THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS
Aim. To determine the role of the endoplasmic reticulum (ER) in regulating gene expression in normal astrocytes and glioblastoma cells under the application of carbon nanoparticles as anti-tumor agents. Methods. Experiments were conducted on normal human astrocytes of the NHA/TS line and glioblas...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-04-01
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| Series: | Biotechnologia Acta |
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| Online Access: | https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Rudnytska2_2025%20.pdf |
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| author | O.V. Rudnytska |
| author_facet | O.V. Rudnytska |
| author_sort | O.V. Rudnytska |
| collection | DOAJ |
| description | Aim. To determine the role of the endoplasmic reticulum (ER) in regulating gene expression in normal astrocytes and glioblastoma cells under the application of carbon nanoparticles as anti-tumor agents.
Methods. Experiments were conducted on normal human astrocytes of the NHA/TS line and glioblastoma cell sublines U87MG. To study the impact of carbon nanoparticles, aqueous dispersions of graphene oxide (GO) and aqueous suspensions of single-walled carbon nanotubes (SWCNTs) were used. Methods of knockdown and silencing were applied to determine the role of the ERN1 signaling protein and its enzymatic activities. The measurement of mRNA and microRNA expression levels was carried out using RT-PCR.
Results. It was shown that the expression of serine biosynthesis genes depends on the enzymatic activities of the ERN1 signaling protein, which influences glioblastoma cell proliferation. Furthermore, the functional activity of ERN1 modifies the effects of hypoxia and nutrient deficiency on gene expression, which are significant factors in tumor growth. Comparison of the effects of SWCNTs and GO on normal astrocytes and glioblastoma cells demonstrated a more pronounced increase in studied gene expression levels in normal astrocytes than in glioblastoma cells.
Conclusions. Endoplasmic reticulum stress plays a vital role in regulating the expression of various regulatory genes in both normal and tumor cells. It also controls the effects of hypoxia and carbon nanoparticles on gene expression. |
| format | Article |
| id | doaj-art-85ff35125ba34fa5a98e52d42f0ed392 |
| institution | DOAJ |
| issn | 2410-7751 2410-776X |
| language | English |
| publishDate | 2025-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj-art-85ff35125ba34fa5a98e52d42f0ed3922025-08-20T03:16:31ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2025-04-01182101310.15407/biotech18.02.010THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLSO.V. Rudnytska0Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivAim. To determine the role of the endoplasmic reticulum (ER) in regulating gene expression in normal astrocytes and glioblastoma cells under the application of carbon nanoparticles as anti-tumor agents. Methods. Experiments were conducted on normal human astrocytes of the NHA/TS line and glioblastoma cell sublines U87MG. To study the impact of carbon nanoparticles, aqueous dispersions of graphene oxide (GO) and aqueous suspensions of single-walled carbon nanotubes (SWCNTs) were used. Methods of knockdown and silencing were applied to determine the role of the ERN1 signaling protein and its enzymatic activities. The measurement of mRNA and microRNA expression levels was carried out using RT-PCR. Results. It was shown that the expression of serine biosynthesis genes depends on the enzymatic activities of the ERN1 signaling protein, which influences glioblastoma cell proliferation. Furthermore, the functional activity of ERN1 modifies the effects of hypoxia and nutrient deficiency on gene expression, which are significant factors in tumor growth. Comparison of the effects of SWCNTs and GO on normal astrocytes and glioblastoma cells demonstrated a more pronounced increase in studied gene expression levels in normal astrocytes than in glioblastoma cells. Conclusions. Endoplasmic reticulum stress plays a vital role in regulating the expression of various regulatory genes in both normal and tumor cells. It also controls the effects of hypoxia and carbon nanoparticles on gene expression. https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Rudnytska2_2025%20.pdfer stressmrna and microrna expressionserine synthesis geneshypoxiacarbon nanoparticlesglioblastoma cellsnormal astrocytes |
| spellingShingle | O.V. Rudnytska THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS Biotechnologia Acta er stress mrna and microrna expression serine synthesis genes hypoxia carbon nanoparticles glioblastoma cells normal astrocytes |
| title | THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS |
| title_full | THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS |
| title_fullStr | THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS |
| title_full_unstemmed | THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS |
| title_short | THE MULTIFACETED ROLE OF ENDOPLASMIC RETICULUM STRESS IN THE REGULATION OF GENE EXPRESSION IN NORMAL ASTROCYTES AND GLIOBLASTOMA CELLS |
| title_sort | multifaceted role of endoplasmic reticulum stress in the regulation of gene expression in normal astrocytes and glioblastoma cells |
| topic | er stress mrna and microrna expression serine synthesis genes hypoxia carbon nanoparticles glioblastoma cells normal astrocytes |
| url | https://biotechnology.kiev.ua/images/BTA/2025/2_2025/Rudnytska2_2025%20.pdf |
| work_keys_str_mv | AT ovrudnytska themultifacetedroleofendoplasmicreticulumstressintheregulationofgeneexpressioninnormalastrocytesandglioblastomacells AT ovrudnytska multifacetedroleofendoplasmicreticulumstressintheregulationofgeneexpressioninnormalastrocytesandglioblastomacells |