Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus)
Plant secondary metabolites (PSMs) may improve gastrointestinal health by exerting immunomodulatory, anti-inflammatory and/or antiparasitic effects. Bark extracts from coniferous tree species have previously been shown to reduce the burden of a range of parasite species in the gastrointestinal tract...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
|
| Series: | Parasitology |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S0031182024001148/type/journal_article |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585004707741696 |
|---|---|
| author | Berit Marie Blomstrand Stig Milan Thamsborg Håvard Steinshamn Heidi Larsen Enemark Inga Marie Aasen Karl-Christian Mahnert Kristin Marie Sørheim Francesca Shepherd Jos Houdijk Spiridoula Athanasiadou |
| author_facet | Berit Marie Blomstrand Stig Milan Thamsborg Håvard Steinshamn Heidi Larsen Enemark Inga Marie Aasen Karl-Christian Mahnert Kristin Marie Sørheim Francesca Shepherd Jos Houdijk Spiridoula Athanasiadou |
| author_sort | Berit Marie Blomstrand |
| collection | DOAJ |
| description | Plant secondary metabolites (PSMs) may improve gastrointestinal health by exerting immunomodulatory, anti-inflammatory and/or antiparasitic effects. Bark extracts from coniferous tree species have previously been shown to reduce the burden of a range of parasite species in the gastrointestinal tract, with condensed tannins as the potential active compounds. In the present study, the impact of an acetone extract of pine bark (Pinus sylvestris) on the resistance, performance and tolerance of genetically diverse mice (Mus musculus) was assessed. Mice able to clear an infection quickly (fast responders, BALB/c) or slowly (slow responders, C57BL/6) were infected orally with 200 infective third-stage larvae (L3) of the parasitic nematode Heligmosomoides bakeri or remained uninfected (dosed with water only). Each infection group of mice was gavaged for 3 consecutive days from day 19 post-infection with either bark extract or dimethyl sulphoxide (5%) as vehicle control. Oral administration of pine bark extract did not have an impact on any of the measured parasitological parameter. It did, however, have a positive impact on the performance of infected, slow-responder mice, through an increase in body weight (BW) and carcase weight and reduced feed intake by BW ratio. Importantly, bark extract administration had a negative impact on the fast responders, by reducing their ability to mediate the impact of parasitism through reducing their performance and tolerance. The results indicate that the impact of PSMs on parasitized hosts is affected by host's genetic susceptibility, with susceptible hosts benefiting more from bark extract administration compared to resistant ones. |
| format | Article |
| id | doaj-art-86d8ddd6e7e04eba9ffbfea2d2adcae0 |
| institution | Kabale University |
| issn | 0031-1820 1469-8161 |
| language | English |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Parasitology |
| spelling | doaj-art-86d8ddd6e7e04eba9ffbfea2d2adcae02025-01-27T06:54:27ZengCambridge University PressParasitology0031-18201469-81611910.1017/S0031182024001148Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus)Berit Marie Blomstrand0https://orcid.org/0000-0003-1429-4305Stig Milan Thamsborg1Håvard Steinshamn2Heidi Larsen Enemark3Inga Marie Aasen4Karl-Christian Mahnert5Kristin Marie Sørheim6Francesca Shepherd7Jos Houdijk8Spiridoula Athanasiadou9https://orcid.org/0000-0002-9188-837XNorwegian Centre for Organic Agriculture, Tingvoll, NorwayDepartment of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, DenmarkDivision of Food Production and Society, Grassland and Livestock, Norwegian Institute of Bioeconomy Research, Tingvoll, NorwayDepartment of Animal Health and Food Safety, Norwegian Veterinary Institute, Oslo, Norway Department of Animal and Veterinary Sciences, Aarhus University, Tjele, DenmarkSINTEF Industry, Biotechnology and Nanomedicine, Trondheim, NorwayThe Norwegian Institute of Wood Technology, Oslo, NorwayNorwegian Centre for Organic Agriculture, Tingvoll, NorwayDepartment of Animal and Veterinary Sciences, Scotland's Rural College (SRUC), Edinburgh, UKDepartment of Animal and Veterinary Sciences, Scotland's Rural College (SRUC), Edinburgh, UKDepartment of Animal and Veterinary Sciences, Scotland's Rural College (SRUC), Edinburgh, UKPlant secondary metabolites (PSMs) may improve gastrointestinal health by exerting immunomodulatory, anti-inflammatory and/or antiparasitic effects. Bark extracts from coniferous tree species have previously been shown to reduce the burden of a range of parasite species in the gastrointestinal tract, with condensed tannins as the potential active compounds. In the present study, the impact of an acetone extract of pine bark (Pinus sylvestris) on the resistance, performance and tolerance of genetically diverse mice (Mus musculus) was assessed. Mice able to clear an infection quickly (fast responders, BALB/c) or slowly (slow responders, C57BL/6) were infected orally with 200 infective third-stage larvae (L3) of the parasitic nematode Heligmosomoides bakeri or remained uninfected (dosed with water only). Each infection group of mice was gavaged for 3 consecutive days from day 19 post-infection with either bark extract or dimethyl sulphoxide (5%) as vehicle control. Oral administration of pine bark extract did not have an impact on any of the measured parasitological parameter. It did, however, have a positive impact on the performance of infected, slow-responder mice, through an increase in body weight (BW) and carcase weight and reduced feed intake by BW ratio. Importantly, bark extract administration had a negative impact on the fast responders, by reducing their ability to mediate the impact of parasitism through reducing their performance and tolerance. The results indicate that the impact of PSMs on parasitized hosts is affected by host's genetic susceptibility, with susceptible hosts benefiting more from bark extract administration compared to resistant ones.https://www.cambridge.org/core/product/identifier/S0031182024001148/type/journal_articlebarkcondensed tanninsgastrointestinal nematodesplant secondary metabolitesresistancetolerance |
| spellingShingle | Berit Marie Blomstrand Stig Milan Thamsborg Håvard Steinshamn Heidi Larsen Enemark Inga Marie Aasen Karl-Christian Mahnert Kristin Marie Sørheim Francesca Shepherd Jos Houdijk Spiridoula Athanasiadou Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) Parasitology bark condensed tannins gastrointestinal nematodes plant secondary metabolites resistance tolerance |
| title | Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) |
| title_full | Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) |
| title_fullStr | Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) |
| title_full_unstemmed | Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) |
| title_short | Pinus sylvestris bark extract reduces the impact of Heligmosomoides bakeri infection on C57BL/6 but not on BALB/c mice (Mus musculus) |
| title_sort | pinus sylvestris bark extract reduces the impact of heligmosomoides bakeri infection on c57bl 6 but not on balb c mice mus musculus |
| topic | bark condensed tannins gastrointestinal nematodes plant secondary metabolites resistance tolerance |
| url | https://www.cambridge.org/core/product/identifier/S0031182024001148/type/journal_article |
| work_keys_str_mv | AT beritmarieblomstrand pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT stigmilanthamsborg pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT havardsteinshamn pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT heidilarsenenemark pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT ingamarieaasen pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT karlchristianmahnert pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT kristinmariesørheim pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT francescashepherd pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT joshoudijk pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus AT spiridoulaathanasiadou pinussylvestrisbarkextractreducestheimpactofheligmosomoidesbakeriinfectiononc57bl6butnotonbalbcmicemusmusculus |