Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats
Glyphosate, a widely used herbicide against broadleaf weeds and grasses, has been associated with various harmful effects. Our study examines the efficacy of chlorogenic acid (CGA) in alleviating the toxicity of a glyphosate-based herbicide (GBH) in 42 Wistar rats across six groups of seven animals...
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2025-03-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
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| Online Access: | https://doi.org/10.2478/aiht-2025-76-3955 |
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| author | Türkmen Ruhi Birdane Yavuz Osman Atik Orkun Demirel Hasan Huseyin Baser Durmus Fatih |
| author_facet | Türkmen Ruhi Birdane Yavuz Osman Atik Orkun Demirel Hasan Huseyin Baser Durmus Fatih |
| author_sort | Türkmen Ruhi |
| collection | DOAJ |
| description | Glyphosate, a widely used herbicide against broadleaf weeds and grasses, has been associated with various harmful effects. Our study examines the efficacy of chlorogenic acid (CGA) in alleviating the toxicity of a glyphosate-based herbicide (GBH) in 42 Wistar rats across six groups of seven animals receiving either no treatment (control), CGA alone (50 mg/kg), GBH alone (800 mg/kg), or their combinations varying three CGA doses (12.5, 25, or 50 mg/kg) (CGA12.5+GBH, CGA25+GBH, and CGA50+GBH, respectively) by oral gavage over 49 days in a row. At the end of the experiment, samples of blood, brain, heart, liver, and kidney tissues were collected and analysed for oxidative stress indicators (MDA, GSH, SOD, CAT), oxidative DNA damage (8-OHdG), liver and kidney function markers (AST, ALT, ALP, urea, and creatinine) as well as for histopathological changes. As expected, GBH increased AST ALT, ALP, urea, creatinine, 8-OHdG, and MDA levels, and lowered GSH levels and SOD and CAT activities, leaving histopathological changes in the brain, heart, liver, and kidney tissues. CGA dose-dependently improved biochemical and oxidative stress parameters and reversed histopathological changes in GBH-treated albino rats. Our findings consistently confirm the potential of CGA as a promising natural agent against the adverse health effects associated with exposure to glyphosate. Future research should focus on long-term glyphosate exposure and CGA treatment using molecular methods and on the signalling pathways associated with oxidative stress. |
| format | Article |
| id | doaj-art-86e852efd4cd45cd853e29c028bcb6c4 |
| institution | DOAJ |
| issn | 1848-6312 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj-art-86e852efd4cd45cd853e29c028bcb6c42025-08-20T03:10:17ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122025-03-01761445210.2478/aiht-2025-76-3955Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar ratsTürkmen Ruhi0Birdane Yavuz Osman1Atik Orkun2Demirel Hasan Huseyin3Baser Durmus Fatih4Afyon Kocatepe University Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyonkarahisar, TurkeyAfyon Kocatepe University Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyonkarahisar, TurkeyAfyon Kocatepe University Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology, Afyonkarahisar, TurkeyAfyon Kocatepe University Bayat Vocational School, Afyonkarahisar, TurkeyAfyon Kocatepe University Faculty of Veterinary Medicine, Department of Internal Medicine, Afyonkarahisar, TurkeyGlyphosate, a widely used herbicide against broadleaf weeds and grasses, has been associated with various harmful effects. Our study examines the efficacy of chlorogenic acid (CGA) in alleviating the toxicity of a glyphosate-based herbicide (GBH) in 42 Wistar rats across six groups of seven animals receiving either no treatment (control), CGA alone (50 mg/kg), GBH alone (800 mg/kg), or their combinations varying three CGA doses (12.5, 25, or 50 mg/kg) (CGA12.5+GBH, CGA25+GBH, and CGA50+GBH, respectively) by oral gavage over 49 days in a row. At the end of the experiment, samples of blood, brain, heart, liver, and kidney tissues were collected and analysed for oxidative stress indicators (MDA, GSH, SOD, CAT), oxidative DNA damage (8-OHdG), liver and kidney function markers (AST, ALT, ALP, urea, and creatinine) as well as for histopathological changes. As expected, GBH increased AST ALT, ALP, urea, creatinine, 8-OHdG, and MDA levels, and lowered GSH levels and SOD and CAT activities, leaving histopathological changes in the brain, heart, liver, and kidney tissues. CGA dose-dependently improved biochemical and oxidative stress parameters and reversed histopathological changes in GBH-treated albino rats. Our findings consistently confirm the potential of CGA as a promising natural agent against the adverse health effects associated with exposure to glyphosate. Future research should focus on long-term glyphosate exposure and CGA treatment using molecular methods and on the signalling pathways associated with oxidative stress.https://doi.org/10.2478/aiht-2025-76-39558-ohdgalpaltastbloodbraincatcgacreatininedna damagegshheartherbicideshistopathologykidneylivermdaoxidative stresssodurea8-ohdgalpaltastbubrezicatcgagshherbicidihistopatologijajetrakreatininkrvmdamozakoksidacijski stresoštećenje dnasodsrceurea |
| spellingShingle | Türkmen Ruhi Birdane Yavuz Osman Atik Orkun Demirel Hasan Huseyin Baser Durmus Fatih Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats Arhiv za Higijenu Rada i Toksikologiju 8-ohdg alp alt ast blood brain cat cga creatinine dna damage gsh heart herbicides histopathology kidney liver mda oxidative stress sod urea 8-ohdg alp alt ast bubrezi cat cga gsh herbicidi histopatologija jetra kreatinin krv mda mozak oksidacijski stres oštećenje dna sod srce urea |
| title | Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats |
| title_full | Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats |
| title_fullStr | Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats |
| title_full_unstemmed | Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats |
| title_short | Protective effects of chlorogenic acid against glyphosate-induced organ and blood toxicity in Wistar rats |
| title_sort | protective effects of chlorogenic acid against glyphosate induced organ and blood toxicity in wistar rats |
| topic | 8-ohdg alp alt ast blood brain cat cga creatinine dna damage gsh heart herbicides histopathology kidney liver mda oxidative stress sod urea 8-ohdg alp alt ast bubrezi cat cga gsh herbicidi histopatologija jetra kreatinin krv mda mozak oksidacijski stres oštećenje dna sod srce urea |
| url | https://doi.org/10.2478/aiht-2025-76-3955 |
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