A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts
Abstract Premise Leaf extracts are valuable sources of bioactive compounds. However, co‐extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal often have limitations, including the use of hazardous solvents, low specif...
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| Format: | Article |
| Language: | English |
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Wiley
2025-07-01
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| Series: | Applications in Plant Sciences |
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| Online Access: | https://doi.org/10.1002/aps3.70018 |
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| author | Alice Fossati Valeria Cavalloro Daniela Rossi Simona Collina Emanuela Martino |
| author_facet | Alice Fossati Valeria Cavalloro Daniela Rossi Simona Collina Emanuela Martino |
| author_sort | Alice Fossati |
| collection | DOAJ |
| description | Abstract Premise Leaf extracts are valuable sources of bioactive compounds. However, co‐extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal often have limitations, including the use of hazardous solvents, low specificity, or high costs. Methods and Results A solid‐phase extraction protocol for chlorophyll removal from leaf extracts of Corylus avellana was developed using commercially available cartridges. The method requires standard equipment, can be completed within 10 minutes, and is scalable from analytical to preparative quantities. We validated this protocol across 20 taxa, demonstrating the removal of 85% of chlorophylls, successful scale up of quantities, cartridge reusability, and low solvent consumption. Conclusions Key innovations of the protocol include simplified elution steps and the possibility of multiple reuse cycles. The simplicity, sustainability, and scalability of this new protocol make it particularly valuable for high‐throughput applications and process development. |
| format | Article |
| id | doaj-art-871d6881946e46bb9eadaf389fc2a7c4 |
| institution | Kabale University |
| issn | 2168-0450 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj-art-871d6881946e46bb9eadaf389fc2a7c42025-08-20T03:57:09ZengWileyApplications in Plant Sciences2168-04502025-07-01134n/an/a10.1002/aps3.70018A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extractsAlice Fossati0Valeria Cavalloro1Daniela Rossi2Simona Collina3Emanuela Martino4Department of Earth and Environmental Sciences University of Pavia Via Ferrata 1 Pavia 27100 ItalyDepartment of Earth and Environmental Sciences University of Pavia Via Ferrata 1 Pavia 27100 ItalyDepartment of Drug Science University of Pavia Via Taramelli 12 Pavia 27100 ItalyDepartment of Drug Science University of Pavia Via Taramelli 12 Pavia 27100 ItalyDepartment of Earth and Environmental Sciences University of Pavia Via Ferrata 1 Pavia 27100 ItalyAbstract Premise Leaf extracts are valuable sources of bioactive compounds. However, co‐extracted chlorophylls interfere with analyses, including spectroscopic and biochemical assays. Existing methods for chlorophyll removal often have limitations, including the use of hazardous solvents, low specificity, or high costs. Methods and Results A solid‐phase extraction protocol for chlorophyll removal from leaf extracts of Corylus avellana was developed using commercially available cartridges. The method requires standard equipment, can be completed within 10 minutes, and is scalable from analytical to preparative quantities. We validated this protocol across 20 taxa, demonstrating the removal of 85% of chlorophylls, successful scale up of quantities, cartridge reusability, and low solvent consumption. Conclusions Key innovations of the protocol include simplified elution steps and the possibility of multiple reuse cycles. The simplicity, sustainability, and scalability of this new protocol make it particularly valuable for high‐throughput applications and process development.https://doi.org/10.1002/aps3.70018chlorophyllsinterferent removalnature‐aided drug discoverypolyphenolssolid‐phase extraction |
| spellingShingle | Alice Fossati Valeria Cavalloro Daniela Rossi Simona Collina Emanuela Martino A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts Applications in Plant Sciences chlorophylls interferent removal nature‐aided drug discovery polyphenols solid‐phase extraction |
| title | A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts |
| title_full | A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts |
| title_fullStr | A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts |
| title_full_unstemmed | A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts |
| title_short | A practical and easy‐to‐scale protocol for removing chlorophylls from leaf extracts |
| title_sort | practical and easy to scale protocol for removing chlorophylls from leaf extracts |
| topic | chlorophylls interferent removal nature‐aided drug discovery polyphenols solid‐phase extraction |
| url | https://doi.org/10.1002/aps3.70018 |
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