G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner

Objective. It has been increasingly appreciated that G protein-coupled estrogen receptor 1 (GPER1) mediates both proinflammatory and anti-inflammatory response of estrogen. It is also involved in some rapid vascular effects of aldosterone in a mineralocorticoid receptor (MR) independent manner. Howe...

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Main Authors: Ziwei Tang, Qifu Li, Qingfeng Cheng, Mei Mei, Ying Song, Zhipeng Du, Wenwen He, Jinbo Hu, Shumin Yang, Zhihong Wang
Format: Article
Language:English
Published: Wiley 2021-01-01
Series:International Journal of Endocrinology
Online Access:http://dx.doi.org/10.1155/2021/5575927
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author Ziwei Tang
Qifu Li
Qingfeng Cheng
Mei Mei
Ying Song
Zhipeng Du
Wenwen He
Jinbo Hu
Shumin Yang
Zhihong Wang
author_facet Ziwei Tang
Qifu Li
Qingfeng Cheng
Mei Mei
Ying Song
Zhipeng Du
Wenwen He
Jinbo Hu
Shumin Yang
Zhihong Wang
author_sort Ziwei Tang
collection DOAJ
description Objective. It has been increasingly appreciated that G protein-coupled estrogen receptor 1 (GPER1) mediates both proinflammatory and anti-inflammatory response of estrogen. It is also involved in some rapid vascular effects of aldosterone in a mineralocorticoid receptor (MR) independent manner. However, whether GPER1 mediates aldosterone-induced inflammation response in endothelial cells and its relationship with MR are yet undetermined and therefore require further explanation. Method. Based on the hypothesis that GPER1 plays a role in the aldosterone-related vascular inflammation, the present study utilized a model of human umbilical vein endothelial cells transfected with MR siRNA and induced for inflammatory response with increasing concentration of aldosterone. Results. It was discovered that induction of aldosterone had no effect on the expression of GPER1 but promoted the expression of MR. Suppression of MR did not influence GPER1 expression, and GPER1 was capable of mediating part of aldosterone-induced endothelial inflammatory response. This effect may involve phosphoinositide 3-kinases (PI3K) pathway signaling. Conclusion. These findings not only demonstrated the role of GPER1 in aldosterone-induced vascular inflammation but also suggested an alternative for pharmaceutical treatment of hyperaldosteronism considering the unsatisfying effect on cardiovascular risks with MR antagonists.
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publishDate 2021-01-01
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spelling doaj-art-863e804300be484cab8f8c85ec9d6ba52025-08-20T02:08:47ZengWileyInternational Journal of Endocrinology1687-83371687-83452021-01-01202110.1155/2021/55759275575927G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent MannerZiwei Tang0Qifu Li1Qingfeng Cheng2Mei Mei3Ying Song4Zhipeng Du5Wenwen He6Jinbo Hu7Shumin Yang8Zhihong Wang9Department of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400044, ChinaObjective. It has been increasingly appreciated that G protein-coupled estrogen receptor 1 (GPER1) mediates both proinflammatory and anti-inflammatory response of estrogen. It is also involved in some rapid vascular effects of aldosterone in a mineralocorticoid receptor (MR) independent manner. However, whether GPER1 mediates aldosterone-induced inflammation response in endothelial cells and its relationship with MR are yet undetermined and therefore require further explanation. Method. Based on the hypothesis that GPER1 plays a role in the aldosterone-related vascular inflammation, the present study utilized a model of human umbilical vein endothelial cells transfected with MR siRNA and induced for inflammatory response with increasing concentration of aldosterone. Results. It was discovered that induction of aldosterone had no effect on the expression of GPER1 but promoted the expression of MR. Suppression of MR did not influence GPER1 expression, and GPER1 was capable of mediating part of aldosterone-induced endothelial inflammatory response. This effect may involve phosphoinositide 3-kinases (PI3K) pathway signaling. Conclusion. These findings not only demonstrated the role of GPER1 in aldosterone-induced vascular inflammation but also suggested an alternative for pharmaceutical treatment of hyperaldosteronism considering the unsatisfying effect on cardiovascular risks with MR antagonists.http://dx.doi.org/10.1155/2021/5575927
spellingShingle Ziwei Tang
Qifu Li
Qingfeng Cheng
Mei Mei
Ying Song
Zhipeng Du
Wenwen He
Jinbo Hu
Shumin Yang
Zhihong Wang
G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
International Journal of Endocrinology
title G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
title_full G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
title_fullStr G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
title_full_unstemmed G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
title_short G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
title_sort g protein coupled estrogen receptor 1 gper1 mediates aldosterone induced endothelial inflammation in a mineralocorticoid receptor independent manner
url http://dx.doi.org/10.1155/2021/5575927
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