Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation
Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain...
Saved in:
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4937689 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849400907548065792 |
|---|---|
| author | Shuling Zhou Karolina Szczesna Anna Ochalek Julianna Kobolák Eszter Varga Csilla Nemes Abinaya Chandrasekaran Mikkel Rasmussen Susanna Cirera Poul Hyttel András Dinnyés Kristine K. Freude Hasan X. Avci |
| author_facet | Shuling Zhou Karolina Szczesna Anna Ochalek Julianna Kobolák Eszter Varga Csilla Nemes Abinaya Chandrasekaran Mikkel Rasmussen Susanna Cirera Poul Hyttel András Dinnyés Kristine K. Freude Hasan X. Avci |
| author_sort | Shuling Zhou |
| collection | DOAJ |
| description | Neural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes. |
| format | Article |
| id | doaj-art-86b694ed24f8430ca16424a6ce94d527 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-86b694ed24f8430ca16424a6ce94d5272025-08-20T03:37:53ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/49376894937689Neurosphere Based Differentiation of Human iPSC Improves Astrocyte DifferentiationShuling Zhou0Karolina Szczesna1Anna Ochalek2Julianna Kobolák3Eszter Varga4Csilla Nemes5Abinaya Chandrasekaran6Mikkel Rasmussen7Susanna Cirera8Poul Hyttel9András Dinnyés10Kristine K. Freude11Hasan X. Avci12Department of Veterinary Clinical and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkBioTalentum Ltd., Gödöllo 2100, HungaryBioTalentum Ltd., Gödöllo 2100, HungaryBioTalentum Ltd., Gödöllo 2100, HungaryBioTalentum Ltd., Gödöllo 2100, HungaryBioTalentum Ltd., Gödöllo 2100, HungaryBioTalentum Ltd., Gödöllo 2100, HungaryBioneer A/S, 2970 Hørsholm, DenmarkDepartment of Veterinary Clinical and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkDepartment of Veterinary Clinical and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkBioTalentum Ltd., Gödöllo 2100, HungaryDepartment of Veterinary Clinical and Animal Science, Faculty of Health and Medical Sciences, University of Copenhagen, 1870 Frederiksberg, DenmarkBioTalentum Ltd., Gödöllo 2100, HungaryNeural progenitor cells (NPCs) derived from human induced pluripotent stem cells (iPSCs) are traditionally maintained and proliferated utilizing two-dimensional (2D) adherent monolayer culture systems. However, NPCs cultured using this system hardly reflect the intrinsic spatial development of brain tissue. In this study, we determined that culturing iPSC-derived NPCs as three-dimensional (3D) floating neurospheres resulted in increased expression of the neural progenitor cell (NPC) markers, PAX6 and NESTIN. Expansion of NPCs in 3D culture methods also resulted in a more homogenous PAX6 expression when compared to 2D culture methods. Furthermore, the 3D propagation method for NPCs resulted in a significant higher expression of the astrocyte markers GFAP and aquaporin 4 (AQP4) in the differentiated cells. Thus, our 3D propagation method could constitute a useful tool to promote NPC homogeneity and also to increase the differentiation potential of iPSC towards astrocytes.http://dx.doi.org/10.1155/2016/4937689 |
| spellingShingle | Shuling Zhou Karolina Szczesna Anna Ochalek Julianna Kobolák Eszter Varga Csilla Nemes Abinaya Chandrasekaran Mikkel Rasmussen Susanna Cirera Poul Hyttel András Dinnyés Kristine K. Freude Hasan X. Avci Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation Stem Cells International |
| title | Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation |
| title_full | Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation |
| title_fullStr | Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation |
| title_full_unstemmed | Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation |
| title_short | Neurosphere Based Differentiation of Human iPSC Improves Astrocyte Differentiation |
| title_sort | neurosphere based differentiation of human ipsc improves astrocyte differentiation |
| url | http://dx.doi.org/10.1155/2016/4937689 |
| work_keys_str_mv | AT shulingzhou neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT karolinaszczesna neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT annaochalek neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT juliannakobolak neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT esztervarga neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT csillanemes neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT abinayachandrasekaran neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT mikkelrasmussen neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT susannacirera neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT poulhyttel neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT andrasdinnyes neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT kristinekfreude neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation AT hasanxavci neurospherebaseddifferentiationofhumanipscimprovesastrocytedifferentiation |