Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i>
<i>Fusarium</i> wilt in eggplant caused by <i>F. oxysporum</i> f. sp. <i>melongenae</i> is a major devastating soil-borne disease on a worldwide scale. Effectors play important roles in the interactions in pathogen–plant interactions. Identifying effectors is esse...
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2024-11-01
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| author | Jiayelu Wu Pengfei Wang Wuhong Wang Haijiao Hu Qingzhen Wei Chonglai Bao Yaqin Yan |
| author_facet | Jiayelu Wu Pengfei Wang Wuhong Wang Haijiao Hu Qingzhen Wei Chonglai Bao Yaqin Yan |
| author_sort | Jiayelu Wu |
| collection | DOAJ |
| description | <i>Fusarium</i> wilt in eggplant caused by <i>F. oxysporum</i> f. sp. <i>melongenae</i> is a major devastating soil-borne disease on a worldwide scale. Effectors play important roles in the interactions in pathogen–plant interactions. Identifying effectors is essential for elucidating the pathogenic mechanisms of phytopathogenic fungi. In this study, bioinformatic prediction approaches, including SignalP v5.0, TMHMM v2.0, WoLF PSORT, PredGPI, and EffectorP, were employed to screen for candidate secreted effector proteins (CSEPs) in <i>F. oxysporum</i> f. sp. <i>melongenae</i>. A total of 1019 proteins exhibiting characteristics typical of classical secretory proteins were identified, 301 of which demonstrated carbohydrate activity, and 194 CSEPs were identified. Furthermore, a total of 563 proteins from <i>F. oxysporum</i> f. sp. <i>melongenae</i> under induced conditions were identified using mass spectrometry-based label-free quantitative proteomics. These findings suggest a potential role of these CSEPs in the interaction between <i>F. oxysporum</i> f. sp. <i>melongenae</i> and eggplant, thereby contributing to a deeper understanding of the pathogenic mechanisms of <i>F. oxysporum</i> f. sp. <i>melongenae</i> and strategies for disease management. |
| format | Article |
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| institution | DOAJ |
| issn | 2309-608X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj-art-86051e82d1764b359b2c32cccff009ef2025-08-20T02:56:59ZengMDPI AGJournal of Fungi2309-608X2024-11-01101282810.3390/jof10120828Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i>Jiayelu Wu0Pengfei Wang1Wuhong Wang2Haijiao Hu3Qingzhen Wei4Chonglai Bao5Yaqin Yan6Institute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaZhejiang Normal University, Jinhua 321004, ChinaInstitute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaInstitute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaInstitute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaInstitute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, ChinaInstitute of Vegetable, Zhejiang Academy of Agricultural Science, Hangzhou 310021, China<i>Fusarium</i> wilt in eggplant caused by <i>F. oxysporum</i> f. sp. <i>melongenae</i> is a major devastating soil-borne disease on a worldwide scale. Effectors play important roles in the interactions in pathogen–plant interactions. Identifying effectors is essential for elucidating the pathogenic mechanisms of phytopathogenic fungi. In this study, bioinformatic prediction approaches, including SignalP v5.0, TMHMM v2.0, WoLF PSORT, PredGPI, and EffectorP, were employed to screen for candidate secreted effector proteins (CSEPs) in <i>F. oxysporum</i> f. sp. <i>melongenae</i>. A total of 1019 proteins exhibiting characteristics typical of classical secretory proteins were identified, 301 of which demonstrated carbohydrate activity, and 194 CSEPs were identified. Furthermore, a total of 563 proteins from <i>F. oxysporum</i> f. sp. <i>melongenae</i> under induced conditions were identified using mass spectrometry-based label-free quantitative proteomics. These findings suggest a potential role of these CSEPs in the interaction between <i>F. oxysporum</i> f. sp. <i>melongenae</i> and eggplant, thereby contributing to a deeper understanding of the pathogenic mechanisms of <i>F. oxysporum</i> f. sp. <i>melongenae</i> and strategies for disease management.https://www.mdpi.com/2309-608X/10/12/828effector proteins<i>F. oxysporum</i> f. sp. <i>melongenae</i>bioinformatic predictionLC-MS |
| spellingShingle | Jiayelu Wu Pengfei Wang Wuhong Wang Haijiao Hu Qingzhen Wei Chonglai Bao Yaqin Yan Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> Journal of Fungi effector proteins <i>F. oxysporum</i> f. sp. <i>melongenae</i> bioinformatic prediction LC-MS |
| title | Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> |
| title_full | Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> |
| title_fullStr | Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> |
| title_full_unstemmed | Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> |
| title_short | Comprehensive Genomic and Proteomic Analysis Identifies Effectors of <i>Fusarium oxysporum</i> f. sp. <i>melongenae</i> |
| title_sort | comprehensive genomic and proteomic analysis identifies effectors of i fusarium oxysporum i f sp i melongenae i |
| topic | effector proteins <i>F. oxysporum</i> f. sp. <i>melongenae</i> bioinformatic prediction LC-MS |
| url | https://www.mdpi.com/2309-608X/10/12/828 |
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