Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste
This study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP)....
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1660-3397/23/3/122 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850205065226223616 |
|---|---|
| author | Narjes Badfar Ali Jafarpour Federico Casanova Lucas Sales Queiroz Adane Tilahun Getachew Charlotte Jacobsen Flemming Jessen Nina Gringer |
| author_facet | Narjes Badfar Ali Jafarpour Federico Casanova Lucas Sales Queiroz Adane Tilahun Getachew Charlotte Jacobsen Flemming Jessen Nina Gringer |
| author_sort | Narjes Badfar |
| collection | DOAJ |
| description | This study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP). ALC-treated PC achieved the highest protein recovery (63.49%), extraction yield (24.73%), and hydrolysis degree (18.10%) (<i>p</i> < 0.05). SFE-treated hydrolysates showed higher zeta potential (−47.23 to −49.93 mV) than non-SFE samples (−25.15 to −38.62 mV) but had larger droplet sizes, indicating lower emulsion stability. SC-ALC displayed reduced fluorescence intensity and a red shift in maximum wavelength. TRYP hydrolysates reduced interfacial tension (20 mN/m), similar to sodium caseinate (Na-Cas, 13 mN/m), but with lesser effects. Dilatational rheology showed TRYP hydrolysates formed stronger, solid-like structures. These results emphasize protease efficacy over SFE for extracting functional compounds, enhancing shrimp waste valorization. |
| format | Article |
| id | doaj-art-864aa24d9ec04d2f9282ffc7e3871774 |
| institution | OA Journals |
| issn | 1660-3397 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj-art-864aa24d9ec04d2f9282ffc7e38717742025-08-20T02:11:11ZengMDPI AGMarine Drugs1660-33972025-03-0123312210.3390/md23030122Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp WasteNarjes Badfar0Ali Jafarpour1Federico Casanova2Lucas Sales Queiroz3Adane Tilahun Getachew4Charlotte Jacobsen5Flemming Jessen6Nina Gringer7Research Group for Bioactives—Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Bioactives—Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Food Production Engineering, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Bioactives—Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Bioactives—Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Bioactives—Analysis and Application, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Food Production Engineering, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkResearch Group for Food Production Engineering, Division of Food Technology, National Food Institute, Technical University of Denmark, DK-2800 Copenhagen, DenmarkThis study explored how combining supercritical fluid extraction (SFE) and enzymatic hydrolysis influences the structure and functionality of peptides recovered from filter-pressed shrimp waste. Freeze-dried press cake (PC) was defatted via SFE and hydrolyzed using Alcalase (ALC) and trypsin (TRYP). ALC-treated PC achieved the highest protein recovery (63.49%), extraction yield (24.73%), and hydrolysis degree (18.10%) (<i>p</i> < 0.05). SFE-treated hydrolysates showed higher zeta potential (−47.23 to −49.93 mV) than non-SFE samples (−25.15 to −38.62 mV) but had larger droplet sizes, indicating lower emulsion stability. SC-ALC displayed reduced fluorescence intensity and a red shift in maximum wavelength. TRYP hydrolysates reduced interfacial tension (20 mN/m), similar to sodium caseinate (Na-Cas, 13 mN/m), but with lesser effects. Dilatational rheology showed TRYP hydrolysates formed stronger, solid-like structures. These results emphasize protease efficacy over SFE for extracting functional compounds, enhancing shrimp waste valorization.https://www.mdpi.com/1660-3397/23/3/122enzymatic hydrolysissupercritical fluid extractioninterfacial rheology |
| spellingShingle | Narjes Badfar Ali Jafarpour Federico Casanova Lucas Sales Queiroz Adane Tilahun Getachew Charlotte Jacobsen Flemming Jessen Nina Gringer Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste Marine Drugs enzymatic hydrolysis supercritical fluid extraction interfacial rheology |
| title | Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste |
| title_full | Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste |
| title_fullStr | Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste |
| title_full_unstemmed | Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste |
| title_short | Influence of Supercritical Fluid Extraction Process on Techno-Functionality of Enzymatically Derived Peptides from Filter-Pressed Shrimp Waste |
| title_sort | influence of supercritical fluid extraction process on techno functionality of enzymatically derived peptides from filter pressed shrimp waste |
| topic | enzymatic hydrolysis supercritical fluid extraction interfacial rheology |
| url | https://www.mdpi.com/1660-3397/23/3/122 |
| work_keys_str_mv | AT narjesbadfar influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT alijafarpour influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT federicocasanova influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT lucassalesqueiroz influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT adanetilahungetachew influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT charlottejacobsen influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT flemmingjessen influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste AT ninagringer influenceofsupercriticalfluidextractionprocessontechnofunctionalityofenzymaticallyderivedpeptidesfromfilterpressedshrimpwaste |