Association of Enzyme Inhibition with Methods of Museum Skin Preparation
Enzyme inhibition is commonly encountered when using molecular biological techniques on museum-prepared animal skin samples, and this problem is exacerbated by a lack of information on how particular skins have been prepared for preservation. This report: (i) demonstrates that while some methods of...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1997-05-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/97225st07 |
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| Summary: | Enzyme inhibition is commonly encountered when using molecular biological techniques on museum-prepared animal skin samples, and this problem is exacerbated by a lack of information on how particular skins have been prepared for preservation. This report: (i) demonstrates that while some methods of museum preparation inhibit both proteinase K digestion and the PCR, others do not; (ii) describes a change in buffer conditions that reduces proteinase K enzyme inhibition during tissue digestion; and (iii) uses electron-dispersive X-ray microanalysis (EDXA) to show that the preparation methods for museum-preserved skin are often more complex than the treatment description provided with samples and also suggests that some of these descriptions are incorrect. |
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| ISSN: | 0736-6205 1940-9818 |