Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives
The aim of this study was to explore the effects of different lactic acid bacteria additives (<i>Lactiplantibacillus plantarum</i> or <i>Lentilactobacillus buchneri</i>) on the fermentation quality, chemical composition, in vitro digestibility, bacterial community structure,...
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2025-07-01
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| author | Yitong Jin Bao Yuan Fuhou Li Jiarui Du Meng Yu Hongyu Tang Lixia Zhang Peng Wang |
| author_facet | Yitong Jin Bao Yuan Fuhou Li Jiarui Du Meng Yu Hongyu Tang Lixia Zhang Peng Wang |
| author_sort | Yitong Jin |
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| description | The aim of this study was to explore the effects of different lactic acid bacteria additives (<i>Lactiplantibacillus plantarum</i> or <i>Lentilactobacillus buchneri</i>) on the fermentation quality, chemical composition, in vitro digestibility, bacterial community structure, and predictive function of <i>S. perfoliatum</i> silage feed. Fresh <i>S. perfoliatum</i> was wilted overnight, then its moisture content was adjusted between 65 and 70%. The experiment was performed in three groups as follows: (1) the control group (CK group), which lacked a <i>Lactobacillus</i> preparation; (2) the <i>Lactiplantibacillus plantarum</i> (<i>L. plantarum</i>) group (LP group), which was inoculated with <i>L. plantarum</i> at 5 × 10<sup>6</sup> cfu/g FW; and (3) the <i>Lentilactobacillus buchneri</i> (<i>L. buchneri</i>) group (LB group), which was inoculated with <i>L. buchneri</i> at 5 × 10<sup>6</sup> cfu/g FW. The results showed that <i>L. plantarum</i> significantly reduced pH and increased lactic acid (LA) content in <i>S. perfoliatum</i> silage compared with the control. <i>L. buchneri</i>, on the other hand, excelled in reducing ammonia nitrogen (NH<sub>3</sub>-N) content and significantly increased acetic acid (AA) content. At 60 days of fermentation, the CP content was significantly higher (<i>p</i> < 0.05) in the LP and LB groups than in the CK group (19.29 vs. 15.53 and 15.87). At 60 days of fermentation, the <i>iv</i>CPD was significantly higher (<i>p</i> < 0.05) in the LB group than in the CK and LP groups (57.80 vs. 54.77 and 55.77). The 60-day silage process completely altered the bacterial community of <i>S. perfoliatum</i> silage. In the fresh samples, the dominant genera were Weissella_A and Pantoea_A. Weissella_A and Pantoea_A were gradually replaced by <i>Lentilactobacillus</i> and <i>Lactiplantibacillus</i> after <i>S. perfoliatum</i> ensiling. After 45 days of fermentation, <i>L. buchneri</i> became the dominant strain in CK, LP and LB groups. Inoculation with <i>L. plantarum</i> altered the succession of the bacterial community from 7 to 15 days of fermentation of <i>S. perfoliatum</i>. In contrast, inoculation with <i>L. buchneri</i> affected the succession of the bacterial community from 30 to 60 days of <i>S. perfoliatum</i> fermentation. In <i>S. perfoliatum</i> silage aged 7 to 60 days, the amino acid metabolic pathway in the LB group remained upregulated. The experimental results revealed that inoculation with <i>L. buchneri</i> had a stronger effect on <i>S. perfoliatum</i> silage than inoculation with <i>L. plantarum</i>. Thus, <i>L. buchneri</i> should be selected as an additive for <i>S. perfoliatum</i> silage fermentation in practical production. |
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| issn | 2076-2615 |
| language | English |
| publishDate | 2025-07-01 |
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| series | Animals |
| spelling | doaj-art-86e2bb69649043ff94c3aa4036ee754a2025-08-20T02:35:49ZengMDPI AGAnimals2076-26152025-07-011513195510.3390/ani15131955Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria AdditivesYitong Jin0Bao Yuan1Fuhou Li2Jiarui Du3Meng Yu4Hongyu Tang5Lixia Zhang6Peng Wang7College of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaAnimal Husbandry and Veterinary Bureau of Dingxi City, Dingxi 743000, ChinaCollege of Animal Sciences, Jilin University, Changchun 130062, ChinaThe aim of this study was to explore the effects of different lactic acid bacteria additives (<i>Lactiplantibacillus plantarum</i> or <i>Lentilactobacillus buchneri</i>) on the fermentation quality, chemical composition, in vitro digestibility, bacterial community structure, and predictive function of <i>S. perfoliatum</i> silage feed. Fresh <i>S. perfoliatum</i> was wilted overnight, then its moisture content was adjusted between 65 and 70%. The experiment was performed in three groups as follows: (1) the control group (CK group), which lacked a <i>Lactobacillus</i> preparation; (2) the <i>Lactiplantibacillus plantarum</i> (<i>L. plantarum</i>) group (LP group), which was inoculated with <i>L. plantarum</i> at 5 × 10<sup>6</sup> cfu/g FW; and (3) the <i>Lentilactobacillus buchneri</i> (<i>L. buchneri</i>) group (LB group), which was inoculated with <i>L. buchneri</i> at 5 × 10<sup>6</sup> cfu/g FW. The results showed that <i>L. plantarum</i> significantly reduced pH and increased lactic acid (LA) content in <i>S. perfoliatum</i> silage compared with the control. <i>L. buchneri</i>, on the other hand, excelled in reducing ammonia nitrogen (NH<sub>3</sub>-N) content and significantly increased acetic acid (AA) content. At 60 days of fermentation, the CP content was significantly higher (<i>p</i> < 0.05) in the LP and LB groups than in the CK group (19.29 vs. 15.53 and 15.87). At 60 days of fermentation, the <i>iv</i>CPD was significantly higher (<i>p</i> < 0.05) in the LB group than in the CK and LP groups (57.80 vs. 54.77 and 55.77). The 60-day silage process completely altered the bacterial community of <i>S. perfoliatum</i> silage. In the fresh samples, the dominant genera were Weissella_A and Pantoea_A. Weissella_A and Pantoea_A were gradually replaced by <i>Lentilactobacillus</i> and <i>Lactiplantibacillus</i> after <i>S. perfoliatum</i> ensiling. After 45 days of fermentation, <i>L. buchneri</i> became the dominant strain in CK, LP and LB groups. Inoculation with <i>L. plantarum</i> altered the succession of the bacterial community from 7 to 15 days of fermentation of <i>S. perfoliatum</i>. In contrast, inoculation with <i>L. buchneri</i> affected the succession of the bacterial community from 30 to 60 days of <i>S. perfoliatum</i> fermentation. In <i>S. perfoliatum</i> silage aged 7 to 60 days, the amino acid metabolic pathway in the LB group remained upregulated. The experimental results revealed that inoculation with <i>L. buchneri</i> had a stronger effect on <i>S. perfoliatum</i> silage than inoculation with <i>L. plantarum</i>. Thus, <i>L. buchneri</i> should be selected as an additive for <i>S. perfoliatum</i> silage fermentation in practical production.https://www.mdpi.com/2076-2615/15/13/1955silageFodder storagebacterial communityfermentation qualitylactic acid bacteria additives<i>Silphium perfoliatum</i> L. |
| spellingShingle | Yitong Jin Bao Yuan Fuhou Li Jiarui Du Meng Yu Hongyu Tang Lixia Zhang Peng Wang Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives Animals silage Fodder storage bacterial community fermentation quality lactic acid bacteria additives <i>Silphium perfoliatum</i> L. |
| title | Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives |
| title_full | Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives |
| title_fullStr | Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives |
| title_full_unstemmed | Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives |
| title_short | Fermentation Characteristics, Nutrient Content, and Microbial Population of <i>Silphium perfoliatum</i> L. Silage Produced with Different Lactic Acid Bacteria Additives |
| title_sort | fermentation characteristics nutrient content and microbial population of i silphium perfoliatum i l silage produced with different lactic acid bacteria additives |
| topic | silage Fodder storage bacterial community fermentation quality lactic acid bacteria additives <i>Silphium perfoliatum</i> L. |
| url | https://www.mdpi.com/2076-2615/15/13/1955 |
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