In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes
Introduction. Xylopic acid (XA), the major constituent of the fruit of Xylopia aethiopica, has shown several pharmacological properties. Traditionally, the plant is used to treat several diseases and is being used in the preparation of several local foods despite the lack of information about its sa...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Advances in Pharmacological and Pharmaceutical Sciences |
| Online Access: | http://dx.doi.org/10.1155/2022/4524877 |
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| author | Mary A. Agbenyeku Regina Appiah-Opong Ernest Obese Robert P. Biney Emmanuel A. Adakudugu Arnold D. Forkuo Silas A. Osei Mustapha K. Abeka Elvis O. Ameyaw |
| author_facet | Mary A. Agbenyeku Regina Appiah-Opong Ernest Obese Robert P. Biney Emmanuel A. Adakudugu Arnold D. Forkuo Silas A. Osei Mustapha K. Abeka Elvis O. Ameyaw |
| author_sort | Mary A. Agbenyeku |
| collection | DOAJ |
| description | Introduction. Xylopic acid (XA), the major constituent of the fruit of Xylopia aethiopica, has shown several pharmacological properties. Traditionally, the plant is used to treat several diseases and is being used in the preparation of several local foods despite the lack of information about its safety, food-drug interaction, and other pharmacokinetic properties. This study, therefore, investigated the effect of XA on rat liver cytochrome P450 (CYP) enzymes in vivo and in vitro. Methods. Inhibition or induction of some isoforms of CYP450 enzymes: CYP 1A1/1A2, 1A2, 2B1/2B2, 3A4, 2D6, and 2C9 were investigated using microsomal fractions of the liver obtained from rats pretreated with a low dose of xylopic acid (LDT) 30 mg/kg, high dose of xylopic acid (HDT) 100 mg/kg, phenobarbitone (PC) 80 mg/kg, and ketoconazole (NC) 100 mg/kg, and a no-treatment group received distilled water, with (n = 5) animals in each group. The in vitro inhibition of CYP 3A4 was assessed by treating rat liver microsomes with XA. Results. Xylopic acid induced CYP 1A1/1A2, 1A2, 2D6, and 2C9, inhibited CYP 3A4, and had no effect on 2B1/2B2. Conclusion. The findings would help mitigate toxicity and therapeutic failure especially in cases of coadministration of medications with food containing XA, with metabolism altered by the latter. |
| format | Article |
| id | doaj-art-86374c232d8b4086a462e1cf4ff4face |
| institution | Kabale University |
| issn | 2633-4690 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological and Pharmaceutical Sciences |
| spelling | doaj-art-86374c232d8b4086a462e1cf4ff4face2025-08-20T03:38:05ZengWileyAdvances in Pharmacological and Pharmaceutical Sciences2633-46902022-01-01202210.1155/2022/4524877In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 EnzymesMary A. Agbenyeku0Regina Appiah-Opong1Ernest Obese2Robert P. Biney3Emmanuel A. Adakudugu4Arnold D. Forkuo5Silas A. Osei6Mustapha K. Abeka7Elvis O. Ameyaw8Noguchi Memorial Institute for Medical ResearchNoguchi Memorial Institute for Medical ResearchSchool of Pharmacy and Pharmaceutical SciencesSchool of Pharmacy and Pharmaceutical SciencesSchool of Pharmacy and Pharmaceutical SciencesFaculty of Pharmacy and Pharmaceutical SciencesDepartment of Biomedical ScienceSchool of Pharmacy and Pharmaceutical SciencesSchool of Pharmacy and Pharmaceutical SciencesIntroduction. Xylopic acid (XA), the major constituent of the fruit of Xylopia aethiopica, has shown several pharmacological properties. Traditionally, the plant is used to treat several diseases and is being used in the preparation of several local foods despite the lack of information about its safety, food-drug interaction, and other pharmacokinetic properties. This study, therefore, investigated the effect of XA on rat liver cytochrome P450 (CYP) enzymes in vivo and in vitro. Methods. Inhibition or induction of some isoforms of CYP450 enzymes: CYP 1A1/1A2, 1A2, 2B1/2B2, 3A4, 2D6, and 2C9 were investigated using microsomal fractions of the liver obtained from rats pretreated with a low dose of xylopic acid (LDT) 30 mg/kg, high dose of xylopic acid (HDT) 100 mg/kg, phenobarbitone (PC) 80 mg/kg, and ketoconazole (NC) 100 mg/kg, and a no-treatment group received distilled water, with (n = 5) animals in each group. The in vitro inhibition of CYP 3A4 was assessed by treating rat liver microsomes with XA. Results. Xylopic acid induced CYP 1A1/1A2, 1A2, 2D6, and 2C9, inhibited CYP 3A4, and had no effect on 2B1/2B2. Conclusion. The findings would help mitigate toxicity and therapeutic failure especially in cases of coadministration of medications with food containing XA, with metabolism altered by the latter.http://dx.doi.org/10.1155/2022/4524877 |
| spellingShingle | Mary A. Agbenyeku Regina Appiah-Opong Ernest Obese Robert P. Biney Emmanuel A. Adakudugu Arnold D. Forkuo Silas A. Osei Mustapha K. Abeka Elvis O. Ameyaw In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes Advances in Pharmacological and Pharmaceutical Sciences |
| title | In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes |
| title_full | In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes |
| title_fullStr | In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes |
| title_full_unstemmed | In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes |
| title_short | In Vitro and In Vivo Effect of Xylopic Acid on Cytochrome P450 Enzymes |
| title_sort | in vitro and in vivo effect of xylopic acid on cytochrome p450 enzymes |
| url | http://dx.doi.org/10.1155/2022/4524877 |
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