Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion
Background: Neutrophils are vital constituents of the immune response in the vaginal environment, playing a pivotal role in the defense against trichomoniasis. Earlier studies have shown that Trichomonas vaginalis (T. vaginalis) can release leukotriene B4 (LTB4), a molecule that attracts and activat...
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Elsevier
2025-02-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S168411822400210X |
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| author | Wei-Hung Cheng Ruei-Min Chen Seow-Chin Ong Yuan-Ming Yeh Po-Jung Huang Chi-Ching Lee |
| author_facet | Wei-Hung Cheng Ruei-Min Chen Seow-Chin Ong Yuan-Ming Yeh Po-Jung Huang Chi-Ching Lee |
| author_sort | Wei-Hung Cheng |
| collection | DOAJ |
| description | Background: Neutrophils are vital constituents of the immune response in the vaginal environment, playing a pivotal role in the defense against trichomoniasis. Earlier studies have shown that Trichomonas vaginalis (T. vaginalis) can release leukotriene B4 (LTB4), a molecule that attracts and activates neutrophils. Additionally, secretory products from this parasite can induce the production of interleukin-8 (IL-8) in mast cells and neutrophils, which further recruits neutrophils to the infection site. The precise reasons behind T. vaginalis actively promoting interaction between parasites and neutrophils rather than inhibiting the inflammatory response remain unclear. Results: In this study, we collected conditioned medium to elucidate the intricate dynamics between T. vaginalis and human neutrophils. We conducted a comprehensive profiling of soluble excretory/secretory proteins (ESPs), identifying 192 protein spots, of which 94 were successfully characterized through mass spectrometry analysis. Notably, the majority of induced ESPs from co-cultivation exhibited consistency with the trichomonad and neutrophil standalone groups, except for lactoferrin, which was observed exclusively following the interaction between neutrophils and T. vaginalis. The secretion of lactoferrin was determined to be a contact-dependent process. It was interesting to identify the ability of the iron-loaded lactoferrin to extend the survival time of T. vaginalis under iron-deficient conditions. Conclusions: This study represents the first to identify the origin of lactoferrin during T. vaginalis infection, shedding light on the potential reason for T. vaginalis's ability to attract neutrophils to the infection site: the acquisition of the iron source, lactoferrin. |
| format | Article |
| id | doaj-art-86ea68bf680847e183f2687736146649 |
| institution | Kabale University |
| issn | 1684-1182 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj-art-86ea68bf680847e183f26877361466492025-02-06T05:11:20ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822025-02-01581138147Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretionWei-Hung Cheng0Ruei-Min Chen1Seow-Chin Ong2Yuan-Ming Yeh3Po-Jung Huang4Chi-Ching Lee5Department of Parasitology, College of Medicine, National Cheng Kung University, Tainan, Taiwan; Corresponding author. Department of Parasitology, College of Medicine, National Cheng Kung University No. 1 University Road, Tainan City, 701, Taiwan.Department of Parasitology, College of Medicine, Chang Gung University, Guishan Dist., Taoyuan City, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Guishan Dist., Taoyuan City, TaiwanGenomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan; Graduate Institute of Health Industry Technology, Chang Gung University of Science and Technology, Taoyuan, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, TaiwanGenomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan; Department of Biomedical Sciences, College of Medicine, Chang Gung University, Guishan Dist., Taoyuan City, TaiwanGenomic Medicine Core Laboratory, Chang Gung Memorial Hospital, Linkou, Taiwan; Department of Computer Science and Information Engineering, College of Engineering, Chang Gung University, Guishan Dist., Taoyuan City, TaiwanBackground: Neutrophils are vital constituents of the immune response in the vaginal environment, playing a pivotal role in the defense against trichomoniasis. Earlier studies have shown that Trichomonas vaginalis (T. vaginalis) can release leukotriene B4 (LTB4), a molecule that attracts and activates neutrophils. Additionally, secretory products from this parasite can induce the production of interleukin-8 (IL-8) in mast cells and neutrophils, which further recruits neutrophils to the infection site. The precise reasons behind T. vaginalis actively promoting interaction between parasites and neutrophils rather than inhibiting the inflammatory response remain unclear. Results: In this study, we collected conditioned medium to elucidate the intricate dynamics between T. vaginalis and human neutrophils. We conducted a comprehensive profiling of soluble excretory/secretory proteins (ESPs), identifying 192 protein spots, of which 94 were successfully characterized through mass spectrometry analysis. Notably, the majority of induced ESPs from co-cultivation exhibited consistency with the trichomonad and neutrophil standalone groups, except for lactoferrin, which was observed exclusively following the interaction between neutrophils and T. vaginalis. The secretion of lactoferrin was determined to be a contact-dependent process. It was interesting to identify the ability of the iron-loaded lactoferrin to extend the survival time of T. vaginalis under iron-deficient conditions. Conclusions: This study represents the first to identify the origin of lactoferrin during T. vaginalis infection, shedding light on the potential reason for T. vaginalis's ability to attract neutrophils to the infection site: the acquisition of the iron source, lactoferrin.http://www.sciencedirect.com/science/article/pii/S168411822400210XTrichomonas vaginalisNeutrophilsExcretory-secretory proteinsCell-to-cell interactionProteomics analysisLactoferrin |
| spellingShingle | Wei-Hung Cheng Ruei-Min Chen Seow-Chin Ong Yuan-Ming Yeh Po-Jung Huang Chi-Ching Lee Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion Journal of Microbiology, Immunology and Infection Trichomonas vaginalis Neutrophils Excretory-secretory proteins Cell-to-cell interaction Proteomics analysis Lactoferrin |
| title | Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion |
| title_full | Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion |
| title_fullStr | Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion |
| title_full_unstemmed | Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion |
| title_short | Interaction of human neutrophils with Trichomonas vaginalis protozoan highlights lactoferrin secretion |
| title_sort | interaction of human neutrophils with trichomonas vaginalis protozoan highlights lactoferrin secretion |
| topic | Trichomonas vaginalis Neutrophils Excretory-secretory proteins Cell-to-cell interaction Proteomics analysis Lactoferrin |
| url | http://www.sciencedirect.com/science/article/pii/S168411822400210X |
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