Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus
Forkhead box O (FOXO) transcription factors have been implicated in the development and differentiation of the immune cells. FOXO3 plays a crucial role in physiologic and pathologic immune response. FOXO3, cooperatively with FOXO1, control the development and function of Foxp3+ regulatory T cells (T...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/9484015 |
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| author | Magdalena Zurawek Marta Fichna Piotr Fichna Maria Czainska Natalia Rozwadowska |
| author_facet | Magdalena Zurawek Marta Fichna Piotr Fichna Maria Czainska Natalia Rozwadowska |
| author_sort | Magdalena Zurawek |
| collection | DOAJ |
| description | Forkhead box O (FOXO) transcription factors have been implicated in the development and differentiation of the immune cells. FOXO3 plays a crucial role in physiologic and pathologic immune response. FOXO3, cooperatively with FOXO1, control the development and function of Foxp3+ regulatory T cells (Treg). Since the lack of Treg-mediated control has fundamental impact on type 1 diabetes mellitus (T1DM) development, we investigated FOXO3 expression in patients with T1DM. FOXO3 expression was estimated in peripheral blood mononuclear cells (PBMCs) from newly diagnosed T1DM pediatric patients (n=28) and age-matched healthy donors (n=27) by reahavel-time PCR and TaqMan gene expression assays. Expression analysis revealed significant upregulation of FOXO3 in T1DM (P=0.0005). Stratification of the T1DM group according to the presence of initial diabetic ketoacidosis (DKA) did not indicate differences in FOXO3 expression in patients with DKA compared to a mild T1DM onset (P>0.05). In conclusion, overexpression of FOXO3 is correlated with the ongoing islet autoimmune destruction and might suggest a potential role for this gene in the pathogenesis of type 1 diabetes mellitus. |
| format | Article |
| id | doaj-art-86b347c079274e7ca29c045558edce97 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-86b347c079274e7ca29c045558edce972025-02-03T01:27:54ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/94840159484015Upregulation of FOXO3 in New-Onset Type 1 Diabetes MellitusMagdalena Zurawek0Marta Fichna1Piotr Fichna2Maria Czainska3Natalia Rozwadowska4Institute of Human Genetics, Polish Academy of Sciences, Poznan, PolandDepartment of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, Poznan, PolandDepartment of Paediatric Diabetes and Obesity, Poznan University of Medical Sciences, Poznan, PolandFamily Physician Clinic, Murowana Goslina, PolandInstitute of Human Genetics, Polish Academy of Sciences, Poznan, PolandForkhead box O (FOXO) transcription factors have been implicated in the development and differentiation of the immune cells. FOXO3 plays a crucial role in physiologic and pathologic immune response. FOXO3, cooperatively with FOXO1, control the development and function of Foxp3+ regulatory T cells (Treg). Since the lack of Treg-mediated control has fundamental impact on type 1 diabetes mellitus (T1DM) development, we investigated FOXO3 expression in patients with T1DM. FOXO3 expression was estimated in peripheral blood mononuclear cells (PBMCs) from newly diagnosed T1DM pediatric patients (n=28) and age-matched healthy donors (n=27) by reahavel-time PCR and TaqMan gene expression assays. Expression analysis revealed significant upregulation of FOXO3 in T1DM (P=0.0005). Stratification of the T1DM group according to the presence of initial diabetic ketoacidosis (DKA) did not indicate differences in FOXO3 expression in patients with DKA compared to a mild T1DM onset (P>0.05). In conclusion, overexpression of FOXO3 is correlated with the ongoing islet autoimmune destruction and might suggest a potential role for this gene in the pathogenesis of type 1 diabetes mellitus.http://dx.doi.org/10.1155/2020/9484015 |
| spellingShingle | Magdalena Zurawek Marta Fichna Piotr Fichna Maria Czainska Natalia Rozwadowska Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus Journal of Immunology Research |
| title | Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus |
| title_full | Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus |
| title_fullStr | Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus |
| title_full_unstemmed | Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus |
| title_short | Upregulation of FOXO3 in New-Onset Type 1 Diabetes Mellitus |
| title_sort | upregulation of foxo3 in new onset type 1 diabetes mellitus |
| url | http://dx.doi.org/10.1155/2020/9484015 |
| work_keys_str_mv | AT magdalenazurawek upregulationoffoxo3innewonsettype1diabetesmellitus AT martafichna upregulationoffoxo3innewonsettype1diabetesmellitus AT piotrfichna upregulationoffoxo3innewonsettype1diabetesmellitus AT mariaczainska upregulationoffoxo3innewonsettype1diabetesmellitus AT nataliarozwadowska upregulationoffoxo3innewonsettype1diabetesmellitus |