Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models
The hippocampus plays an important role in the genesis of mesial temporal lobe epilepsy, and the entorhinal cortex (EC) may affect the hippocampal network activity because of the heavy interconnection between them. However, the mechanism by which the EC affects the discharge patterns and the transmi...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2014/205912 |
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| author | Ye-Jun Shi Xin-Wei Gong Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu |
| author_facet | Ye-Jun Shi Xin-Wei Gong Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu |
| author_sort | Ye-Jun Shi |
| collection | DOAJ |
| description | The hippocampus plays an important role in the genesis of mesial temporal lobe epilepsy, and the entorhinal cortex (EC) may affect the hippocampal network activity because of the heavy interconnection between them. However, the mechanism by which the EC affects the discharge patterns and the transmission mode of epileptiform discharges within the hippocampus needs further study. Here, multielectrode recording techniques were used to study the spatiotemporal characteristics of epileptiform discharges in adult mouse hippocampal slices and combined EC-hippocampal slices and determine whether and how the EC affects the hippocampal neuron discharge patterns. The results showed that low-Mg2+ artificial cerebrospinal fluid induced interictal discharges in hippocampal slices, whereas, in combined EC-hippocampal slices the discharge pattern was alternated between interictal and ictal discharges, and ictal discharges initiated in the EC and propagated to the hippocampus. The pharmacological effect of the antiepileptic drug valproate (VPA) was tested. VPA reversibly suppressed the frequency of interictal discharges but did not change the initiation site and propagation speed, and it completely blocked ictal discharges. Our results suggested that EC was necessary for the hippocampal ictal discharges, and ictal discharges were more sensitive than interictal discharges in response to VPA. |
| format | Article |
| id | doaj-art-87199f2de8c049bdaa056f8b00c4d01a |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-87199f2de8c049bdaa056f8b00c4d01a2025-02-03T07:25:58ZengWileyNeural Plasticity2090-59041687-54432014-01-01201410.1155/2014/205912205912Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice ModelsYe-Jun Shi0Xin-Wei Gong1Hai-Qing Gong2Pei-Ji Liang3Pu-Ming Zhang4Qin-Chi Lu5Department of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dong-Fang Road, Shanghai 200127, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, ChinaSchool of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dong-Chuan Road, Shanghai 200240, ChinaDepartment of Neurology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, 1630 Dong-Fang Road, Shanghai 200127, ChinaThe hippocampus plays an important role in the genesis of mesial temporal lobe epilepsy, and the entorhinal cortex (EC) may affect the hippocampal network activity because of the heavy interconnection between them. However, the mechanism by which the EC affects the discharge patterns and the transmission mode of epileptiform discharges within the hippocampus needs further study. Here, multielectrode recording techniques were used to study the spatiotemporal characteristics of epileptiform discharges in adult mouse hippocampal slices and combined EC-hippocampal slices and determine whether and how the EC affects the hippocampal neuron discharge patterns. The results showed that low-Mg2+ artificial cerebrospinal fluid induced interictal discharges in hippocampal slices, whereas, in combined EC-hippocampal slices the discharge pattern was alternated between interictal and ictal discharges, and ictal discharges initiated in the EC and propagated to the hippocampus. The pharmacological effect of the antiepileptic drug valproate (VPA) was tested. VPA reversibly suppressed the frequency of interictal discharges but did not change the initiation site and propagation speed, and it completely blocked ictal discharges. Our results suggested that EC was necessary for the hippocampal ictal discharges, and ictal discharges were more sensitive than interictal discharges in response to VPA.http://dx.doi.org/10.1155/2014/205912 |
| spellingShingle | Ye-Jun Shi Xin-Wei Gong Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models Neural Plasticity |
| title | Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models |
| title_full | Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models |
| title_fullStr | Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models |
| title_full_unstemmed | Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models |
| title_short | Effect of the Entorhinal Cortex on Ictal Discharges in Low-Mg2+-Induced Epileptic Hippocampal Slice Models |
| title_sort | effect of the entorhinal cortex on ictal discharges in low mg2 induced epileptic hippocampal slice models |
| url | http://dx.doi.org/10.1155/2014/205912 |
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