Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients
Crohn’s disease (CD) is an inflammatory disease of the gastrointestinal tract, with a rising incidence worldwide, particularly in children. CD is thought to arise due to an immune response to environmental factors. The role of bacteria in CD has recently been highlighted, and here, we examine the pr...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2018/9203908 |
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| _version_ | 1832550988223873024 |
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| author | Rachel V. Purcell Nadeem O. Kaakoush Hazel M. Mitchell John F. Pearson Jacqueline I. Keenan |
| author_facet | Rachel V. Purcell Nadeem O. Kaakoush Hazel M. Mitchell John F. Pearson Jacqueline I. Keenan |
| author_sort | Rachel V. Purcell |
| collection | DOAJ |
| description | Crohn’s disease (CD) is an inflammatory disease of the gastrointestinal tract, with a rising incidence worldwide, particularly in children. CD is thought to arise due to an immune response to environmental factors. The role of bacteria in CD has recently been highlighted, and here, we examine the prevalence of two bacterial species, enterotoxigenic Bacteroides fragilis (ETBF) and Fusobacterium nucleatum, implicated in gastrointestinal pathologies, in a pediatric CD cohort. Stool samples from 30 children with treatment-naïve CD and 30 age- and sex-matched controls were collected, and DNA was extracted. Quantitative PCR was used to determine the levels of ETBF and F. nucleatum in stool samples. Bacterial positivity and relative abundance were assessed between cases and controls and in relation to disease severity. No associations were found between colonization with ETBF and CD, or between colonization with either ETBF or F. nucleatum and disease severity or presence of C. concisus. However, a strong association was observed between positivity for F. nucleatum in the stool samples and the occurrence of CD in patients (25/30) as compared to controls (8/30) (P=0.003). F. nucleatum is more prevalent in the stool samples of pediatric CD patients, compared to healthy controls, and may have potential use as a biomarker of pediatric CD. |
| format | Article |
| id | doaj-art-8606f3ae8cc545f59ddac8e496a50f6d |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-8606f3ae8cc545f59ddac8e496a50f6d2025-02-03T06:05:15ZengWileyInternational Journal of Microbiology1687-918X1687-91982018-01-01201810.1155/2018/92039089203908Gastrointestinal Pathobionts in Pediatric Crohn’s Disease PatientsRachel V. Purcell0Nadeem O. Kaakoush1Hazel M. Mitchell2John F. Pearson3Jacqueline I. Keenan4Department of Surgery, University of Otago, Christchurch, New ZealandSchool of Medical Sciences, University of New South Wales, Sydney, NSW, AustraliaSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, AustraliaBiostatistics and Computational Biology Unit, University of Otago, Christchurch, New ZealandDepartment of Surgery, University of Otago, Christchurch, New ZealandCrohn’s disease (CD) is an inflammatory disease of the gastrointestinal tract, with a rising incidence worldwide, particularly in children. CD is thought to arise due to an immune response to environmental factors. The role of bacteria in CD has recently been highlighted, and here, we examine the prevalence of two bacterial species, enterotoxigenic Bacteroides fragilis (ETBF) and Fusobacterium nucleatum, implicated in gastrointestinal pathologies, in a pediatric CD cohort. Stool samples from 30 children with treatment-naïve CD and 30 age- and sex-matched controls were collected, and DNA was extracted. Quantitative PCR was used to determine the levels of ETBF and F. nucleatum in stool samples. Bacterial positivity and relative abundance were assessed between cases and controls and in relation to disease severity. No associations were found between colonization with ETBF and CD, or between colonization with either ETBF or F. nucleatum and disease severity or presence of C. concisus. However, a strong association was observed between positivity for F. nucleatum in the stool samples and the occurrence of CD in patients (25/30) as compared to controls (8/30) (P=0.003). F. nucleatum is more prevalent in the stool samples of pediatric CD patients, compared to healthy controls, and may have potential use as a biomarker of pediatric CD.http://dx.doi.org/10.1155/2018/9203908 |
| spellingShingle | Rachel V. Purcell Nadeem O. Kaakoush Hazel M. Mitchell John F. Pearson Jacqueline I. Keenan Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients International Journal of Microbiology |
| title | Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients |
| title_full | Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients |
| title_fullStr | Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients |
| title_full_unstemmed | Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients |
| title_short | Gastrointestinal Pathobionts in Pediatric Crohn’s Disease Patients |
| title_sort | gastrointestinal pathobionts in pediatric crohn s disease patients |
| url | http://dx.doi.org/10.1155/2018/9203908 |
| work_keys_str_mv | AT rachelvpurcell gastrointestinalpathobiontsinpediatriccrohnsdiseasepatients AT nadeemokaakoush gastrointestinalpathobiontsinpediatriccrohnsdiseasepatients AT hazelmmitchell gastrointestinalpathobiontsinpediatriccrohnsdiseasepatients AT johnfpearson gastrointestinalpathobiontsinpediatriccrohnsdiseasepatients AT jacquelineikeenan gastrointestinalpathobiontsinpediatriccrohnsdiseasepatients |