Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration
Aim. This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems. Methods. At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a s...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2021/8840570 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850215621122326528 |
|---|---|
| author | Eliane Avany Malveira Araújo Geisy Rebouças Lima Luciana Aleixo dos Santos de Melo Leilane Bentes de Sousa Marne Carvalho de Vasconcellos Nikeila Chacon de Oliveira Conde Carina Toda Simone Assayag Hanan Ary de Oliveira Alves Filho Maria Fulgência Costa Lima Bandeira |
| author_facet | Eliane Avany Malveira Araújo Geisy Rebouças Lima Luciana Aleixo dos Santos de Melo Leilane Bentes de Sousa Marne Carvalho de Vasconcellos Nikeila Chacon de Oliveira Conde Carina Toda Simone Assayag Hanan Ary de Oliveira Alves Filho Maria Fulgência Costa Lima Bandeira |
| author_sort | Eliane Avany Malveira Araújo |
| collection | DOAJ |
| description | Aim. This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems. Methods. At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a smear layer was standardized and subdivided into four groups. Gelatin conjugated with fluorescein was used and taken to the fluorescence microscope for evaluation. In cytotoxicity, the Trypan Blue method was used at four different time points. The tested groups were (G1) control with distilled water; (G2) 2% chlorhexidine (CLX); (G3) emulsion based on copaiba oil (EC) 10% + X; (G4) 10% EC + Y; and (G5) EC 10% alkaline. The zymographic assay used the same groups described, but in 30 seconds and 10 and 20 minutes. HT1080 cells were incubated and submitted to electrophoresis. The gel was analyzed using ImageJ software. Mann–Whitney and Kruskal–Wallis tests were used in the statistical analysis (p<0.05). Results. ECs showed higher cell viability in the cytotoxicity test and showed a significant difference in 10 and 20 minutes. In the zymographic assay, alkaline EC reduced 67% of MMP-2 activity and 44% of MMP-9 compared to 2% chlorhexidine. At in situ zymography in qualitative evaluation, all groups tested showed inhibition of activity in metalloproteinases. Conclusion. EC showed activity in the inhibition of metalloproteinases in vitro and in situ, especially the alkaline one. The survey shows the possibility of using ECs, a product from Amazonian biodiversity, as a biomodifier in dentistry. |
| format | Article |
| id | doaj-art-8704c8f320fa408081483cb15a6c5dd3 |
| institution | OA Journals |
| issn | 2633-4690 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-8704c8f320fa408081483cb15a6c5dd32025-08-20T02:08:35ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902021-01-01202110.1155/2021/88405708840570Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive RestorationEliane Avany Malveira Araújo0Geisy Rebouças Lima1Luciana Aleixo dos Santos de Melo2Leilane Bentes de Sousa3Marne Carvalho de Vasconcellos4Nikeila Chacon de Oliveira Conde5Carina Toda6Simone Assayag Hanan7Ary de Oliveira Alves Filho8Maria Fulgência Costa Lima Bandeira9School of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Pharmaceutical Sciences, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Pharmaceutical Sciences, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Pharmaceutical Sciences, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Pharmaceutical Sciences, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilSchool of Dentistry, Amazonas Federal University,UFAM, Manaus, AM, BrazilAim. This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems. Methods. At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a smear layer was standardized and subdivided into four groups. Gelatin conjugated with fluorescein was used and taken to the fluorescence microscope for evaluation. In cytotoxicity, the Trypan Blue method was used at four different time points. The tested groups were (G1) control with distilled water; (G2) 2% chlorhexidine (CLX); (G3) emulsion based on copaiba oil (EC) 10% + X; (G4) 10% EC + Y; and (G5) EC 10% alkaline. The zymographic assay used the same groups described, but in 30 seconds and 10 and 20 minutes. HT1080 cells were incubated and submitted to electrophoresis. The gel was analyzed using ImageJ software. Mann–Whitney and Kruskal–Wallis tests were used in the statistical analysis (p<0.05). Results. ECs showed higher cell viability in the cytotoxicity test and showed a significant difference in 10 and 20 minutes. In the zymographic assay, alkaline EC reduced 67% of MMP-2 activity and 44% of MMP-9 compared to 2% chlorhexidine. At in situ zymography in qualitative evaluation, all groups tested showed inhibition of activity in metalloproteinases. Conclusion. EC showed activity in the inhibition of metalloproteinases in vitro and in situ, especially the alkaline one. The survey shows the possibility of using ECs, a product from Amazonian biodiversity, as a biomodifier in dentistry.http://dx.doi.org/10.1155/2021/8840570 |
| spellingShingle | Eliane Avany Malveira Araújo Geisy Rebouças Lima Luciana Aleixo dos Santos de Melo Leilane Bentes de Sousa Marne Carvalho de Vasconcellos Nikeila Chacon de Oliveira Conde Carina Toda Simone Assayag Hanan Ary de Oliveira Alves Filho Maria Fulgência Costa Lima Bandeira Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration Advances in Pharmacological and Pharmaceutical Sciences |
| title | Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration |
| title_full | Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration |
| title_fullStr | Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration |
| title_full_unstemmed | Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration |
| title_short | Effect of a Copaiba Oil-Based Dental Biomodifier on the Inhibition of Metalloproteinase in Adhesive Restoration |
| title_sort | effect of a copaiba oil based dental biomodifier on the inhibition of metalloproteinase in adhesive restoration |
| url | http://dx.doi.org/10.1155/2021/8840570 |
| work_keys_str_mv | AT elianeavanymalveiraaraujo effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT geisyreboucaslima effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT lucianaaleixodossantosdemelo effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT leilanebentesdesousa effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT marnecarvalhodevasconcellos effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT nikeilachacondeoliveiraconde effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT carinatoda effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT simoneassayaghanan effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT arydeoliveiraalvesfilho effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration AT mariafulgenciacostalimabandeira effectofacopaibaoilbaseddentalbiomodifierontheinhibitionofmetalloproteinaseinadhesiverestoration |