Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model

BackgroundZingiber officinale Roscoe has been shown to possess analgesic properties. Zingerone (ZO), a bioactive compound derived from Zingiber officinale Roscoe, exhibits a range of pharmacological effects, including anti-inflammatory, anti-cancer, antioxidant, antibacterial, and anti-apoptotic act...

Full description

Saved in:
Bibliographic Details
Main Authors: Yuanyuan Wang, Lang Dong, Shu Han, Yuehan You, Mingrui Zhang, Bingjing Sun, Hong Ni, Rongjing Ge, Jianhong Liu, Jiandong Yu
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-03-01
Series:Frontiers in Pharmacology
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fphar.2025.1543594/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850069500130492416
author Yuanyuan Wang
Yuanyuan Wang
Yuanyuan Wang
Lang Dong
Lang Dong
Shu Han
Yuehan You
Mingrui Zhang
Bingjing Sun
Bingjing Sun
Hong Ni
Hong Ni
Rongjing Ge
Rongjing Ge
Jianhong Liu
Jianhong Liu
Jiandong Yu
Jiandong Yu
Jiandong Yu
author_facet Yuanyuan Wang
Yuanyuan Wang
Yuanyuan Wang
Lang Dong
Lang Dong
Shu Han
Yuehan You
Mingrui Zhang
Bingjing Sun
Bingjing Sun
Hong Ni
Hong Ni
Rongjing Ge
Rongjing Ge
Jianhong Liu
Jianhong Liu
Jiandong Yu
Jiandong Yu
Jiandong Yu
author_sort Yuanyuan Wang
collection DOAJ
description BackgroundZingiber officinale Roscoe has been shown to possess analgesic properties. Zingerone (ZO), a bioactive compound derived from Zingiber officinale Roscoe, exhibits a range of pharmacological effects, including anti-inflammatory, anti-cancer, antioxidant, antibacterial, and anti-apoptotic activities. However, the analgesic properties of zingerone remain unclear.MethodsComplete Freund’s adjuvant (CFA) was administered to the left hind paw of C57BL/6 mice to induce a model of inflammatory pain. The analgesic effects of zingerone were assessed using the Von Frey and Hargreaves tests. In vivo fiber photometry and whole-cell patch clamp techniques were employed to investigate the potential mechanisms.ResultsBoth acute and long-term treatment with zingerone resulted in a significant increase in mechanical and thermal pain thresholds in mice experiencing CFA-induced inflammatory pain. Mechanical stimulation led to a pronounced increase in calcium levels within the anterior cingulate cortex (ACC) neurons of the inflammatory pain model, which was alleviated by zingerone administration. Furthermore, zingerone was found to modify synaptic transmission to ACC neurons and decrease their intrinsic excitability by prolonging the refractory period of these neurons.ConclusionZingerone demonstrates potential for alleviating CFA-induced inflammatory pain by reducing the intrinsic excitability of ACC neurons in a mouse model.
format Article
id doaj-art-86f12d59e2c24d0ba9347f7fc783bdf1
institution DOAJ
issn 1663-9812
language English
publishDate 2025-03-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Pharmacology
spelling doaj-art-86f12d59e2c24d0ba9347f7fc783bdf12025-08-20T02:47:46ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-03-011610.3389/fphar.2025.15435941543594Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice modelYuanyuan Wang0Yuanyuan Wang1Yuanyuan Wang2Lang Dong3Lang Dong4Shu Han5Yuehan You6Mingrui Zhang7Bingjing Sun8Bingjing Sun9Hong Ni10Hong Ni11Rongjing Ge12Rongjing Ge13Jianhong Liu14Jianhong Liu15Jiandong Yu16Jiandong Yu17Jiandong Yu18Department of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaSchool of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaSchool of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaSchool of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaSchool of Basic Medicine, Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Biochemistry and Molecular biology, School of Laboratory Medicine, Bengbu Medical University, Bengbu, Anhui, ChinaDepartment of Neurosurgery, the First Affiliated Hospital of Bengbu Medical University, Bengbu, Anhui, ChinaLaboratory of Brain and Psychiatric Disease, Bengbu Medical University, Bengbu, Anhui, ChinaAnhui Engineering Research Center for Neural Regeneration Technology and Medical New Materials, Bengbu Medical University, Bengbu, Anhui, ChinaBackgroundZingiber officinale Roscoe has been shown to possess analgesic properties. Zingerone (ZO), a bioactive compound derived from Zingiber officinale Roscoe, exhibits a range of pharmacological effects, including anti-inflammatory, anti-cancer, antioxidant, antibacterial, and anti-apoptotic activities. However, the analgesic properties of zingerone remain unclear.MethodsComplete Freund’s adjuvant (CFA) was administered to the left hind paw of C57BL/6 mice to induce a model of inflammatory pain. The analgesic effects of zingerone were assessed using the Von Frey and Hargreaves tests. In vivo fiber photometry and whole-cell patch clamp techniques were employed to investigate the potential mechanisms.ResultsBoth acute and long-term treatment with zingerone resulted in a significant increase in mechanical and thermal pain thresholds in mice experiencing CFA-induced inflammatory pain. Mechanical stimulation led to a pronounced increase in calcium levels within the anterior cingulate cortex (ACC) neurons of the inflammatory pain model, which was alleviated by zingerone administration. Furthermore, zingerone was found to modify synaptic transmission to ACC neurons and decrease their intrinsic excitability by prolonging the refractory period of these neurons.ConclusionZingerone demonstrates potential for alleviating CFA-induced inflammatory pain by reducing the intrinsic excitability of ACC neurons in a mouse model.https://www.frontiersin.org/articles/10.3389/fphar.2025.1543594/fullZingiber officinale roscoeanalgesicintrinsic excitabilitycomplete Freund’s adjuvant (CFA)refractory periodelectrophysiological
spellingShingle Yuanyuan Wang
Yuanyuan Wang
Yuanyuan Wang
Lang Dong
Lang Dong
Shu Han
Yuehan You
Mingrui Zhang
Bingjing Sun
Bingjing Sun
Hong Ni
Hong Ni
Rongjing Ge
Rongjing Ge
Jianhong Liu
Jianhong Liu
Jiandong Yu
Jiandong Yu
Jiandong Yu
Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
Frontiers in Pharmacology
Zingiber officinale roscoe
analgesic
intrinsic excitability
complete Freund’s adjuvant (CFA)
refractory period
electrophysiological
title Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
title_full Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
title_fullStr Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
title_full_unstemmed Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
title_short Zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
title_sort zingerone alleviates inflammatory pain by reducing the intrinsic excitability of anterior cingulate cortex neurons in a mice model
topic Zingiber officinale roscoe
analgesic
intrinsic excitability
complete Freund’s adjuvant (CFA)
refractory period
electrophysiological
url https://www.frontiersin.org/articles/10.3389/fphar.2025.1543594/full
work_keys_str_mv AT yuanyuanwang zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT yuanyuanwang zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT yuanyuanwang zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT langdong zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT langdong zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT shuhan zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT yuehanyou zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT mingruizhang zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT bingjingsun zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT bingjingsun zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT hongni zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT hongni zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT rongjingge zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT rongjingge zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT jianhongliu zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT jianhongliu zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT jiandongyu zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT jiandongyu zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel
AT jiandongyu zingeronealleviatesinflammatorypainbyreducingtheintrinsicexcitabilityofanteriorcingulatecortexneuronsinamicemodel