From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry
The tumor microenvironment (TME) is a complex ecosystem where malignant and non-malignant cells cooperate and interact determining cancer progression. Cell abundance, phenotype and localization within the TME vary over tumor development and in response to therapeutic interventions. Therefore, increa...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1544844/full |
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| author | Marco Erreni Marco Erreni Maria Rita Fumagalli Matteo Marozzi Roberto Leone Raffaella Parente Raffaella D’Anna Andrea Doni |
| author_facet | Marco Erreni Marco Erreni Maria Rita Fumagalli Matteo Marozzi Roberto Leone Raffaella Parente Raffaella D’Anna Andrea Doni |
| author_sort | Marco Erreni |
| collection | DOAJ |
| description | The tumor microenvironment (TME) is a complex ecosystem where malignant and non-malignant cells cooperate and interact determining cancer progression. Cell abundance, phenotype and localization within the TME vary over tumor development and in response to therapeutic interventions. Therefore, increasing our knowledge of the spatiotemporal changes in the tumor ecosystem architecture is of importance to better understand the etiologic development of the neoplastic diseases. Imaging Mass Cytometry (IMC) represents the elective multiplexed imaging technology enabling the in-situ analysis of up to 43 different protein markers for in-depth phenotypic and spatial investigation of cells in their preserved microenvironment. IMC is currently applied in cancer research to define the composition of the cellular landscape and to identify biomarkers of predictive and prognostic significance with relevance in mechanisms of drug resistance. Herein, we describe the general principles and experimental workflow of IMC raising the informative potential in preclinical and clinical cancer research. |
| format | Article |
| id | doaj-art-86505411e1be4f64ab34c771bc7dbbc2 |
| institution | DOAJ |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-86505411e1be4f64ab34c771bc7dbbc22025-08-20T03:09:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-04-011610.3389/fimmu.2025.15448441544844From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometryMarco Erreni0Marco Erreni1Maria Rita Fumagalli2Matteo Marozzi3Roberto Leone4Raffaella Parente5Raffaella D’Anna6Andrea Doni7Unit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyDepartment of Biomedical Sciences, Humanitas University, Pieve Emanuele, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyUnit of Multiscale and Nanostructural Imaging, IRCCS Humanitas Research Hospital, Rozzano, Milan, ItalyThe tumor microenvironment (TME) is a complex ecosystem where malignant and non-malignant cells cooperate and interact determining cancer progression. Cell abundance, phenotype and localization within the TME vary over tumor development and in response to therapeutic interventions. Therefore, increasing our knowledge of the spatiotemporal changes in the tumor ecosystem architecture is of importance to better understand the etiologic development of the neoplastic diseases. Imaging Mass Cytometry (IMC) represents the elective multiplexed imaging technology enabling the in-situ analysis of up to 43 different protein markers for in-depth phenotypic and spatial investigation of cells in their preserved microenvironment. IMC is currently applied in cancer research to define the composition of the cellular landscape and to identify biomarkers of predictive and prognostic significance with relevance in mechanisms of drug resistance. Herein, we describe the general principles and experimental workflow of IMC raising the informative potential in preclinical and clinical cancer research.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1544844/fullimaging mass cytometrytumor microenvironmentmultiplexed histopathologycancerpancreatic cancer |
| spellingShingle | Marco Erreni Marco Erreni Maria Rita Fumagalli Matteo Marozzi Roberto Leone Raffaella Parente Raffaella D’Anna Andrea Doni From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry Frontiers in Immunology imaging mass cytometry tumor microenvironment multiplexed histopathology cancer pancreatic cancer |
| title | From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| title_full | From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| title_fullStr | From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| title_full_unstemmed | From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| title_short | From surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| title_sort | from surfing to diving into the tumor microenvironment through multiparametric imaging mass cytometry |
| topic | imaging mass cytometry tumor microenvironment multiplexed histopathology cancer pancreatic cancer |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1544844/full |
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