Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae)
Leishmania (L.) chagasi is the etiological agent of visceral leishmaniasis, an important endemic zoonosis in the American continent, as well as in many other countries in Asia, Africa, and Mediterranean Europe. The treatment is difficult due to the high toxicity of the available drugs, high costs, a...
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/516408 |
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| author | Eliete Cavalcanti da Silva Cynthia Dias Rayol Paloma Lys Medeiros Regina Célia Bressan Queiroz Figueiredo Márcia Regina Piuvezan José Maria Brabosa-Filho Alexsandro Fernandes Marinho Teresinha Gonçalves Silva Gardenia Carmen Gadelha Militão Ana Paula Pimentel Cassilhas Paulo Paes de Andrade |
| author_facet | Eliete Cavalcanti da Silva Cynthia Dias Rayol Paloma Lys Medeiros Regina Célia Bressan Queiroz Figueiredo Márcia Regina Piuvezan José Maria Brabosa-Filho Alexsandro Fernandes Marinho Teresinha Gonçalves Silva Gardenia Carmen Gadelha Militão Ana Paula Pimentel Cassilhas Paulo Paes de Andrade |
| author_sort | Eliete Cavalcanti da Silva |
| collection | DOAJ |
| description | Leishmania (L.) chagasi is the etiological agent of visceral leishmaniasis, an important endemic zoonosis in the American continent, as well as in many other countries in Asia, Africa, and Mediterranean Europe. The treatment is difficult due to the high toxicity of the available drugs, high costs, and emergence of resistance in the parasites. Therefore, there is an urgent need for new leishmanicidal agents. The bisbenzylisoquinoline alkaloids have been related to antibacterial, antiprotozoal, and antifungal activities. The aim of this study was to evaluate the growth inhibitory activity of warifteine (bisbenzylisoquinoline alkaloid) against L. chagasi promastigotes in axenic cultures and the occurrence of drug-induced ultrastructural changes in the parasite. This bisbenzylisoquinoline alkaloid was isolated from the leaves and roots of Cissampelos sympodialis Eichl. (Menispermaceae), a plant commonly used for the treatment of various diseases in Brazilian folk medicine. Using the purified warifteine, the 50% inhibitory concentration (IC50) was determined at 0.08 mg/mL after 72 h in culture, inducing significant changes in the parasite morphology, like aberrant multisepted forms and blebs in the plasma membrane. In conclusion, warifteine represents an attractive candidate for future pharmacological studies aiming new leishmanicidal drugs. |
| format | Article |
| id | doaj-art-8724a49ed7064fe0a9b15a7f2934c544 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8724a49ed7064fe0a9b15a7f2934c5442025-02-03T06:11:31ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/516408516408Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae)Eliete Cavalcanti da Silva0Cynthia Dias Rayol1Paloma Lys Medeiros2Regina Célia Bressan Queiroz Figueiredo3Márcia Regina Piuvezan4José Maria Brabosa-Filho5Alexsandro Fernandes Marinho6Teresinha Gonçalves Silva7Gardenia Carmen Gadelha Militão8Ana Paula Pimentel Cassilhas9Paulo Paes de Andrade10Departamento de Histologia e Embriologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Histologia e Embriologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Histologia e Embriologia, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Microbiologia, Centro de Pesquisas Aggeu Magalhães/FIOCRUZ, 50670-420 Recife, PE, BrazilLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Campus I, 58051-900 João Pessoa, PB, BrazilLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Campus I, 58051-900 João Pessoa, PB, BrazilLaboratório de Tecnologia Farmacêutica, Universidade Federal da Paraíba, Campus I, 58051-900 João Pessoa, PB, BrazilDepartamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Antibióticos, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego, 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilDepartamento de Ensino de Enfermagem, Universidade Salgado de Oliveira, 51170-000 Recife, PE, BrazilDepartamento de Genética, Centro de Ciências Biológicas, Universidade Federal de Pernambuco, Avenida Professor Moraes Rego 1265, Cidade Universitária, 50670-901 Recife, PE, BrazilLeishmania (L.) chagasi is the etiological agent of visceral leishmaniasis, an important endemic zoonosis in the American continent, as well as in many other countries in Asia, Africa, and Mediterranean Europe. The treatment is difficult due to the high toxicity of the available drugs, high costs, and emergence of resistance in the parasites. Therefore, there is an urgent need for new leishmanicidal agents. The bisbenzylisoquinoline alkaloids have been related to antibacterial, antiprotozoal, and antifungal activities. The aim of this study was to evaluate the growth inhibitory activity of warifteine (bisbenzylisoquinoline alkaloid) against L. chagasi promastigotes in axenic cultures and the occurrence of drug-induced ultrastructural changes in the parasite. This bisbenzylisoquinoline alkaloid was isolated from the leaves and roots of Cissampelos sympodialis Eichl. (Menispermaceae), a plant commonly used for the treatment of various diseases in Brazilian folk medicine. Using the purified warifteine, the 50% inhibitory concentration (IC50) was determined at 0.08 mg/mL after 72 h in culture, inducing significant changes in the parasite morphology, like aberrant multisepted forms and blebs in the plasma membrane. In conclusion, warifteine represents an attractive candidate for future pharmacological studies aiming new leishmanicidal drugs.http://dx.doi.org/10.1100/2012/516408 |
| spellingShingle | Eliete Cavalcanti da Silva Cynthia Dias Rayol Paloma Lys Medeiros Regina Célia Bressan Queiroz Figueiredo Márcia Regina Piuvezan José Maria Brabosa-Filho Alexsandro Fernandes Marinho Teresinha Gonçalves Silva Gardenia Carmen Gadelha Militão Ana Paula Pimentel Cassilhas Paulo Paes de Andrade Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) The Scientific World Journal |
| title | Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) |
| title_full | Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) |
| title_fullStr | Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) |
| title_full_unstemmed | Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) |
| title_short | Antileishmanial Activity of Warifteine: A Bisbenzylisoquinoline Alkaloid Isolated from Cissampelos sympodialis Eichl. (Menispermaceae) |
| title_sort | antileishmanial activity of warifteine a bisbenzylisoquinoline alkaloid isolated from cissampelos sympodialis eichl menispermaceae |
| url | http://dx.doi.org/10.1100/2012/516408 |
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