Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis
ABSTRACT Background Testing for targeting programmed death ligand 1 (PD‐L1) is standard of care for patients with newly diagnosed non‐small cell lung cancer (NSCLC) but is only approved for use with core biopsy specimens. Endobronchial ultrasound guided miniforceps biopsy (EBUS‐MFB) is an approach t...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15502 |
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| author | Max T. Wayne Nathaniel G. Moulton Cody Weimholt Praveen Chenna Alexander C. Chen |
| author_facet | Max T. Wayne Nathaniel G. Moulton Cody Weimholt Praveen Chenna Alexander C. Chen |
| author_sort | Max T. Wayne |
| collection | DOAJ |
| description | ABSTRACT Background Testing for targeting programmed death ligand 1 (PD‐L1) is standard of care for patients with newly diagnosed non‐small cell lung cancer (NSCLC) but is only approved for use with core biopsy specimens. Endobronchial ultrasound guided miniforceps biopsy (EBUS‐MFB) is an approach to obtain core biopsy material but data assessing the ability of EBUS‐MFB to adequately test for PD‐L1 is lacking. We evaluate the feasibility of EBUS‐MFB to acquire adequate tissue for PD‐L1 testing and look to compare the quality of specimens between EBUS‐MFB and endobronchial ultrasound guided transbronchial needle aspiration (EBUS‐TBNA) using a standard method of tissue analysis. Methods Twenty patients with suspected non‐small cell lung cancer undergoing bronchoscopy were recruited for enrollment. For each patient with NSCLC diagnosed on rapid onsite pathology with EBUS‐TBNA, EBUS‐MFB was performed. PD‐L1 immunostaining was completed to assess for adequacy. A comparison of tissue collection was performed using the total surface area measured by digital imaging. Results Among 20 patients, 65% were male with a mean age of 66 years with a total procedure time of 50 min and an average of 14 biopsy passes per procedure. 15 (75%) patients were diagnosed with NSCLC, and PD‐L1 analysis was successfully performed in 12 of the 15 (80%). The mean total tissue area obtained by the MFB technique was 9.757 mm2 compared to 6.941 mm2 with TBNA (p = 0.427). Conclusion In this feasibility study, EBUS‐MFB was successful in performing PD‐L1 testing in 80% of patients with NSCLC. |
| format | Article |
| id | doaj-art-873455bf78b74888bbf55dfd8482f335 |
| institution | Kabale University |
| issn | 1759-7706 1759-7714 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj-art-873455bf78b74888bbf55dfd8482f3352025-01-30T22:40:34ZengWileyThoracic Cancer1759-77061759-77142025-01-01162n/an/a10.1111/1759-7714.15502Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue AnalysisMax T. Wayne0Nathaniel G. Moulton1Cody Weimholt2Praveen Chenna3Alexander C. Chen4Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine University of Michigan Ann Arbor Michigan USADivision of Pulmonary and Critical Care, Department of Internal Medicine Washington University in St. Louis St Louis Missouri USADivision of Anatomic and Molecular Pathology Washington University School of Medicine St. Louis Missouri USADivision of Pulmonary and Critical Care, Department of Internal Medicine Washington University in St. Louis St Louis Missouri USADivision of Pulmonary and Critical Care, Department of Internal Medicine Washington University in St. Louis St Louis Missouri USAABSTRACT Background Testing for targeting programmed death ligand 1 (PD‐L1) is standard of care for patients with newly diagnosed non‐small cell lung cancer (NSCLC) but is only approved for use with core biopsy specimens. Endobronchial ultrasound guided miniforceps biopsy (EBUS‐MFB) is an approach to obtain core biopsy material but data assessing the ability of EBUS‐MFB to adequately test for PD‐L1 is lacking. We evaluate the feasibility of EBUS‐MFB to acquire adequate tissue for PD‐L1 testing and look to compare the quality of specimens between EBUS‐MFB and endobronchial ultrasound guided transbronchial needle aspiration (EBUS‐TBNA) using a standard method of tissue analysis. Methods Twenty patients with suspected non‐small cell lung cancer undergoing bronchoscopy were recruited for enrollment. For each patient with NSCLC diagnosed on rapid onsite pathology with EBUS‐TBNA, EBUS‐MFB was performed. PD‐L1 immunostaining was completed to assess for adequacy. A comparison of tissue collection was performed using the total surface area measured by digital imaging. Results Among 20 patients, 65% were male with a mean age of 66 years with a total procedure time of 50 min and an average of 14 biopsy passes per procedure. 15 (75%) patients were diagnosed with NSCLC, and PD‐L1 analysis was successfully performed in 12 of the 15 (80%). The mean total tissue area obtained by the MFB technique was 9.757 mm2 compared to 6.941 mm2 with TBNA (p = 0.427). Conclusion In this feasibility study, EBUS‐MFB was successful in performing PD‐L1 testing in 80% of patients with NSCLC.https://doi.org/10.1111/1759-7714.15502biopsybronchoscopycytopathologynon‐small cell lung cancerPD‐L1 |
| spellingShingle | Max T. Wayne Nathaniel G. Moulton Cody Weimholt Praveen Chenna Alexander C. Chen Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis Thoracic Cancer biopsy bronchoscopy cytopathology non‐small cell lung cancer PD‐L1 |
| title | Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis |
| title_full | Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis |
| title_fullStr | Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis |
| title_full_unstemmed | Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis |
| title_short | Feasibility of Endobronchial Ultrasound Guided Miniforceps Biopsy for PD‐L1 Expression and Quantitative Tissue Analysis |
| title_sort | feasibility of endobronchial ultrasound guided miniforceps biopsy for pd l1 expression and quantitative tissue analysis |
| topic | biopsy bronchoscopy cytopathology non‐small cell lung cancer PD‐L1 |
| url | https://doi.org/10.1111/1759-7714.15502 |
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