Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer
The research endeavors to expound the role of ORM1 in bladder cancer (BCa) and the implied response mechanism. RT-qPCR and Western blotting examined ORM1 and S100A12 expression. Functional experiments assessed the cellular phenotypes. HDOCK and Co-IP confirmed the interaction of ORM1 and S100A12. We...
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Taylor & Francis Group
2025-12-01
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| Series: | Cell Adhesion & Migration |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19336918.2024.2434209 |
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| author | Zhe Liu Xiaofeng Pu |
| author_facet | Zhe Liu Xiaofeng Pu |
| author_sort | Zhe Liu |
| collection | DOAJ |
| description | The research endeavors to expound the role of ORM1 in bladder cancer (BCa) and the implied response mechanism. RT-qPCR and Western blotting examined ORM1 and S100A12 expression. Functional experiments assessed the cellular phenotypes. HDOCK and Co-IP confirmed the interaction of ORM1 and S100A12. Western blotting tested apoptosis- and ERK signaling-associated proteins. ORM1 and S100A12 were abundant in the BCa cells. ORM1 or S100A12 loss impaired cell proliferation, migration, and invasion, and aggravated cell apoptosis. ORM1 interacted with S100A12. ORM1 knockdown down-regulated S100A12 expression and inactivated ERK signaling.S100A12 overexpression or ERK activator reversed the impacts of ORM1 interference on ERK signaling and BCa cells. ORM1 mightdrive BCa via binding to S100A12 and activating ERK signaling. |
| format | Article |
| id | doaj-art-87378046d3dd4ec69e36137904d5850a |
| institution | OA Journals |
| issn | 1933-6918 1933-6926 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cell Adhesion & Migration |
| spelling | doaj-art-87378046d3dd4ec69e36137904d5850a2025-08-20T02:31:13ZengTaylor & Francis GroupCell Adhesion & Migration1933-69181933-69262025-12-0119111110.1080/19336918.2024.2434209Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancerZhe Liu0Xiaofeng Pu1Department of Urology, Wuxi No. 2 People’s Hospital (Jiangnan University Medical Center), Wuxi, ChinaDepartment of Urology, Chongqing General Hospital, Chongqing University, Chongqing, ChinaThe research endeavors to expound the role of ORM1 in bladder cancer (BCa) and the implied response mechanism. RT-qPCR and Western blotting examined ORM1 and S100A12 expression. Functional experiments assessed the cellular phenotypes. HDOCK and Co-IP confirmed the interaction of ORM1 and S100A12. Western blotting tested apoptosis- and ERK signaling-associated proteins. ORM1 and S100A12 were abundant in the BCa cells. ORM1 or S100A12 loss impaired cell proliferation, migration, and invasion, and aggravated cell apoptosis. ORM1 interacted with S100A12. ORM1 knockdown down-regulated S100A12 expression and inactivated ERK signaling.S100A12 overexpression or ERK activator reversed the impacts of ORM1 interference on ERK signaling and BCa cells. ORM1 mightdrive BCa via binding to S100A12 and activating ERK signaling.https://www.tandfonline.com/doi/10.1080/19336918.2024.2434209Extracellular signal-regulated kinasemetastasisorosomucoid 1S100 calcium binding protein A12urinary bladder neoplasm |
| spellingShingle | Zhe Liu Xiaofeng Pu Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer Cell Adhesion & Migration Extracellular signal-regulated kinase metastasis orosomucoid 1 S100 calcium binding protein A12 urinary bladder neoplasm |
| title | Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer |
| title_full | Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer |
| title_fullStr | Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer |
| title_full_unstemmed | Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer |
| title_short | Orosomucoid 1 interacts with S100A12 and activates ERK signalling to expedite the advancement of bladder cancer |
| title_sort | orosomucoid 1 interacts with s100a12 and activates erk signalling to expedite the advancement of bladder cancer |
| topic | Extracellular signal-regulated kinase metastasis orosomucoid 1 S100 calcium binding protein A12 urinary bladder neoplasm |
| url | https://www.tandfonline.com/doi/10.1080/19336918.2024.2434209 |
| work_keys_str_mv | AT zheliu orosomucoid1interactswiths100a12andactivateserksignallingtoexpeditetheadvancementofbladdercancer AT xiaofengpu orosomucoid1interactswiths100a12andactivateserksignallingtoexpeditetheadvancementofbladdercancer |