Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs
African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia and the Caribbean. The causative agent, ASF virus (ASFV), is a large, double-stranded, and structurally complex virus. The ASFV genome encodes for more than 160 pro...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/17/5/603 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849719618539618304 |
|---|---|
| author | Elizabeth Ramirez-Medina Paul A. Azzinaro Alyssa Valladares Ediane Silva Leeanna Burton Leandro Sastre Vivian O’Donnell James J. Zhu Douglas P. Gladue Manuel V. Borca |
| author_facet | Elizabeth Ramirez-Medina Paul A. Azzinaro Alyssa Valladares Ediane Silva Leeanna Burton Leandro Sastre Vivian O’Donnell James J. Zhu Douglas P. Gladue Manuel V. Borca |
| author_sort | Elizabeth Ramirez-Medina |
| collection | DOAJ |
| description | African swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia and the Caribbean. The causative agent, ASF virus (ASFV), is a large, double-stranded, and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of them are still in the process of being characterized. Recently, ASFV gene <i>I196L</i> has been reported as being critically involved in disease production in domestic pigs. We report here that a recombinant virus derived from the Georgia 2010 isolate (ASFV-G) lacking the <i>I196L</i> gene, ASFV-G-∆I196L, had the same ability to replicate in primary cultures of swine macrophage and, when experimentally inoculated in pigs, produced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of the <i>I196L</i> gene does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate. |
| format | Article |
| id | doaj-art-86eb3c95c71f47c8a5c37e6cd5d17959 |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-86eb3c95c71f47c8a5c37e6cd5d179592025-08-20T03:12:07ZengMDPI AGViruses1999-49152025-04-0117560310.3390/v17050603Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic PigsElizabeth Ramirez-Medina0Paul A. Azzinaro1Alyssa Valladares2Ediane Silva3Leeanna Burton4Leandro Sastre5Vivian O’Donnell6James J. Zhu7Douglas P. Gladue8Manuel V. Borca9Foreign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAU.S. Department of Agriculture, APHIS, Plum Island Animal Disease Center (PIADC), P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAForeign Animal Disease Research Unit, Plum Island Animal Disease Center (PIADC), Agricultural Research Service, U.S. Department of Agriculture, P.O. Box 848, Greenport, NY 11944, USAAfrican swine fever (ASF) is a lethal disease of domestic pigs that is currently challenging swine production in large areas of Eurasia and the Caribbean. The causative agent, ASF virus (ASFV), is a large, double-stranded, and structurally complex virus. The ASFV genome encodes for more than 160 proteins; however, the functions of most of them are still in the process of being characterized. Recently, ASFV gene <i>I196L</i> has been reported as being critically involved in disease production in domestic pigs. We report here that a recombinant virus derived from the Georgia 2010 isolate (ASFV-G) lacking the <i>I196L</i> gene, ASFV-G-∆I196L, had the same ability to replicate in primary cultures of swine macrophage and, when experimentally inoculated in pigs, produced a fatal form of the disease similar to that caused by the parental virulent ASFV-G. Therefore, deletion of the <i>I196L</i> gene does not significantly affect virus replication and virulence in domestic pigs of the ASFV Georgia 2010 isolate.https://www.mdpi.com/1999-4915/17/5/603ASFVASFAfrican swine fever virusrecombinant virusASFV virulenceASFV <i>I196L</i> gene |
| spellingShingle | Elizabeth Ramirez-Medina Paul A. Azzinaro Alyssa Valladares Ediane Silva Leeanna Burton Leandro Sastre Vivian O’Donnell James J. Zhu Douglas P. Gladue Manuel V. Borca Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs Viruses ASFV ASF African swine fever virus recombinant virus ASFV virulence ASFV <i>I196L</i> gene |
| title | Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs |
| title_full | Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs |
| title_fullStr | Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs |
| title_full_unstemmed | Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs |
| title_short | Deletion of the African Swine Fever Virus Gen <i>I196L</i> in the Georgia2010 Isolate Genome Does Not Affect Virus Replication or Virulence in Domestic Pigs |
| title_sort | deletion of the african swine fever virus gen i i196l i in the georgia2010 isolate genome does not affect virus replication or virulence in domestic pigs |
| topic | ASFV ASF African swine fever virus recombinant virus ASFV virulence ASFV <i>I196L</i> gene |
| url | https://www.mdpi.com/1999-4915/17/5/603 |
| work_keys_str_mv | AT elizabethramirezmedina deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT paulaazzinaro deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT alyssavalladares deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT edianesilva deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT leeannaburton deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT leandrosastre deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT vivianodonnell deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT jamesjzhu deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT douglaspgladue deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs AT manuelvborca deletionoftheafricanswinefevervirusgenii196liinthegeorgia2010isolategenomedoesnotaffectvirusreplicationorvirulenceindomesticpigs |