Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch

Pea protein has broad market prospects due to its low cost, low fat content, absence of animal cholesterol and low allergenicity. However, its poor solubility limits its application. This paper employs the pH-shifting method to modify pea protein and explores the impact of the optimized modified pe...

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Main Authors: XU Zi-hui, XU Zi-han, LI Hao, JI Na, QIN Yang, XIONG Liu, SUN Qing-jie
Format: Article
Language:English
Published: Academy of National Food and Strategic Reserves Administration 2025-05-01
Series:Liang you shipin ke-ji
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Online Access:http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250316
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author XU Zi-hui
XU Zi-han
LI Hao
JI Na
QIN Yang
XIONG Liu
SUN Qing-jie
author_facet XU Zi-hui
XU Zi-han
LI Hao
JI Na
QIN Yang
XIONG Liu
SUN Qing-jie
author_sort XU Zi-hui
collection DOAJ
description Pea protein has broad market prospects due to its low cost, low fat content, absence of animal cholesterol and low allergenicity. However, its poor solubility limits its application. This paper employs the pH-shifting method to modify pea protein and explores the impact of the optimized modified pea protein on the functional properties of various starches. When the pH-shifted modified pea protein was incorporated into different starch systems, it was observed that the peak viscosity and breakdown value of potato starch decreased, whereas those of corn starch and pea starch, as well as their final viscosity and setback value, increased. Thermal analysis (DSC) revealed that the 7-day retrogradation rates of potato starch, corn starch, and pea starch decreased from 31.61%, 49.94%, and 31.85% to 10.75%, 21.70%, and 18.78%, respectively, indicating that the modified protein effectively inhibits the long-term retrogradation of starches. Resistance starch (RS) assays showed that the RS content of potato, corn, and pea starches increased significantly from 4.2%, 4.8%, and 6.3% to 21.4%, 20.7%, and 17.8%, respectively. These results demonstrate that pH-shifted modified pea protein holds significant potential in improving starch properties and enhancing starch resistance to digestion, particularly promising for broad applications in the food and functional materials industries.
format Article
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institution Kabale University
issn 1007-7561
language English
publishDate 2025-05-01
publisher Academy of National Food and Strategic Reserves Administration
record_format Article
series Liang you shipin ke-ji
spelling doaj-art-85f8253bc4c44866a65fb6232877f8b62025-08-20T03:46:21ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612025-05-0133315516210.16210/j.cnki.1007-7561.2025.03.015Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of StarchXU Zi-hui0XU Zi-han1 LI Hao2 JI Na3QIN Yang4XIONG Liu5SUN Qing-jie6College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, Shandong 257345, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, Shandong 257345, China College of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, Shandong 257345, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, ChinaCollege of Food Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong 266109, China;National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, Shandong 257345, China Pea protein has broad market prospects due to its low cost, low fat content, absence of animal cholesterol and low allergenicity. However, its poor solubility limits its application. This paper employs the pH-shifting method to modify pea protein and explores the impact of the optimized modified pea protein on the functional properties of various starches. When the pH-shifted modified pea protein was incorporated into different starch systems, it was observed that the peak viscosity and breakdown value of potato starch decreased, whereas those of corn starch and pea starch, as well as their final viscosity and setback value, increased. Thermal analysis (DSC) revealed that the 7-day retrogradation rates of potato starch, corn starch, and pea starch decreased from 31.61%, 49.94%, and 31.85% to 10.75%, 21.70%, and 18.78%, respectively, indicating that the modified protein effectively inhibits the long-term retrogradation of starches. Resistance starch (RS) assays showed that the RS content of potato, corn, and pea starches increased significantly from 4.2%, 4.8%, and 6.3% to 21.4%, 20.7%, and 17.8%, respectively. These results demonstrate that pH-shifted modified pea protein holds significant potential in improving starch properties and enhancing starch resistance to digestion, particularly promising for broad applications in the food and functional materials industries.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250316ph-shiftingmodified pea proteinproperties of starchlong-term retrogradationresistance to digestion
spellingShingle XU Zi-hui
XU Zi-han
LI Hao
JI Na
QIN Yang
XIONG Liu
SUN Qing-jie
Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
Liang you shipin ke-ji
ph-shifting
modified pea protein
properties of starch
long-term retrogradation
resistance to digestion
title Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
title_full Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
title_fullStr Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
title_full_unstemmed Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
title_short Modification of Pea Protein by pH-shifting Method and Its Impact on Functional Properties of Starch
title_sort modification of pea protein by ph shifting method and its impact on functional properties of starch
topic ph-shifting
modified pea protein
properties of starch
long-term retrogradation
resistance to digestion
url http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250316
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AT xuzihan modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch
AT lihao modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch
AT jina modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch
AT qinyang modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch
AT xiongliu modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch
AT sunqingjie modificationofpeaproteinbyphshiftingmethodanditsimpactonfunctionalpropertiesofstarch