3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations

Abstract ABT-333 and ABT-072 are two potent non-nucleoside NS5B polymerase inhibitors designed for the treatment of the hepatitis C virus (HCV). These structural analogs differ only by a minor substituent change, which disrupts the planarity of the naphthyl group on the ABT-333 compound through the...

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Main Authors: Richard S. Hong, Alessandra Mattei, Mark E. Tuckerman, Ahmad Y. Sheikh
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Communications Chemistry
Online Access:https://doi.org/10.1038/s42004-025-01618-8
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author Richard S. Hong
Alessandra Mattei
Mark E. Tuckerman
Ahmad Y. Sheikh
author_facet Richard S. Hong
Alessandra Mattei
Mark E. Tuckerman
Ahmad Y. Sheikh
author_sort Richard S. Hong
collection DOAJ
description Abstract ABT-333 and ABT-072 are two potent non-nucleoside NS5B polymerase inhibitors designed for the treatment of the hepatitis C virus (HCV). These structural analogs differ only by a minor substituent change, which disrupts the planarity of the naphthyl group on the ABT-333 compound through the addition of a more flexible trans-olefin substituent. However, this minor change leads to significant differences in their conformational preferences and intermolecular interactions, resulting in a ripple effect with drug development implications, ranging from crystal polymorphism and low aqueous solubility to formulation development challenges. In this article, we demonstrate how a suite of molecular simulation approaches, including crystal structure prediction augmented with a new hydrate CSP algorithm, free-energy perturbation, molecular dynamics (MD) based solubility predictions, and topological assessment to evaluate surface re-crystallization tendencies, provide key atomistic-level insights into the differentiated performance of the two analogs. Through this study, we establish the importance of end-to-end physics-based modeling, which involves explicit considerations of 3-D structure and crystal packing interactions. This approach provides structural and energetic insights into the physicochemical properties and drug development challenges faced when designing best-in-class drug molecules.
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spelling doaj-art-85f6f2d1ba544dc1b4fe7ab8104834ee2025-08-20T03:42:34ZengNature PortfolioCommunications Chemistry2399-36692025-08-018111110.1038/s42004-025-01618-83D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulationsRichard S. Hong0Alessandra Mattei1Mark E. Tuckerman2Ahmad Y. Sheikh3AbbVie Inc., Molecular Profiling and Drug DeliveryAbbVie Inc., Molecular Profiling and Drug DeliveryDepartment of Chemistry, New York UniversityAbbVie Inc., Molecular Profiling and Drug DeliveryAbstract ABT-333 and ABT-072 are two potent non-nucleoside NS5B polymerase inhibitors designed for the treatment of the hepatitis C virus (HCV). These structural analogs differ only by a minor substituent change, which disrupts the planarity of the naphthyl group on the ABT-333 compound through the addition of a more flexible trans-olefin substituent. However, this minor change leads to significant differences in their conformational preferences and intermolecular interactions, resulting in a ripple effect with drug development implications, ranging from crystal polymorphism and low aqueous solubility to formulation development challenges. In this article, we demonstrate how a suite of molecular simulation approaches, including crystal structure prediction augmented with a new hydrate CSP algorithm, free-energy perturbation, molecular dynamics (MD) based solubility predictions, and topological assessment to evaluate surface re-crystallization tendencies, provide key atomistic-level insights into the differentiated performance of the two analogs. Through this study, we establish the importance of end-to-end physics-based modeling, which involves explicit considerations of 3-D structure and crystal packing interactions. This approach provides structural and energetic insights into the physicochemical properties and drug development challenges faced when designing best-in-class drug molecules.https://doi.org/10.1038/s42004-025-01618-8
spellingShingle Richard S. Hong
Alessandra Mattei
Mark E. Tuckerman
Ahmad Y. Sheikh
3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
Communications Chemistry
title 3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
title_full 3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
title_fullStr 3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
title_full_unstemmed 3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
title_short 3D conformation and crystal interaction insights into drug development challenges for HCV drug analogues via molecular simulations
title_sort 3d conformation and crystal interaction insights into drug development challenges for hcv drug analogues via molecular simulations
url https://doi.org/10.1038/s42004-025-01618-8
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