Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene
The increasing global demand for natural substances such as the sesquiterpene α-humulene makes optimizing microbial production essential. A production process using the versatile host <i>Cupriavidus necator</i> has been recently improved by adjusting the minimal media and process paramet...
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MDPI AG
2025-03-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/12/3/323 |
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| author | Lucas Becker Emely Dietz Dirk Holtmann |
| author_facet | Lucas Becker Emely Dietz Dirk Holtmann |
| author_sort | Lucas Becker |
| collection | DOAJ |
| description | The increasing global demand for natural substances such as the sesquiterpene α-humulene makes optimizing microbial production essential. A production process using the versatile host <i>Cupriavidus necator</i> has been recently improved by adjusting the minimal media and process parameters. Understanding microbial and process robustness is key to ensuring consistent performance under different conditions. This study is the first to investigate and quantify the robustness of microbial α-humulene production and biomass formation using <i>C. necator</i> pKR-hum. Established process improvements and the impact of common or individual precultures were analyzed and quantified for their effect on the robustness of product and biomass formation. We report a robust α-humulene production process with even more consistent biomass formation using <i>C. necator</i> pKR-hum. Even with a simulated process disturbance, 79% of the maximum α-humulene level was still produced. Overall, our results show that the α-humulene production process using <i>C. necator</i> pKR-hum is highly robust, demonstrating its resilience to process disturbances and suitability for further industrial applications. |
| format | Article |
| id | doaj-art-85eecacd37e94ec9b3f0fa7bf070d3f9 |
| institution | DOAJ |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj-art-85eecacd37e94ec9b3f0fa7bf070d3f92025-08-20T02:42:45ZengMDPI AGBioengineering2306-53542025-03-0112332310.3390/bioengineering12030323Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-HumuleneLucas Becker0Emely Dietz1Dirk Holtmann2Bioprocess Intensification, Institute of Bioprocess Engineering and Pharmaceutical Technology, Technische Hochschule Mittelhessen, 35390 Giessen, GermanyBioprocess Intensification, Institute of Bioprocess Engineering and Pharmaceutical Technology, Technische Hochschule Mittelhessen, 35390 Giessen, GermanyInstitute of Process Engineering in Life Sciences, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyThe increasing global demand for natural substances such as the sesquiterpene α-humulene makes optimizing microbial production essential. A production process using the versatile host <i>Cupriavidus necator</i> has been recently improved by adjusting the minimal media and process parameters. Understanding microbial and process robustness is key to ensuring consistent performance under different conditions. This study is the first to investigate and quantify the robustness of microbial α-humulene production and biomass formation using <i>C. necator</i> pKR-hum. Established process improvements and the impact of common or individual precultures were analyzed and quantified for their effect on the robustness of product and biomass formation. We report a robust α-humulene production process with even more consistent biomass formation using <i>C. necator</i> pKR-hum. Even with a simulated process disturbance, 79% of the maximum α-humulene level was still produced. Overall, our results show that the α-humulene production process using <i>C. necator</i> pKR-hum is highly robust, demonstrating its resilience to process disturbances and suitability for further industrial applications.https://www.mdpi.com/2306-5354/12/3/323process robustness<i>Cupriavidus necator</i>terpenesα-humulenepreculture variation |
| spellingShingle | Lucas Becker Emely Dietz Dirk Holtmann Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene Bioengineering process robustness <i>Cupriavidus necator</i> terpenes α-humulene preculture variation |
| title | Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene |
| title_full | Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene |
| title_fullStr | Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene |
| title_full_unstemmed | Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene |
| title_short | Robustness of the <i>Cupriavidus necator</i>-Catalyzed Production of α-Humulene |
| title_sort | robustness of the i cupriavidus necator i catalyzed production of α humulene |
| topic | process robustness <i>Cupriavidus necator</i> terpenes α-humulene preculture variation |
| url | https://www.mdpi.com/2306-5354/12/3/323 |
| work_keys_str_mv | AT lucasbecker robustnessoftheicupriavidusnecatoricatalyzedproductionofahumulene AT emelydietz robustnessoftheicupriavidusnecatoricatalyzedproductionofahumulene AT dirkholtmann robustnessoftheicupriavidusnecatoricatalyzedproductionofahumulene |