Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections

Background: Drug resistance is a major problem in Escherichia coli isolated from surgical wound infections. In this study, we evaluated relationship between phylogenicity and drug resistance. Methodology: A total of 29 multi-drug resistant (MDR) E. coli isolates of known drug resistance genes and...

Full description

Saved in:
Bibliographic Details
Main Authors: Muhammad Azeem Saeed, Abdul Haque, Aamir Ali, Mashkoor Mohsin, Saira Bashir, Ayesha Tariq, Amna Afzal, Tayyaba Iftikhar, Yasra Sarwar
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2009-10-01
Series:Journal of Infection in Developing Countries
Subjects:
Online Access:https://jidc.org/index.php/journal/article/view/66
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850193045022048256
author Muhammad Azeem Saeed
Abdul Haque
Aamir Ali
Mashkoor Mohsin
Saira Bashir
Ayesha Tariq
Amna Afzal
Tayyaba Iftikhar
Yasra Sarwar
author_facet Muhammad Azeem Saeed
Abdul Haque
Aamir Ali
Mashkoor Mohsin
Saira Bashir
Ayesha Tariq
Amna Afzal
Tayyaba Iftikhar
Yasra Sarwar
author_sort Muhammad Azeem Saeed
collection DOAJ
description Background: Drug resistance is a major problem in Escherichia coli isolated from surgical wound infections. In this study, we evaluated relationship between phylogenicity and drug resistance. Methodology: A total of 29 multi-drug resistant (MDR) E. coli isolates of known drug resistance genes and integron profile were selected for the present study. Triplex PCR was conducted for phylogenetic classification of these isolates into four established phylogenetic groups: A, B1, B2 and D. Statistical analysis was done to determine the association of different drug resistance genes and integrons with the phylogenetic groups. Results: Most of the isolates (44.8%) belonged to phylogenetic group A followed by group B2 and D (24.1% each) and group B1 (6.9%). Conclusions: There is a definitive relationship between drug resistance and various phylogenetic groups of E. coli infecting wounds. A shift towards phylogenetic group A might be observed with an increasing drug resistance profile.
format Article
id doaj-art-86985c48ea204efaaa61868577e1eba0
institution OA Journals
issn 1972-2680
language English
publishDate 2009-10-01
publisher The Journal of Infection in Developing Countries
record_format Article
series Journal of Infection in Developing Countries
spelling doaj-art-86985c48ea204efaaa61868577e1eba02025-08-20T02:14:22ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-10-0130910.3855/jidc.66Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infectionsMuhammad Azeem Saeed0Abdul Haque1Aamir Ali2Mashkoor Mohsin3Saira Bashir4Ayesha Tariq5Amna Afzal6Tayyaba Iftikhar7Yasra Sarwar8Health Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, FaisalabadHealth Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), PO Box 577, Jhang Road, Faisalabad Background: Drug resistance is a major problem in Escherichia coli isolated from surgical wound infections. In this study, we evaluated relationship between phylogenicity and drug resistance. Methodology: A total of 29 multi-drug resistant (MDR) E. coli isolates of known drug resistance genes and integron profile were selected for the present study. Triplex PCR was conducted for phylogenetic classification of these isolates into four established phylogenetic groups: A, B1, B2 and D. Statistical analysis was done to determine the association of different drug resistance genes and integrons with the phylogenetic groups. Results: Most of the isolates (44.8%) belonged to phylogenetic group A followed by group B2 and D (24.1% each) and group B1 (6.9%). Conclusions: There is a definitive relationship between drug resistance and various phylogenetic groups of E. coli infecting wounds. A shift towards phylogenetic group A might be observed with an increasing drug resistance profile. https://jidc.org/index.php/journal/article/view/66Drug resistanceE. coliwoundsphylogenetic groups
spellingShingle Muhammad Azeem Saeed
Abdul Haque
Aamir Ali
Mashkoor Mohsin
Saira Bashir
Ayesha Tariq
Amna Afzal
Tayyaba Iftikhar
Yasra Sarwar
Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
Journal of Infection in Developing Countries
Drug resistance
E. coli
wounds
phylogenetic groups
title Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
title_full Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
title_fullStr Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
title_full_unstemmed Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
title_short Relationship of drug resistance to phylogenetic groups of E. coli isolates from wound infections
title_sort relationship of drug resistance to phylogenetic groups of e coli isolates from wound infections
topic Drug resistance
E. coli
wounds
phylogenetic groups
url https://jidc.org/index.php/journal/article/view/66
work_keys_str_mv AT muhammadazeemsaeed relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT abdulhaque relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT aamirali relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT mashkoormohsin relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT sairabashir relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT ayeshatariq relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT amnaafzal relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT tayyabaiftikhar relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections
AT yasrasarwar relationshipofdrugresistancetophylogeneticgroupsofecoliisolatesfromwoundinfections