Scan-rescan reliability assessment of brain volumetric analysis across scanners and software solutions

Abstract Automated brain volumetry shows promise in improving the screening and monitoring of neurodegenerative diseases. However, the reliability of measurements across different scanners and software remains uncertain. This study assessed the reliability of gray matter, white matter, and total bra...

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Bibliographic Details
Main Authors: Eva Bürkle, Ahmad Nazzal, Alexander Debolski, Ulrike Ernemann, Tobias Lindig, Benjamin Bender
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-025-15283-3
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Summary:Abstract Automated brain volumetry shows promise in improving the screening and monitoring of neurodegenerative diseases. However, the reliability of measurements across different scanners and software remains uncertain. This study assessed the reliability of gray matter, white matter, and total brain volume measurements from seven volumetry tools, using six scanners across two scanning sessions, performed within 2 h the same day, in twelve subjects. Generalised estimating equations models showed significant effects of both software and scanner on all measurements with stronger effect of software (p < 0.001). Percentage of coefficient of variation (CV) was calculated to measure scan-rescan reliability. Median CV across scanners of AssemblyNet and AIRAscore was less than 0.2% for grey and white matter, and 0.09% for total brain volume; while FreeSurfer, FastSurfer, syngo.via, SPM12, and Vol2Brain had a CV greater than 0.2%. In Bland-Altman analysis there was no systematic difference, but limits of agreement differed greatly between methods. Based on these findings, we recommend using the same scanner and software combination across sessions to ensure that observed changes in brain volume are reliable and clinically valuable.
ISSN:2045-2322