Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy
Staphylococcus aureus is a pathogenic microorganism often found in animal-derived foods and is known for its ability to readily develop resistance to antibiotic treatments. This study was designed to determine the prevalence of S. aureus strains in raw milk and meat in Italy and to evaluate their an...
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Elsevier
2025-02-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X24002266 |
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| author | Debora Pinamonti Marisa Manzano Michela Maifreni Silvia Bianco Beki Domi Alessia Ferrin Jamila Anba-Mondoloni Julien Dechamps Roman Briandet Jasmina Vidic |
| author_facet | Debora Pinamonti Marisa Manzano Michela Maifreni Silvia Bianco Beki Domi Alessia Ferrin Jamila Anba-Mondoloni Julien Dechamps Roman Briandet Jasmina Vidic |
| author_sort | Debora Pinamonti |
| collection | DOAJ |
| description | Staphylococcus aureus is a pathogenic microorganism often found in animal-derived foods and is known for its ability to readily develop resistance to antibiotic treatments. This study was designed to determine the prevalence of S. aureus strains in raw milk and meat in Italy and to evaluate their antibiotic resistance profiles and biofilm production. Among the meat isolates, 41.67% were resistant to ampicillin, and 25% were methicillin-resistant S. aureus (MRSA). In milk, 20% of the isolates were resistant to gentamycin, while 5.71% were MRSA. The prevalence of multidrug-resistant strains was higher in meat (16.67%) compared to milk (5.71%). The biofilm formation capability was assessed in most of the isolates (80% in milk and 100% in meat). Representative strains exhibiting different antibiotic resistance profiles were all negative for the enterotoxin genes sea, seb, sec, sed, and see, but harbored potential virulence factors such as hemolytic activity, high pigmentation, low cell envelop permeability, charged and hydrophobicity. Finally, the interaction of representative strains with human Caco-2 intestinal cell line showed that most strains had an adhesion capacity. Our findings reveal that foodborne isolates of S. aureus present a considerable threat to consumers due to their production of virulence factors, which enhance their pathogenicity and increase the likelihood of antibiotic treatment failures. |
| format | Article |
| id | doaj-art-868d3a268eae47f2bc7e365e5f737359 |
| institution | Kabale University |
| issn | 0362-028X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj-art-868d3a268eae47f2bc7e365e5f7373592025-02-02T05:26:42ZengElsevierJournal of Food Protection0362-028X2025-02-01882100442Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern ItalyDebora Pinamonti0Marisa Manzano1Michela Maifreni2Silvia Bianco3Beki Domi4Alessia Ferrin5Jamila Anba-Mondoloni6Julien Dechamps7Roman Briandet8Jasmina Vidic9University of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, ItalyUniversity of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, Italy; Corresponding authors.University of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, ItalyUniversity of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, ItalyUniversity of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, ItalyUniversity of Udine, Department of Agricultural, Food, Environmental and Animal Science, 33100 Udine, ItalyUniversité Paris-Saclay, Micalis Institute, INRAE, AgroParisTech, 78352 Jouy en Josas, FranceUniversité Paris-Saclay, Micalis Institute, INRAE, AgroParisTech, 78352 Jouy en Josas, FranceUniversité Paris-Saclay, Micalis Institute, INRAE, AgroParisTech, 78352 Jouy en Josas, FranceUniversité Paris-Saclay, Micalis Institute, INRAE, AgroParisTech, 78352 Jouy en Josas, France; Corresponding authors.Staphylococcus aureus is a pathogenic microorganism often found in animal-derived foods and is known for its ability to readily develop resistance to antibiotic treatments. This study was designed to determine the prevalence of S. aureus strains in raw milk and meat in Italy and to evaluate their antibiotic resistance profiles and biofilm production. Among the meat isolates, 41.67% were resistant to ampicillin, and 25% were methicillin-resistant S. aureus (MRSA). In milk, 20% of the isolates were resistant to gentamycin, while 5.71% were MRSA. The prevalence of multidrug-resistant strains was higher in meat (16.67%) compared to milk (5.71%). The biofilm formation capability was assessed in most of the isolates (80% in milk and 100% in meat). Representative strains exhibiting different antibiotic resistance profiles were all negative for the enterotoxin genes sea, seb, sec, sed, and see, but harbored potential virulence factors such as hemolytic activity, high pigmentation, low cell envelop permeability, charged and hydrophobicity. Finally, the interaction of representative strains with human Caco-2 intestinal cell line showed that most strains had an adhesion capacity. Our findings reveal that foodborne isolates of S. aureus present a considerable threat to consumers due to their production of virulence factors, which enhance their pathogenicity and increase the likelihood of antibiotic treatment failures.http://www.sciencedirect.com/science/article/pii/S0362028X24002266Antimicrobial resistanceBacterial cell surfaceBiofilm production capabilityCaco-2 cell lineFoodStaphylococcus aureus |
| spellingShingle | Debora Pinamonti Marisa Manzano Michela Maifreni Silvia Bianco Beki Domi Alessia Ferrin Jamila Anba-Mondoloni Julien Dechamps Roman Briandet Jasmina Vidic Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy Journal of Food Protection Antimicrobial resistance Bacterial cell surface Biofilm production capability Caco-2 cell line Food Staphylococcus aureus |
| title | Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy |
| title_full | Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy |
| title_fullStr | Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy |
| title_full_unstemmed | Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy |
| title_short | Prevalence and Characterization of Staphylococcus aureus Isolated from Meat and Milk in Northeastern Italy |
| title_sort | prevalence and characterization of staphylococcus aureus isolated from meat and milk in northeastern italy |
| topic | Antimicrobial resistance Bacterial cell surface Biofilm production capability Caco-2 cell line Food Staphylococcus aureus |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X24002266 |
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