LOCALIZATION OF REOVIRUS CELL ATTACHMENT PROTEIN ?l ON THE SURFACE OF THE REOVIRION USING IMMUNOFERRITIN ELECTRON MICROSCOPY
Purified reovirus type 3 (strain Dearing) was treated with monoclonal anti-?l antibody conjugated to ferritin and examined in the electron microscope. Virion associated ferritin molecules corresponding to locations of the ?l protein were observed. Electron microsocpy of thin sections of these prepa...
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| Language: | English |
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University of Tehran
1999-03-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
| Online Access: | https://jsciences.ut.ac.ir/article_31344_5fe8bfd268438f88f83cea09194abea8.pdf |
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| collection | DOAJ |
| description | Purified reovirus type 3 (strain Dearing) was treated with monoclonal anti-?l
antibody conjugated to ferritin and examined in the electron microscope. Virion associated
ferritin molecules corresponding to locations of the ?l protein were
observed. Electron microsocpy of thin sections of these preparations revealed that
ferritin conjugates were localized at the vertices of the viral icosahedron. Reovirus
cores possessing spikes and protein ?1, prepared by partial chymotrypsin digestion
of intact virions, were similarly treated and examined. Core-associated ferritin
molecules were found to be localized at positions corresponding to the tips of the
spikes. Such an association was not observed with the cores whose spikes had been
removed by alkali treatment. These results provide unequivocal evidence that protein
ol is present in icosahedrally-distributed locations, representing tips of reovirus core
spikes, on the surface of reovirus particles |
| format | Article |
| id | doaj-art-85f709fcb813466b955de7ceed230d3a |
| institution | DOAJ |
| issn | 1016-1104 2345-6914 |
| language | English |
| publishDate | 1999-03-01 |
| publisher | University of Tehran |
| record_format | Article |
| series | Journal of Sciences, Islamic Republic of Iran |
| spelling | doaj-art-85f709fcb813466b955de7ceed230d3a2025-08-20T03:08:46ZengUniversity of TehranJournal of Sciences, Islamic Republic of Iran1016-11042345-69141999-03-0110131344LOCALIZATION OF REOVIRUS CELL ATTACHMENT PROTEIN ?l ON THE SURFACE OF THE REOVIRION USING IMMUNOFERRITIN ELECTRON MICROSCOPYPurified reovirus type 3 (strain Dearing) was treated with monoclonal anti-?l antibody conjugated to ferritin and examined in the electron microscope. Virion associated ferritin molecules corresponding to locations of the ?l protein were observed. Electron microsocpy of thin sections of these preparations revealed that ferritin conjugates were localized at the vertices of the viral icosahedron. Reovirus cores possessing spikes and protein ?1, prepared by partial chymotrypsin digestion of intact virions, were similarly treated and examined. Core-associated ferritin molecules were found to be localized at positions corresponding to the tips of the spikes. Such an association was not observed with the cores whose spikes had been removed by alkali treatment. These results provide unequivocal evidence that protein ol is present in icosahedrally-distributed locations, representing tips of reovirus core spikes, on the surface of reovirus particleshttps://jsciences.ut.ac.ir/article_31344_5fe8bfd268438f88f83cea09194abea8.pdf |
| spellingShingle | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY Journal of Sciences, Islamic Republic of Iran |
| title | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY |
| title_full | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY |
| title_fullStr | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY |
| title_full_unstemmed | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY |
| title_short | LOCALIZATION OF REOVIRUS CELL
ATTACHMENT PROTEIN ?l ON THE SURFACE
OF THE REOVIRION USING IMMUNOFERRITIN
ELECTRON MICROSCOPY |
| title_sort | localization of reovirus cell attachment protein l on the surface of the reovirion using immunoferritin electron microscopy |
| url | https://jsciences.ut.ac.ir/article_31344_5fe8bfd268438f88f83cea09194abea8.pdf |