Zika structural genes determine the virulence of African and Asian lineages
The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together wi...
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| Format: | Article |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1753583 |
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| author | Bruno T. D. Nunes Camila R. Fontes-Garfias Chao Shan Antonio E. Muruato Jannyce G. C. Nunes Rommel M. R. Burbano Pedro F. C. Vasconcelos Pei-Yong Shi Daniele B. A. Medeiros |
| author_facet | Bruno T. D. Nunes Camila R. Fontes-Garfias Chao Shan Antonio E. Muruato Jannyce G. C. Nunes Rommel M. R. Burbano Pedro F. C. Vasconcelos Pei-Yong Shi Daniele B. A. Medeiros |
| author_sort | Bruno T. D. Nunes |
| collection | DOAJ |
| description | The Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together with our previously developed Asian ZIKV strain IC, allowed us to engineer chimeric viruses by swapping the structural and non-structural genes between the two lineages. Recombinant parental and chimeric viruses were analyzed in A129 and newborn CD1 mouse models. In the A129 mice, the African strain developed higher viremia, organ viral loading, and mortality rate. In CD1 mice, the African strain exhibited a higher neurovirulence than the Asian strain. A chimeric virus containing the structural genes from the African strain is more virulent than the Asian strain, whereas a chimeric virus containing the non-structural genes from the African strain exhibited a virulence comparable to the Asian strain. These results suggest that (i) African strain is more virulent than Asian strain and (ii) viral structural genes primarily determine the virulence difference between the two lineages in mouse models. Other factors may contribute to the discrepancy between the mouse and epidemic results. |
| format | Article |
| id | doaj-art-873cb846c462440a8b8b6ac3cde20c4a |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-873cb846c462440a8b8b6ac3cde20c4a2025-08-20T03:08:32ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-01911023103310.1080/22221751.2020.1753583Zika structural genes determine the virulence of African and Asian lineagesBruno T. D. Nunes0Camila R. Fontes-Garfias1Chao Shan2Antonio E. Muruato3Jannyce G. C. Nunes4Rommel M. R. Burbano5Pedro F. C. Vasconcelos6Pei-Yong Shi7Daniele B. A. Medeiros8Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, BrazilDepartment of Biochemistry & Molecular Biology, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, Galveston, TX, USADepartment of Biochemistry & Molecular Biology, Galveston, TX, USAHealth Sciences Institute, Belem, BrazilDepartment of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, BrazilDepartment of Biochemistry & Molecular Biology, Galveston, TX, USADepartment of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ministry of Health, Ananindeua, BrazilThe Asian lineage of Zika virus (ZIKV) is responsible for the recent epidemics in the Americas and severe disease, whereas the African lineage of ZIKV has not been reported to cause epidemics or severe disease. We constructed a cDNA infectious clone (IC) of an African ZIKV strain, which, together with our previously developed Asian ZIKV strain IC, allowed us to engineer chimeric viruses by swapping the structural and non-structural genes between the two lineages. Recombinant parental and chimeric viruses were analyzed in A129 and newborn CD1 mouse models. In the A129 mice, the African strain developed higher viremia, organ viral loading, and mortality rate. In CD1 mice, the African strain exhibited a higher neurovirulence than the Asian strain. A chimeric virus containing the structural genes from the African strain is more virulent than the Asian strain, whereas a chimeric virus containing the non-structural genes from the African strain exhibited a virulence comparable to the Asian strain. These results suggest that (i) African strain is more virulent than Asian strain and (ii) viral structural genes primarily determine the virulence difference between the two lineages in mouse models. Other factors may contribute to the discrepancy between the mouse and epidemic results.https://www.tandfonline.com/doi/10.1080/22221751.2020.1753583ZikavirulencelineagesAfricanAsian |
| spellingShingle | Bruno T. D. Nunes Camila R. Fontes-Garfias Chao Shan Antonio E. Muruato Jannyce G. C. Nunes Rommel M. R. Burbano Pedro F. C. Vasconcelos Pei-Yong Shi Daniele B. A. Medeiros Zika structural genes determine the virulence of African and Asian lineages Emerging Microbes and Infections Zika virulence lineages African Asian |
| title | Zika structural genes determine the virulence of African and Asian lineages |
| title_full | Zika structural genes determine the virulence of African and Asian lineages |
| title_fullStr | Zika structural genes determine the virulence of African and Asian lineages |
| title_full_unstemmed | Zika structural genes determine the virulence of African and Asian lineages |
| title_short | Zika structural genes determine the virulence of African and Asian lineages |
| title_sort | zika structural genes determine the virulence of african and asian lineages |
| topic | Zika virulence lineages African Asian |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1753583 |
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