The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis
Streptococcus suis is an important zoonotic pathogen that threatens human and pig health. During infection, the host can impose oxidative stress to resist pathogen invasion. Resistance to oxidative toxicity is an important factor for pathogens. Glutathione synthesis contributes to reactive oxygen sp...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Virulence |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2025.2474866 |
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| author | Wei Peng Qinggen Jiang Yuting Wu Li He Bei Li Weicheng Bei Xia Yang |
| author_facet | Wei Peng Qinggen Jiang Yuting Wu Li He Bei Li Weicheng Bei Xia Yang |
| author_sort | Wei Peng |
| collection | DOAJ |
| description | Streptococcus suis is an important zoonotic pathogen that threatens human and pig health. During infection, the host can impose oxidative stress to resist pathogen invasion. Resistance to oxidative toxicity is an important factor for pathogens. Glutathione synthesis contributes to reactive oxygen species (ROS) detoxification in bacterial cells. Little is known about the roles of glutathione synthesis and transport in S. suis. In this study, we demonstrated that glutathione treatment increased oxidative stress tolerance in S. suis. GshAB and GshT were found in S. suis glutathione synthesis and import by bioinformatics. In vitro, inactivation of gshAB and gshT led to increased sensitivity to oxidative stress. Inactivation of gshT led to growth defects in the medium. The intracellular glutathione content of gshAB or gshT deletion mutants was lower than that of wild type (WT) strain. The phagocytic resistance of gshAB and gshT mutants was lower than that of the WT strain. Moreover, the virulence of gshAB and gshT deletion mutants was significantly lower than that of the WT strain in mouse survival and tissue loading experiments. In conclusion, these results revealed the functions of GshAB and GshT in the pathogenesis of S. suis. These findings enhance our understanding of bacterial virulence mechanisms and may provide a new avenue for therapeutic intervention aimed at curbing S. suis infections. |
| format | Article |
| id | doaj-art-86e972ea94f647e08e925f2c8713fe35 |
| institution | OA Journals |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-86e972ea94f647e08e925f2c8713fe352025-08-20T02:30:10ZengTaylor & Francis GroupVirulence2150-55942150-56082025-12-0116110.1080/21505594.2025.2474866The role of glutathione for oxidative stress and pathogenicity of Streptococcus suisWei Peng0Qinggen Jiang1Yuting Wu2Li He3Bei Li4Weicheng Bei5Xia Yang6School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaSchool of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaSchool of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaSchool of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaSchool of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaNational Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, ChinaSchool of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, ChinaStreptococcus suis is an important zoonotic pathogen that threatens human and pig health. During infection, the host can impose oxidative stress to resist pathogen invasion. Resistance to oxidative toxicity is an important factor for pathogens. Glutathione synthesis contributes to reactive oxygen species (ROS) detoxification in bacterial cells. Little is known about the roles of glutathione synthesis and transport in S. suis. In this study, we demonstrated that glutathione treatment increased oxidative stress tolerance in S. suis. GshAB and GshT were found in S. suis glutathione synthesis and import by bioinformatics. In vitro, inactivation of gshAB and gshT led to increased sensitivity to oxidative stress. Inactivation of gshT led to growth defects in the medium. The intracellular glutathione content of gshAB or gshT deletion mutants was lower than that of wild type (WT) strain. The phagocytic resistance of gshAB and gshT mutants was lower than that of the WT strain. Moreover, the virulence of gshAB and gshT deletion mutants was significantly lower than that of the WT strain in mouse survival and tissue loading experiments. In conclusion, these results revealed the functions of GshAB and GshT in the pathogenesis of S. suis. These findings enhance our understanding of bacterial virulence mechanisms and may provide a new avenue for therapeutic intervention aimed at curbing S. suis infections.https://www.tandfonline.com/doi/10.1080/21505594.2025.2474866Streptococcus suisglutathioneoxidative stressGshABGshTvirulence |
| spellingShingle | Wei Peng Qinggen Jiang Yuting Wu Li He Bei Li Weicheng Bei Xia Yang The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis Virulence Streptococcus suis glutathione oxidative stress GshAB GshT virulence |
| title | The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis |
| title_full | The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis |
| title_fullStr | The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis |
| title_full_unstemmed | The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis |
| title_short | The role of glutathione for oxidative stress and pathogenicity of Streptococcus suis |
| title_sort | role of glutathione for oxidative stress and pathogenicity of streptococcus suis |
| topic | Streptococcus suis glutathione oxidative stress GshAB GshT virulence |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2025.2474866 |
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