Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.

Persistence and dispersal of antibiotic resistance genes (ARGs) are important factors for assessing ARG risk in aquaculture environments. Here, we quantitatively detected ARGs for sulphonamides (sul1 and sul2) and trimethoprim (dfrA1) and an integrase gene for a class 1 integron (intI1) at aquacultu...

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Main Authors: Windi Indra Muziasari, Satoshi Managaki, Katariina Pärnänen, Antti Karkman, Christina Lyra, Manu Tamminen, Satoru Suzuki, Marko Virta
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0092702
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author Windi Indra Muziasari
Satoshi Managaki
Katariina Pärnänen
Antti Karkman
Christina Lyra
Manu Tamminen
Satoru Suzuki
Marko Virta
author_facet Windi Indra Muziasari
Satoshi Managaki
Katariina Pärnänen
Antti Karkman
Christina Lyra
Manu Tamminen
Satoru Suzuki
Marko Virta
author_sort Windi Indra Muziasari
collection DOAJ
description Persistence and dispersal of antibiotic resistance genes (ARGs) are important factors for assessing ARG risk in aquaculture environments. Here, we quantitatively detected ARGs for sulphonamides (sul1 and sul2) and trimethoprim (dfrA1) and an integrase gene for a class 1 integron (intI1) at aquaculture facilities in the northern Baltic Sea, Finland. The ARGs persisted in sediments below fish farms at very low antibiotic concentrations during the 6-year observation period from 2006 to 2012. Although the ARGs persisted in the farm sediments, they were less prevalent in the surrounding sediments. The copy numbers between the sul1 and intI1 genes were significantly correlated suggesting that class 1 integrons may play a role in the prevalence of sul1 in the farm sediments through horizontal gene transfer. In conclusion, the presence of ARGs may limit the effectiveness of antibiotics in treating fish illnesses, thereby causing a potential risk to the aquaculture industry. However, the restricted presence of ARGs at the farms is unlikely to cause serious effects in the northern Baltic Sea sediment environments around the farms.
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institution Kabale University
issn 1932-6203
language English
publishDate 2014-01-01
publisher Public Library of Science (PLoS)
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series PLoS ONE
spelling doaj-art-86ccc3336bb84d719d874ee3499415da2025-08-20T03:25:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0193e9270210.1371/journal.pone.0092702Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.Windi Indra MuziasariSatoshi ManagakiKatariina PärnänenAntti KarkmanChristina LyraManu TamminenSatoru SuzukiMarko VirtaPersistence and dispersal of antibiotic resistance genes (ARGs) are important factors for assessing ARG risk in aquaculture environments. Here, we quantitatively detected ARGs for sulphonamides (sul1 and sul2) and trimethoprim (dfrA1) and an integrase gene for a class 1 integron (intI1) at aquaculture facilities in the northern Baltic Sea, Finland. The ARGs persisted in sediments below fish farms at very low antibiotic concentrations during the 6-year observation period from 2006 to 2012. Although the ARGs persisted in the farm sediments, they were less prevalent in the surrounding sediments. The copy numbers between the sul1 and intI1 genes were significantly correlated suggesting that class 1 integrons may play a role in the prevalence of sul1 in the farm sediments through horizontal gene transfer. In conclusion, the presence of ARGs may limit the effectiveness of antibiotics in treating fish illnesses, thereby causing a potential risk to the aquaculture industry. However, the restricted presence of ARGs at the farms is unlikely to cause serious effects in the northern Baltic Sea sediment environments around the farms.https://doi.org/10.1371/journal.pone.0092702
spellingShingle Windi Indra Muziasari
Satoshi Managaki
Katariina Pärnänen
Antti Karkman
Christina Lyra
Manu Tamminen
Satoru Suzuki
Marko Virta
Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
PLoS ONE
title Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
title_full Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
title_fullStr Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
title_full_unstemmed Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
title_short Sulphonamide and trimethoprim resistance genes persist in sediments at Baltic Sea aquaculture farms but are not detected in the surrounding environment.
title_sort sulphonamide and trimethoprim resistance genes persist in sediments at baltic sea aquaculture farms but are not detected in the surrounding environment
url https://doi.org/10.1371/journal.pone.0092702
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