A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception

The precise determination of leaf shape is crucial for the quantification of morphological variations between individual leaf ranks and cultivars and simulating their impact on light interception in functional-structural plant models (FSPMs). Standard manual measurements on destructively collected l...

Full description

Saved in:
Bibliographic Details
Main Authors: Dina Otto, Sebastian Munz, Emir Memic, Jens Hartung, Simone Graeff-Hönninger
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-06-01
Series:Frontiers in Plant Science
Subjects:
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1521242/full
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849329292324896768
author Dina Otto
Sebastian Munz
Emir Memic
Jens Hartung
Simone Graeff-Hönninger
author_facet Dina Otto
Sebastian Munz
Emir Memic
Jens Hartung
Simone Graeff-Hönninger
author_sort Dina Otto
collection DOAJ
description The precise determination of leaf shape is crucial for the quantification of morphological variations between individual leaf ranks and cultivars and simulating their impact on light interception in functional-structural plant models (FSPMs). Standard manual measurements on destructively collected leaves are time-intensive and prone to errors, particularly in maize (Zea mays L.), which has large, undulating leaves that are difficult to flatten. To overcome these limitations, this study presents a new camera method developed as an image-based computer vision approach method for maize leaf shape analysis. A field experiment was conducted with seven commonly used silage maize cultivars at the experimental station Heidfeldhof, University of Hohenheim, Germany, in 2022. To determine the dimensions of fully developed leaves per rank and cultivar, three destructive measurements were conducted until flowering. The new camera method employs a GoPro Hero8 Black camera, integrated within an LI-3100C Area Meter, to capture high-resolution videos (1920 × 1080 pixels, 60 fps). A semi-automated software facilitates object detection, contour extraction, and leaf width determination, including calibration for accuracy. Validation was performed using pixel-counting and contrast analysis, comparing results against standard manual measurements to assess accuracy and reliability. Leaf width functions were fitted to quantify leaf shape parameters. Statistical analysis comparing cultivars and leaf ranks identified significant differences in leaf shape parameters (p < 0.01) for term alpha and term a. Simulations within a FSPM demonstrated that variations in leaf shape can alter light interception by up to 7%, emphasizing the need for precise parameterization in crop growth models. The new camera method provides a basis for future studies investigating rank-dependent leaf shape effects, which can offer an accurate representation of the canopy in FSPMs and improve agricultural decision-making.
format Article
id doaj-art-866ce8ca118d4e70a87750a124fb3301
institution Kabale University
issn 1664-462X
language English
publishDate 2025-06-01
publisher Frontiers Media S.A.
record_format Article
series Frontiers in Plant Science
spelling doaj-art-866ce8ca118d4e70a87750a124fb33012025-08-20T03:47:19ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-06-011610.3389/fpls.2025.15212421521242A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interceptionDina Otto0Sebastian Munz1Emir Memic2Jens Hartung3Simone Graeff-Hönninger4Institute of Crop Science, Agronomy Department, University of Hohenheim, Stuttgart, GermanyInstitute of Crop Science, Agronomy Department, University of Hohenheim, Stuttgart, GermanyInstitute of Crop Science, Agronomy Department, University of Hohenheim, Stuttgart, GermanyDepartment Sustainable Agriculture and Energy Systems, University of Applied Science, Freising, GermanyInstitute of Crop Science, Agronomy Department, University of Hohenheim, Stuttgart, GermanyThe precise determination of leaf shape is crucial for the quantification of morphological variations between individual leaf ranks and cultivars and simulating their impact on light interception in functional-structural plant models (FSPMs). Standard manual measurements on destructively collected leaves are time-intensive and prone to errors, particularly in maize (Zea mays L.), which has large, undulating leaves that are difficult to flatten. To overcome these limitations, this study presents a new camera method developed as an image-based computer vision approach method for maize leaf shape analysis. A field experiment was conducted with seven commonly used silage maize cultivars at the experimental station Heidfeldhof, University of Hohenheim, Germany, in 2022. To determine the dimensions of fully developed leaves per rank and cultivar, three destructive measurements were conducted until flowering. The new camera method employs a GoPro Hero8 Black camera, integrated within an LI-3100C Area Meter, to capture high-resolution videos (1920 × 1080 pixels, 60 fps). A semi-automated software facilitates object detection, contour extraction, and leaf width determination, including calibration for accuracy. Validation was performed using pixel-counting and contrast analysis, comparing results against standard manual measurements to assess accuracy and reliability. Leaf width functions were fitted to quantify leaf shape parameters. Statistical analysis comparing cultivars and leaf ranks identified significant differences in leaf shape parameters (p < 0.01) for term alpha and term a. Simulations within a FSPM demonstrated that variations in leaf shape can alter light interception by up to 7%, emphasizing the need for precise parameterization in crop growth models. The new camera method provides a basis for future studies investigating rank-dependent leaf shape effects, which can offer an accurate representation of the canopy in FSPMs and improve agricultural decision-making.https://www.frontiersin.org/articles/10.3389/fpls.2025.1521242/fullleaf shapeleaf widthmaize (Zea mays L.)computer visionFSPMlight interception
spellingShingle Dina Otto
Sebastian Munz
Emir Memic
Jens Hartung
Simone Graeff-Hönninger
A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
Frontiers in Plant Science
leaf shape
leaf width
maize (Zea mays L.)
computer vision
FSPM
light interception
title A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
title_full A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
title_fullStr A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
title_full_unstemmed A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
title_short A computer vision approach for quantifying leaf shape of maize (Zea mays L.) and simulating its impact on light interception
title_sort computer vision approach for quantifying leaf shape of maize zea mays l and simulating its impact on light interception
topic leaf shape
leaf width
maize (Zea mays L.)
computer vision
FSPM
light interception
url https://www.frontiersin.org/articles/10.3389/fpls.2025.1521242/full
work_keys_str_mv AT dinaotto acomputervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT sebastianmunz acomputervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT emirmemic acomputervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT jenshartung acomputervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT simonegraeffhonninger acomputervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT dinaotto computervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT sebastianmunz computervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT emirmemic computervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT jenshartung computervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception
AT simonegraeffhonninger computervisionapproachforquantifyingleafshapeofmaizezeamayslandsimulatingitsimpactonlightinterception