Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm
Background Oral nitrate-reducing bacteria are associated with good oral health, with inorganic nitrate specifically promoting the growth of these beneficial bacteria. Sugar alcohols affect the composition of oral microbiota, potentially impacting oral health. The present study aimed to investigate t...
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| Format: | Article |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2025.2526069 |
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| author | Akihiko Fujii Tomoki Akatsu Hatsumi Souno Sawako Kawano Yoshihiko Minegishi Noriyasu Ota |
| author_facet | Akihiko Fujii Tomoki Akatsu Hatsumi Souno Sawako Kawano Yoshihiko Minegishi Noriyasu Ota |
| author_sort | Akihiko Fujii |
| collection | DOAJ |
| description | Background Oral nitrate-reducing bacteria are associated with good oral health, with inorganic nitrate specifically promoting the growth of these beneficial bacteria. Sugar alcohols affect the composition of oral microbiota, potentially impacting oral health. The present study aimed to investigate the combined effects of nitrate and sugar alcohols on nitrate-reducing bacteria and nitrate metabolism in oral microbiota cultured in vitro.Methods Species-level microbial analysis using 16S rRNA gene sequencing of DNA extracted from the supragingival plaque-derived biofilm cultured under micro-aerobic conditions for 48 h with nitrate and/or sugar alcohols was conducted. Nitrate metabolites, lactate, and pH in culture supernatants were also measured.Results The combined addition of nitrate and erythritol, but not xylitol or sorbitol, significantly increased the relative abundance of Haemophilus parainfluenzae and Neisseria subflava, which are nitrate-reducing bacteria. This shift was accompanied by a corresponding decrease in Streptococcus oralis, which simultaneously induced an increase in the nitrate-reducing capacity and a decrease in lactate production and acidification from sugar metabolism.Conclusions The combination of nitrate and erythritol serve as a preventive and therapeutic approach for periodontitis or dental caries by promoting the growth of oral commensal nitrate-reducing bacteria. However, human clinical studies are required to clarify these beneficial effects. |
| format | Article |
| id | doaj-art-86e2f95d63324201be21569fde3bd79e |
| institution | OA Journals |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-86e2f95d63324201be21569fde3bd79e2025-08-20T02:38:19ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2025.2526069Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilmAkihiko Fujii0Tomoki Akatsu1Hatsumi Souno2Sawako Kawano3Yoshihiko Minegishi4Noriyasu Ota5Human Health Care Products Research, Kao Corporation, Tochigi, JapanHuman Health Care Products Research, Kao Corporation, Tochigi, JapanHuman Health Care Products Research, Kao Corporation, Tochigi, JapanHuman Health Care Products Research, Kao Corporation, Tochigi, JapanHuman Health Care Products Research, Kao Corporation, Tochigi, JapanR&D Strategy, Kao Corporation, Tokyo, JapanBackground Oral nitrate-reducing bacteria are associated with good oral health, with inorganic nitrate specifically promoting the growth of these beneficial bacteria. Sugar alcohols affect the composition of oral microbiota, potentially impacting oral health. The present study aimed to investigate the combined effects of nitrate and sugar alcohols on nitrate-reducing bacteria and nitrate metabolism in oral microbiota cultured in vitro.Methods Species-level microbial analysis using 16S rRNA gene sequencing of DNA extracted from the supragingival plaque-derived biofilm cultured under micro-aerobic conditions for 48 h with nitrate and/or sugar alcohols was conducted. Nitrate metabolites, lactate, and pH in culture supernatants were also measured.Results The combined addition of nitrate and erythritol, but not xylitol or sorbitol, significantly increased the relative abundance of Haemophilus parainfluenzae and Neisseria subflava, which are nitrate-reducing bacteria. This shift was accompanied by a corresponding decrease in Streptococcus oralis, which simultaneously induced an increase in the nitrate-reducing capacity and a decrease in lactate production and acidification from sugar metabolism.Conclusions The combination of nitrate and erythritol serve as a preventive and therapeutic approach for periodontitis or dental caries by promoting the growth of oral commensal nitrate-reducing bacteria. However, human clinical studies are required to clarify these beneficial effects.https://www.tandfonline.com/doi/10.1080/20002297.2025.2526069Nitrateerythritoloral microbiotaNitrate-reducing bacteriaNeisseriaHaemophilus |
| spellingShingle | Akihiko Fujii Tomoki Akatsu Hatsumi Souno Sawako Kawano Yoshihiko Minegishi Noriyasu Ota Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm Journal of Oral Microbiology Nitrate erythritol oral microbiota Nitrate-reducing bacteria Neisseria Haemophilus |
| title | Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| title_full | Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| title_fullStr | Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| title_full_unstemmed | Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| title_short | Enhanced dominance of nitrate-reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| title_sort | enhanced dominance of nitrate reducing bacteria using a combination of nitrate and erythritol in in vitro cultured oral biofilm |
| topic | Nitrate erythritol oral microbiota Nitrate-reducing bacteria Neisseria Haemophilus |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2025.2526069 |
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