Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking

Coconut endosperm residue is a rich dietary fiber resource; however, its hydration properties are poor. To enhance the functionality and applications of coconut endosperm residue dietary fiber (CERDF) in the food industry, ultrasound, cellulase, and hemicellulase hydrolysis combined with carboxymeth...

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Main Authors: Anyu Zhang, Jun Ma, Peiyao Long, Yajun Zheng, Yichan Zhang
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Food Science
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Online Access:http://www.sciencedirect.com/science/article/pii/S2665927124002685
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author Anyu Zhang
Jun Ma
Peiyao Long
Yajun Zheng
Yichan Zhang
author_facet Anyu Zhang
Jun Ma
Peiyao Long
Yajun Zheng
Yichan Zhang
author_sort Anyu Zhang
collection DOAJ
description Coconut endosperm residue is a rich dietary fiber resource; however, its hydration properties are poor. To enhance the functionality and applications of coconut endosperm residue dietary fiber (CERDF) in the food industry, ultrasound, cellulase, and hemicellulase hydrolysis combined with carboxymethylation or phosphate crosslinking have been used. The impact of the modified CERDFs on egg white protein gel (EWPG) was also studied. Compared to unmodified CERDF, CERDF modified by ultrasound and dual enzymatic hydrolysis combined with carboxymethylation (CERDF-UDEC) or phosphate-crosslinking (CERDF-UDEPC) exhibited a larger surface area and improved water retention and expansion abilities (p < 0.05). Addition of CERDF, CERDF-UDEC, and CERDF-UDEPC increased the random coil content of EWPG and rendered its microstructure more granular. CERDF-UDEC and CERDF-UDEPC improved EWPG properties more effectively than unmodified CERDF. These enhancements included increased water retention, pH, hardness (from 109.87 to 222.38 g), chewiness (from 78.07 to 172.13 g), and gumminess (from 85.12 to 181.82), and a reduction in its freeze-thaw dehydration rate (from 33.66% to 16.26%) and transparency (p < 0.05). Adding CERDF and CERDF-UDEC (3–5 g/100 g) enhanced the gastric stability and intestinal digestibility of EWPG. Thus, CERDF modified through ultrasound and dual enzymolysis combined with carboxymethylation or crosslinking improved the gel properties of EWPG. However, further research is needed to clarify the mechanisms behind these modifications and evaluate their economic feasibility.
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spelling doaj-art-86f3fcbed2f94bf2b2fd3a4c197d1bed2025-08-20T02:38:06ZengElsevierCurrent Research in Food Science2665-92712024-01-01910094110.1016/j.crfs.2024.100941Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinkingAnyu Zhang0Jun Ma1Peiyao Long2Yajun Zheng3Yichan Zhang4Food Science College of Shanxi Normal University, Taiyuan, 030092, ChinaShanxi Province Cancer Hospital, Taiyuan, 030013, China; Shanxi Hospital Affiliated to Cancer Hospital, Chinese Academy of Medical Sciences, Taiyuan, 030013, China; Cancer Hospital Affiliated to Shanxi Medical University, Taiyuan, 030013, ChinaFood Science College of Shanxi Normal University, Taiyuan, 030092, ChinaFood Science College of Shanxi Normal University, Taiyuan, 030092, China; Corresponding author. Shanxi Normal University, Taiyuan, 030092. ChinaFood Science College of Shanxi Normal University, Taiyuan, 030092, ChinaCoconut endosperm residue is a rich dietary fiber resource; however, its hydration properties are poor. To enhance the functionality and applications of coconut endosperm residue dietary fiber (CERDF) in the food industry, ultrasound, cellulase, and hemicellulase hydrolysis combined with carboxymethylation or phosphate crosslinking have been used. The impact of the modified CERDFs on egg white protein gel (EWPG) was also studied. Compared to unmodified CERDF, CERDF modified by ultrasound and dual enzymatic hydrolysis combined with carboxymethylation (CERDF-UDEC) or phosphate-crosslinking (CERDF-UDEPC) exhibited a larger surface area and improved water retention and expansion abilities (p < 0.05). Addition of CERDF, CERDF-UDEC, and CERDF-UDEPC increased the random coil content of EWPG and rendered its microstructure more granular. CERDF-UDEC and CERDF-UDEPC improved EWPG properties more effectively than unmodified CERDF. These enhancements included increased water retention, pH, hardness (from 109.87 to 222.38 g), chewiness (from 78.07 to 172.13 g), and gumminess (from 85.12 to 181.82), and a reduction in its freeze-thaw dehydration rate (from 33.66% to 16.26%) and transparency (p < 0.05). Adding CERDF and CERDF-UDEC (3–5 g/100 g) enhanced the gastric stability and intestinal digestibility of EWPG. Thus, CERDF modified through ultrasound and dual enzymolysis combined with carboxymethylation or crosslinking improved the gel properties of EWPG. However, further research is needed to clarify the mechanisms behind these modifications and evaluate their economic feasibility.http://www.sciencedirect.com/science/article/pii/S2665927124002685Coconut endosperm fiberUltrasonicDual enzymatic hydrolysisCrosslinkingCarboxymethylationEgg white protein gel
spellingShingle Anyu Zhang
Jun Ma
Peiyao Long
Yajun Zheng
Yichan Zhang
Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
Current Research in Food Science
Coconut endosperm fiber
Ultrasonic
Dual enzymatic hydrolysis
Crosslinking
Carboxymethylation
Egg white protein gel
title Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
title_full Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
title_fullStr Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
title_full_unstemmed Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
title_short Improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
title_sort improving gel properties of egg white protein using coconut endosperm dietary fibers modified by ultrasound and dual enzymolysis combined with carboxymethylation or phosphate crosslinking
topic Coconut endosperm fiber
Ultrasonic
Dual enzymatic hydrolysis
Crosslinking
Carboxymethylation
Egg white protein gel
url http://www.sciencedirect.com/science/article/pii/S2665927124002685
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