Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome
Changes in the BK virus archetypal noncoding control region (NCCR) have been associated with BK-virus-associated nephropathy (BKVAN). Whether sustained viremia, a surrogate for BKVAN, is associated with significant changes in the BK-NCCR is unknown. We performed PCR amplification and sequencing of (...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Journal of Transplantation |
| Online Access: | http://dx.doi.org/10.1155/2012/761283 |
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| author | Imran A. Memon Bijal A. Parikh Monique Gaudreault-Keener Rebecca Skelton Gregory A. Storch Daniel C. Brennan |
| author_facet | Imran A. Memon Bijal A. Parikh Monique Gaudreault-Keener Rebecca Skelton Gregory A. Storch Daniel C. Brennan |
| author_sort | Imran A. Memon |
| collection | DOAJ |
| description | Changes in the BK virus archetypal noncoding control region (NCCR) have been associated with BK-virus-associated nephropathy (BKVAN). Whether sustained viremia, a surrogate for BKVAN, is associated with significant changes in the BK-NCCR is unknown.
We performed PCR amplification and sequencing of (1) stored urine and (2) plasma samples from the time of peak viremia from 11 patients with sustained viremia who participated in a 200-patient clinical trial. The antimetabolite was withdrawn for BK viremia and reduction of the calcineurin inhibitor for sustained BK viremia. DNA sequencing from the 11 patients with sustained viremia revealed 8 insertions, 16 transversions, 3 deletions, and 17 transitions. None were deemed significant. No patient developed clinically evident BKVAN. Our data support, at a genomic level, the effectiveness of reduction of immunosuppression for prevention of progression from viremia to BKVAN. |
| format | Article |
| id | doaj-art-86b1ffdca58f407fbb799d79e4eb3861 |
| institution | Kabale University |
| issn | 2090-0007 2090-0015 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Transplantation |
| spelling | doaj-art-86b1ffdca58f407fbb799d79e4eb38612025-02-03T01:07:32ZengWileyJournal of Transplantation2090-00072090-00152012-01-01201210.1155/2012/761283761283Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus GenomeImran A. Memon0Bijal A. Parikh1Monique Gaudreault-Keener2Rebecca Skelton3Gregory A. Storch4Daniel C. Brennan5Department of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USABarnes-Jewish Hospital, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Pediatrics, Washington University School of Medicine, St. Louis, MO 63110, USADepartment of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USAChanges in the BK virus archetypal noncoding control region (NCCR) have been associated with BK-virus-associated nephropathy (BKVAN). Whether sustained viremia, a surrogate for BKVAN, is associated with significant changes in the BK-NCCR is unknown. We performed PCR amplification and sequencing of (1) stored urine and (2) plasma samples from the time of peak viremia from 11 patients with sustained viremia who participated in a 200-patient clinical trial. The antimetabolite was withdrawn for BK viremia and reduction of the calcineurin inhibitor for sustained BK viremia. DNA sequencing from the 11 patients with sustained viremia revealed 8 insertions, 16 transversions, 3 deletions, and 17 transitions. None were deemed significant. No patient developed clinically evident BKVAN. Our data support, at a genomic level, the effectiveness of reduction of immunosuppression for prevention of progression from viremia to BKVAN.http://dx.doi.org/10.1155/2012/761283 |
| spellingShingle | Imran A. Memon Bijal A. Parikh Monique Gaudreault-Keener Rebecca Skelton Gregory A. Storch Daniel C. Brennan Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome Journal of Transplantation |
| title | Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome |
| title_full | Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome |
| title_fullStr | Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome |
| title_full_unstemmed | Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome |
| title_short | Progression from Sustained BK Viruria to Sustained BK Viremia with Immunosuppression Reduction Is Not Associated with Changes in the Noncoding Control Region of the BK Virus Genome |
| title_sort | progression from sustained bk viruria to sustained bk viremia with immunosuppression reduction is not associated with changes in the noncoding control region of the bk virus genome |
| url | http://dx.doi.org/10.1155/2012/761283 |
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