Intracellular DNA Damage by Lysine-Acetylene Conjugates
Previously, we reported the design and properties of alkyne C-lysine conjugates, a powerful and tunable family of DNA cleaving reagents. We also reported that, upon photoactivation, these molecules are capable of inducing cancer cells death. To prove that the cell death stems from DNA cleavage by th...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Journal of Nucleic Acids |
| Online Access: | http://dx.doi.org/10.4061/2010/931394 |
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| author | Wang-Yong Yang Qiang Cao Catherine Callahan Catalina Galvis Qing-Xiang Sang Igor V. Alabugin |
| author_facet | Wang-Yong Yang Qiang Cao Catherine Callahan Catalina Galvis Qing-Xiang Sang Igor V. Alabugin |
| author_sort | Wang-Yong Yang |
| collection | DOAJ |
| description | Previously, we reported the design and properties of alkyne C-lysine conjugates, a powerful and tunable family of DNA cleaving reagents. We also reported that, upon photoactivation, these molecules are capable of inducing cancer cells death. To prove that the cell death stems from DNA cleavage by the conjugates, we investigated intracellular DNA damage induced by these molecules in LNCap cancer cells using single cell gel electrophoresis (SCGE) assays. The observation of highly efficient DNA damage confirmed that lysine acetylene conjugate is capable of cleaving the densely compacted intracellular DNA. This result provides a key mechanistic link between efficient DNA cleavage and cytotoxicity towards cancer cells for this family of light-activated anticancer agents. |
| format | Article |
| id | doaj-art-862d5481e7c54dc59204d0bef1182d2a |
| institution | OA Journals |
| issn | 2090-021X |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nucleic Acids |
| spelling | doaj-art-862d5481e7c54dc59204d0bef1182d2a2025-08-20T02:08:47ZengWileyJournal of Nucleic Acids2090-021X2010-01-01201010.4061/2010/931394931394Intracellular DNA Damage by Lysine-Acetylene ConjugatesWang-Yong Yang0Qiang Cao1Catherine Callahan2Catalina Galvis3Qing-Xiang Sang4Igor V. Alabugin5Department of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USADepartment of Chemistry and Biochemistry, Florida State University, Tallahassee, FL 32306-4390, USAPreviously, we reported the design and properties of alkyne C-lysine conjugates, a powerful and tunable family of DNA cleaving reagents. We also reported that, upon photoactivation, these molecules are capable of inducing cancer cells death. To prove that the cell death stems from DNA cleavage by the conjugates, we investigated intracellular DNA damage induced by these molecules in LNCap cancer cells using single cell gel electrophoresis (SCGE) assays. The observation of highly efficient DNA damage confirmed that lysine acetylene conjugate is capable of cleaving the densely compacted intracellular DNA. This result provides a key mechanistic link between efficient DNA cleavage and cytotoxicity towards cancer cells for this family of light-activated anticancer agents.http://dx.doi.org/10.4061/2010/931394 |
| spellingShingle | Wang-Yong Yang Qiang Cao Catherine Callahan Catalina Galvis Qing-Xiang Sang Igor V. Alabugin Intracellular DNA Damage by Lysine-Acetylene Conjugates Journal of Nucleic Acids |
| title | Intracellular DNA Damage by Lysine-Acetylene Conjugates |
| title_full | Intracellular DNA Damage by Lysine-Acetylene Conjugates |
| title_fullStr | Intracellular DNA Damage by Lysine-Acetylene Conjugates |
| title_full_unstemmed | Intracellular DNA Damage by Lysine-Acetylene Conjugates |
| title_short | Intracellular DNA Damage by Lysine-Acetylene Conjugates |
| title_sort | intracellular dna damage by lysine acetylene conjugates |
| url | http://dx.doi.org/10.4061/2010/931394 |
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