Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy
Introduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with n=23 or without n=6 CMi undergoing...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/308185 |
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| author | Caroline Schmidt-Lucke Felicitas Escher Sophie Van Linthout Uwe Kühl Kapka Miteva Jochen Ringe Thomas Zobel Heinz-Peter Schultheiss Carsten Tschöpe |
| author_facet | Caroline Schmidt-Lucke Felicitas Escher Sophie Van Linthout Uwe Kühl Kapka Miteva Jochen Ringe Thomas Zobel Heinz-Peter Schultheiss Carsten Tschöpe |
| author_sort | Caroline Schmidt-Lucke |
| collection | DOAJ |
| description | Introduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with n=23 or without n=6 CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45−CD34−CD11b−CD73+CD90+ cells accounted for 0.010 [0.0025–0.048]%/peripheral mononuclear cell (PMNC) and as CD45−CD34−CD11b−CD73+CD105+ cells for 0.019 [0.0026–0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% P<0.01 transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (P<0.05, multivariate analysis). A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45−CD34−CD90+CD105+) in EMB (r=-0.73, P<0.005). SDF-1α was the strongest predictor for increased MSC in EMB (P<0.005, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation. |
| format | Article |
| id | doaj-art-86cec0ec80fb40faa1216b239f38ea43 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-86cec0ec80fb40faa1216b239f38ea432025-02-03T06:10:52ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/308185308185Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory CardiomyopathyCaroline Schmidt-Lucke0Felicitas Escher1Sophie Van Linthout2Uwe Kühl3Kapka Miteva4Jochen Ringe5Thomas Zobel6Heinz-Peter Schultheiss7Carsten Tschöpe8Department of Cardiology, Charité–University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, GermanyDepartment of Cardiology, Charité–University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, GermanyDepartment of Cardiology, Charité–University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, GermanyDepartment of Cardiology, Charité–University Medicine Berlin, Campus Rudolf Virchow, 13353 Berlin, GermanyBerlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, GermanyBerlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, GermanyDepartment of Cardiology, Charité–University Medicine Berlin, Campus Benjamin Franklin, 12200 Berlin, GermanyInstitute of Cardiac Diagnostics and Therapy (IKDT), 12203 Berlin, GermanyBerlin-Brandenburg Center of Regenerative Therapies (BCRT), 13353 Berlin, GermanyIntroduction. Mesenchymal stromal cells (MSC) have immunomodulatory features. The aim of this study was to investigate the migration and homing potential of endogenous circulating MSC in virus negative inflammatory cardiomyopathy (CMi). Methods. In 29 patients with n=23 or without n=6 CMi undergoing endomyocardial biopsies (EMB), transcardiac gradients (TCGs) of circulating MSC were measured by flow cytometry from blood simultaneously sampled from aorta and coronary sinus. The presence of MSC in EMB, cardiac inflammation, and SDF-1α mRNA expression were detected via immunohistochemistry and real-time PCR. Results. MSC defined as CD45−CD34−CD11b−CD73+CD90+ cells accounted for 0.010 [0.0025–0.048]%/peripheral mononuclear cell (PMNC) and as CD45−CD34−CD11b−CD73+CD105+ cells for 0.019 [0.0026–0.067]%/PMNC, both with similar counts in patients with or without cardiac inflammation. There was a 29.9% P<0.01 transcardiac reduction of circulating MSC in patients with CMi, correlating with the extent of cardiac inflammation (P<0.05, multivariate analysis). A strong correlation was found between the TCG of circulating MSC and numbers of MSC (CD45−CD34−CD90+CD105+) in EMB (r=-0.73, P<0.005). SDF-1α was the strongest predictor for increased MSC in EMB (P<0.005, multivariate analysis). Conclusions. Endogenous MSC continuously migrate to the heart in patients with CMi triggered by cardiac inflammation.http://dx.doi.org/10.1155/2015/308185 |
| spellingShingle | Caroline Schmidt-Lucke Felicitas Escher Sophie Van Linthout Uwe Kühl Kapka Miteva Jochen Ringe Thomas Zobel Heinz-Peter Schultheiss Carsten Tschöpe Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy Mediators of Inflammation |
| title | Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy |
| title_full | Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy |
| title_fullStr | Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy |
| title_full_unstemmed | Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy |
| title_short | Cardiac Migration of Endogenous Mesenchymal Stromal Cells in Patients with Inflammatory Cardiomyopathy |
| title_sort | cardiac migration of endogenous mesenchymal stromal cells in patients with inflammatory cardiomyopathy |
| url | http://dx.doi.org/10.1155/2015/308185 |
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