Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria
Archaea have been identified as early colonizers of the human intestine, appearing from the first days of life. It is hypothesized that the origin of many of these archaea is through vertical transmission during breastfeeding. In this study, we aimed to characterize the archaeal composition in sampl...
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MDPI AG
2025-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/13/1/85 |
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| author | Maricarmen Salas-López Juan Manuel Vélez-Ixta Diana Laura Rojas-Guerrero Alberto Piña-Escobedo José Manuel Hernández-Hernández Martín Noé Rangel-Calvillo Claudia Pérez-Cruz Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Jaime García-Mena |
| author_facet | Maricarmen Salas-López Juan Manuel Vélez-Ixta Diana Laura Rojas-Guerrero Alberto Piña-Escobedo José Manuel Hernández-Hernández Martín Noé Rangel-Calvillo Claudia Pérez-Cruz Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Jaime García-Mena |
| author_sort | Maricarmen Salas-López |
| collection | DOAJ |
| description | Archaea have been identified as early colonizers of the human intestine, appearing from the first days of life. It is hypothesized that the origin of many of these archaea is through vertical transmission during breastfeeding. In this study, we aimed to characterize the archaeal composition in samples of mother-neonate pairs to observe the potential vertical transmission. We performed a cross-sectional study characterizing the archaeal diversity of 40 human colostrum-neonatal stool samples by next-generation sequencing of V5–V6 16S rDNA libraries. Intra- and inter-sample analyses were carried out to describe the Archaeal diversity in each sample type. Human colostrum and neonatal stools presented similar core microbiota, mainly composed of the methanogens <i>Methanoculleus</i> and <i>Methanosarcina</i>. Beta diversity and metabolic prediction results suggest homogeneity between sample types. Further, the co-occurrence network analysis showed associations between Archaea and Bacteria, which might be relevant for these organisms’ presence in the human milk and neonatal stool ecosystems. According to relative abundance proportions, beta diversity, and co-occurrence analyses, the similarities found imply that there is vertical transmission of archaea through breastfeeding. Nonetheless, differential abundances between the sample types suggest other relevant sources for colonizing archaea to the neonatal gut. |
| format | Article |
| id | doaj-art-864563c627874fd8abe672fba6aec626 |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj-art-864563c627874fd8abe672fba6aec6262025-01-24T13:42:35ZengMDPI AGMicroorganisms2076-26072025-01-011318510.3390/microorganisms13010085Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with BacteriaMaricarmen Salas-López0Juan Manuel Vélez-Ixta1Diana Laura Rojas-Guerrero2Alberto Piña-Escobedo3José Manuel Hernández-Hernández4Martín Noé Rangel-Calvillo5Claudia Pérez-Cruz6Karina Corona-Cervantes7Carmen Josefina Juárez-Castelán8Jaime García-Mena9Departamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoHospital General “Dr. José María Rodríguez”, Ecatepec de Morelos 55200, MexicoDepartamento de Farmacología, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoDepartamento de Genética y Biología Molecular, Cinvestav, Av. Instituto Politécnico Nacional 2508, Mexico City 07360, MexicoArchaea have been identified as early colonizers of the human intestine, appearing from the first days of life. It is hypothesized that the origin of many of these archaea is through vertical transmission during breastfeeding. In this study, we aimed to characterize the archaeal composition in samples of mother-neonate pairs to observe the potential vertical transmission. We performed a cross-sectional study characterizing the archaeal diversity of 40 human colostrum-neonatal stool samples by next-generation sequencing of V5–V6 16S rDNA libraries. Intra- and inter-sample analyses were carried out to describe the Archaeal diversity in each sample type. Human colostrum and neonatal stools presented similar core microbiota, mainly composed of the methanogens <i>Methanoculleus</i> and <i>Methanosarcina</i>. Beta diversity and metabolic prediction results suggest homogeneity between sample types. Further, the co-occurrence network analysis showed associations between Archaea and Bacteria, which might be relevant for these organisms’ presence in the human milk and neonatal stool ecosystems. According to relative abundance proportions, beta diversity, and co-occurrence analyses, the similarities found imply that there is vertical transmission of archaea through breastfeeding. Nonetheless, differential abundances between the sample types suggest other relevant sources for colonizing archaea to the neonatal gut.https://www.mdpi.com/2076-2607/13/1/85breastfeedinghuman milkmicrobiotaArchaeaneonatal gut16S rDNA |
| spellingShingle | Maricarmen Salas-López Juan Manuel Vélez-Ixta Diana Laura Rojas-Guerrero Alberto Piña-Escobedo José Manuel Hernández-Hernández Martín Noé Rangel-Calvillo Claudia Pérez-Cruz Karina Corona-Cervantes Carmen Josefina Juárez-Castelán Jaime García-Mena Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria Microorganisms breastfeeding human milk microbiota Archaea neonatal gut 16S rDNA |
| title | Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria |
| title_full | Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria |
| title_fullStr | Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria |
| title_full_unstemmed | Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria |
| title_short | Human Milk Archaea Associated with Neonatal Gut Colonization and Its Co-Occurrence with Bacteria |
| title_sort | human milk archaea associated with neonatal gut colonization and its co occurrence with bacteria |
| topic | breastfeeding human milk microbiota Archaea neonatal gut 16S rDNA |
| url | https://www.mdpi.com/2076-2607/13/1/85 |
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