The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS
Camellia fascicularis, as a food and medicinal plant, is rich in polyphenols. Herein, digestion samples (DS) of C. fascicularis polyphenols (CFPs) were metabolically analyzed based on their bidirectional translocation in Caco-2 cells using ultraperformance liquid chromatography-electrospray ionizati...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000872 |
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| author | Shengjiang Duan Hao Zheng Junrong Tang Huan Kan Changwei Cao Zhijiao Shi Yun Liu |
| author_facet | Shengjiang Duan Hao Zheng Junrong Tang Huan Kan Changwei Cao Zhijiao Shi Yun Liu |
| author_sort | Shengjiang Duan |
| collection | DOAJ |
| description | Camellia fascicularis, as a food and medicinal plant, is rich in polyphenols. Herein, digestion samples (DS) of C. fascicularis polyphenols (CFPs) were metabolically analyzed based on their bidirectional translocation in Caco-2 cells using ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry. The results indicated that after DS transported via Caco-2 cell monolayer models nine polyphenol compounds in transit solution (TSB) from apical (AP) to basolateral (BL) side were upregulated, whereas seven in the transit solution (TSA) from BL to AP side were upregulated. In addition, 53 significantly different polyphenol compounds dominated by flavonoids were identified in the TSA vs. TSB groups, with a dominantly moderate degree of uptake. Among them, 40 polyphenol compounds were upregulated including eupatorin, 5,7,4′-trihydroxy-3,6,3′,5′-tetramethoxyflavone, and jaceosidin-7-O-glucoside, which might exhibit active transport, whereas the remaining 13 were downregulated and might exhibit passive diffusive transport. This study offers a rationale that further explored the bioactive mechanisms of CFPs. |
| format | Article |
| id | doaj-art-86045819b29042f9b63f7468e6dc8635 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-86045819b29042f9b63f7468e6dc86352025-02-12T05:32:42ZengElsevierFood Chemistry: X2590-15752025-01-0125102240The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MSShengjiang Duan0Hao Zheng1Junrong Tang2Huan Kan3Changwei Cao4Zhijiao Shi5Yun Liu6College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaForest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming 650224, China; Corresponding authors at: College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Corresponding authors at: College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China; Forest Resources Exploitation and Utilization Engineering Research Center for Grand Health of Yunnan Provincial Universities, Southwest Forestry University, Kunming 650224, China; Corresponding authors at: College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, China.Camellia fascicularis, as a food and medicinal plant, is rich in polyphenols. Herein, digestion samples (DS) of C. fascicularis polyphenols (CFPs) were metabolically analyzed based on their bidirectional translocation in Caco-2 cells using ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry. The results indicated that after DS transported via Caco-2 cell monolayer models nine polyphenol compounds in transit solution (TSB) from apical (AP) to basolateral (BL) side were upregulated, whereas seven in the transit solution (TSA) from BL to AP side were upregulated. In addition, 53 significantly different polyphenol compounds dominated by flavonoids were identified in the TSA vs. TSB groups, with a dominantly moderate degree of uptake. Among them, 40 polyphenol compounds were upregulated including eupatorin, 5,7,4′-trihydroxy-3,6,3′,5′-tetramethoxyflavone, and jaceosidin-7-O-glucoside, which might exhibit active transport, whereas the remaining 13 were downregulated and might exhibit passive diffusive transport. This study offers a rationale that further explored the bioactive mechanisms of CFPs.http://www.sciencedirect.com/science/article/pii/S2590157525000872Camellia fascicularisPolyphenolsCaco-2 cellsTransportMetabolites |
| spellingShingle | Shengjiang Duan Hao Zheng Junrong Tang Huan Kan Changwei Cao Zhijiao Shi Yun Liu The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS Food Chemistry: X Camellia fascicularis Polyphenols Caco-2 cells Transport Metabolites |
| title | The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS |
| title_full | The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS |
| title_fullStr | The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS |
| title_full_unstemmed | The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS |
| title_short | The transport of polyphenols from Camellia fascicularis in Caco-2 cells based on UPLC-ESI-MS/MS |
| title_sort | transport of polyphenols from camellia fascicularis in caco 2 cells based on uplc esi ms ms |
| topic | Camellia fascicularis Polyphenols Caco-2 cells Transport Metabolites |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000872 |
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