Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats
The brain has natural antioxidant defense systems, functioning through enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase, which neutralize reactive oxygen species (ROS). Once these defense systems are overwhelmed by poisoning, oxidative damage can occur in the bra...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2025-12-01
|
| Series: | Nano TransMed |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2790676025000056 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832576419029319680 |
|---|---|
| author | Essia Hamdi Slah Hidouri Ana-Belén Muniz-Gonzalez Marwa Kechnebbou Salem Amara |
| author_facet | Essia Hamdi Slah Hidouri Ana-Belén Muniz-Gonzalez Marwa Kechnebbou Salem Amara |
| author_sort | Essia Hamdi |
| collection | DOAJ |
| description | The brain has natural antioxidant defense systems, functioning through enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase, which neutralize reactive oxygen species (ROS). Once these defense systems are overwhelmed by poisoning, oxidative damage can occur in the brain. In this study, hydrogen peroxide (H2O2) was used to induce oxidative stress. Ascorbic acid grafted to silica nanoparticles (SiO2-NPs@AA) was used as a therapeutic strategy aimed to reduce ROS levels and enhancing antioxidant defenses. The SiO2-NPs@AA were synthesized and characterized, showing efficient functionalization with ascorbic acid. The in-vitro, antioxidant assays revealed that SiO2-NPs@AA exhibited significant radical scavenging activity (DPPH and hydroxyl radicals) and high iron-chelating ability with enhanced stability compared to free ascorbic acid. Moreover, the in-vivo study demonstrated that SiO2-NPs@AA mitigated H2O2-induced effects in key enzymes, including superoxide dismutase, catalase, glutathione (GSH), and restore acetylcholinesterase (AChE) levels. Notably, malondialdehyde (MDA) levels, a marker of lipid peroxidation, were significantly reestablished in the frontal cortex and hippocampus following SiO2-NPs@AA. Overall, the study revealed that SiO2-NPs@AA corrected effectively nitric oxide (NO) and monoamine oxidase activities, which confirm their role to preserve neuronal function and mitigate neurotoxicity. |
| format | Article |
| id | doaj-art-86ac25748fd643a8a056baab6769168f |
| institution | Kabale University |
| issn | 2790-6760 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Nano TransMed |
| spelling | doaj-art-86ac25748fd643a8a056baab6769168f2025-01-31T05:12:50ZengKeAi Communications Co., Ltd.Nano TransMed2790-67602025-12-014100074Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in ratsEssia Hamdi0Slah Hidouri1Ana-Belén Muniz-Gonzalez2Marwa Kechnebbou3Salem Amara4University of Carthage, Department of Life Sciences, Faculty of Sciences of Bizerte, Tunisia; Department of Physics, Mathematics and Fluid, UNED, Av Esparta S/N, Carretera de Las Rozas al Escorial Las Rozas, Madrid 28232, Spain; Corresponding author at: University of Carthage, Department of Life Sciences, Faculty of Sciences of Bizerte, Tunisia.University of Carthage, Biochemistry and Molecular Biology Laboratory of Faculty of Sciences of Bizerte, Risks Related to Environmental Stress Prevention, Bizerte, Zarzouna UR17ES20, TunisiaDepartment of Physics, Mathematics and Fluid, UNED, Av Esparta S/N, Carretera de Las Rozas al Escorial Las Rozas, Madrid 28232, SpainDepartamento de Ecología, Universidad Autonoma de Madrid, Ciudad Universitaria de Cantoblanco, Madrid 28049, SpainUniversity of Carthage, Department of Life Sciences, Faculty of Sciences of Bizerte, Tunisia; Shaqra University College of Applied Medical Sciences, Saudi ArabiaThe brain has natural antioxidant defense systems, functioning through enzymes such as superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase, which neutralize reactive oxygen species (ROS). Once these defense systems are overwhelmed by poisoning, oxidative damage can occur in the brain. In this study, hydrogen peroxide (H2O2) was used to induce oxidative stress. Ascorbic acid grafted to silica nanoparticles (SiO2-NPs@AA) was used as a therapeutic strategy aimed to reduce ROS levels and enhancing antioxidant defenses. The SiO2-NPs@AA were synthesized and characterized, showing efficient functionalization with ascorbic acid. The in-vitro, antioxidant assays revealed that SiO2-NPs@AA exhibited significant radical scavenging activity (DPPH and hydroxyl radicals) and high iron-chelating ability with enhanced stability compared to free ascorbic acid. Moreover, the in-vivo study demonstrated that SiO2-NPs@AA mitigated H2O2-induced effects in key enzymes, including superoxide dismutase, catalase, glutathione (GSH), and restore acetylcholinesterase (AChE) levels. Notably, malondialdehyde (MDA) levels, a marker of lipid peroxidation, were significantly reestablished in the frontal cortex and hippocampus following SiO2-NPs@AA. Overall, the study revealed that SiO2-NPs@AA corrected effectively nitric oxide (NO) and monoamine oxidase activities, which confirm their role to preserve neuronal function and mitigate neurotoxicity.http://www.sciencedirect.com/science/article/pii/S2790676025000056Ascorbic acidBrain oxidative stressHydrogen peroxideNeurotoxicitySiO2-NPs |
| spellingShingle | Essia Hamdi Slah Hidouri Ana-Belén Muniz-Gonzalez Marwa Kechnebbou Salem Amara Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats Nano TransMed Ascorbic acid Brain oxidative stress Hydrogen peroxide Neurotoxicity SiO2-NPs |
| title | Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| title_full | Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| title_fullStr | Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| title_full_unstemmed | Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| title_short | Harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| title_sort | harnessing silica nanoparticles grafted with ascorbic acid to alleviate oxidative stress and impaired brain activity in rats |
| topic | Ascorbic acid Brain oxidative stress Hydrogen peroxide Neurotoxicity SiO2-NPs |
| url | http://www.sciencedirect.com/science/article/pii/S2790676025000056 |
| work_keys_str_mv | AT essiahamdi harnessingsilicananoparticlesgraftedwithascorbicacidtoalleviateoxidativestressandimpairedbrainactivityinrats AT slahhidouri harnessingsilicananoparticlesgraftedwithascorbicacidtoalleviateoxidativestressandimpairedbrainactivityinrats AT anabelenmunizgonzalez harnessingsilicananoparticlesgraftedwithascorbicacidtoalleviateoxidativestressandimpairedbrainactivityinrats AT marwakechnebbou harnessingsilicananoparticlesgraftedwithascorbicacidtoalleviateoxidativestressandimpairedbrainactivityinrats AT salemamara harnessingsilicananoparticlesgraftedwithascorbicacidtoalleviateoxidativestressandimpairedbrainactivityinrats |