrat5Md Mehedi Hasan Titu6Daniel Chakma7Lima Khatun8Muha Rakibul Islam9Department of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Nutrition and Food Engineering, Daffodil International University, Dhaka-1216, Bangladesh; Corresponding author. Department of Nutrition &amp; Food Engineering, Daffodil International University, Daffodil Smart City, Birulia, Savar, Dhaka-1216, Bangladesh.Department of Nutrition and Food Engineering, Daffodil International University, Dhaka-1216, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshDepartment of Food Engineering, Gopalganj Science and Technology University, Gopalganj-8100, BangladeshPoor hygiene and limited food safety awareness among meat handlers contribute significantly to foodborne diseases in Bangladesh. This study assessed the microbiological quality of raw meat in slaughterhouses across Gopalganj district and evaluated the knowledge, attitudes, and practices (KAP) of meat handlers. A cross-sectional survey of 60 meat handlers from 20 slaughterhouses was conducted between February and October 2024 using structured questionnaires, and 100 raw meat samples underwent microbiological analysis for Total Staphylococcus aureus Count (TSAC), Total Viable Count (TVC), and Total E. coli Count (TEC).Findings revealed that only 28.3 % of handlers had adequate food safety knowledge (mean score: 8.12 ± 3.03 out of 20). Positive attitudes were observed in 88.3 % (mean score: 46.19 ± 8.28 out of 75), but only 30 % exhibited good hygiene practices (mean score: 25.63 ± 8.9 out of 75). Education level was significantly associated with food safety practices (p = 0.021), while other demographic factors showed no significant associations with KAP. Significant positive correlations (p < 0.001) were found between knowledge, attitudes, and practices. Microbial analysis showed widespread contamination, with 93 % and 87 % of meat samples exceeding acceptable TEC and TSAC limits, respectively. Gopalganj Sadar recorded the highest TVC (7.76 log10 CFU/g), while Kashiani showed the highest TEC (4.56 log10 CFU/g) and TSAC (5.08 log10 CFU/g).These findings highlight the urgent need for slaughterhouse infrastructure improvements and targeted training programs to mitigate contamination risks and safeguard public health.http://www.sciencedirect.com/science/article/pii/S2666154325002856Food safetyMeat handlersMicrobial contaminationHygiene practicesSlaughterhousesPublic health
spellingShingle Nur Mohammed Imran Hossain
Md Entaduzzaman Jony
Progati Bakshi
Juwel Rana
Zannatul Ferdoush
Md Golam Maula Shomrat
Md Mehedi Hasan Titu
Daniel Chakma
Lima Khatun
Muha Rakibul Islam
Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
Journal of Agriculture and Food Research
Food safety
Meat handlers
Microbial contamination
Hygiene practices
Slaughterhouses
Public health
title Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
title_full Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
title_fullStr Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
title_full_unstemmed Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
title_short Food safety knowledge, attitude, and hygiene practices (KAP) and microbial quality of meat in slaughterhouses: a study in gopalganj, Bangladesh
title_sort food safety knowledge attitude and hygiene practices kap and microbial quality of meat in slaughterhouses a study in gopalganj bangladesh
topic Food safety
Meat handlers
Microbial contamination
Hygiene practices
Slaughterhouses
Public health
url http://www.sciencedirect.com/science/article/pii/S2666154325002856
work_keys_str_mv AT nurmohammedimranhossain foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT mdentaduzzamanjony foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT progatibakshi foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT juwelrana foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT zannatulferdoush foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT mdgolammaulashomrat foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT mdmehedihasantitu foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT danielchakma foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT limakhatun foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh
AT muharakibulislam foodsafetyknowledgeattitudeandhygienepracticeskapandmicrobialqualityofmeatinslaughterhousesastudyingopalganjbangladesh