Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis
Secondary metabolites are crucial for plant defense. This study investigates the time-dependent transcriptomic and metabolomic responses of peanut hairy root cultures to a combined elicitor treatment of chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD). Differentially accumulated metabo...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X25000880 |
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| author | Apinun Limmongkon Phadtraphorn Chayjarung Chanyanut Pankaew Sompop Pinit Nitra Nuengchamnong Chonnikan Tothong |
| author_facet | Apinun Limmongkon Phadtraphorn Chayjarung Chanyanut Pankaew Sompop Pinit Nitra Nuengchamnong Chonnikan Tothong |
| author_sort | Apinun Limmongkon |
| collection | DOAJ |
| description | Secondary metabolites are crucial for plant defense. This study investigates the time-dependent transcriptomic and metabolomic responses of peanut hairy root cultures to a combined elicitor treatment of chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD). Differentially accumulated metabolites (DAMs), particularly stilbenes and phenolic/flavonoid compounds, increased significantly compared to the baseline group. Phenolic, flavonoid, and stilbene levels rose, while lignin content decreased over time. Transcriptomic analysis revealed upregulation of key genes in the phenylpropanoid pathway, including PAL, C4H, and 4CL, which correlated with elevated levels of hydroxybenzoic acid and related compounds. Genes involved in stilbene biosynthesis, such as STS, ROMT, R4DT-1, and R3’DT-4, as well as flavonoid biosynthesis genes, including CHS, CHI, F3H, CHR, FLS, and UGT72E, were also upregulated, corresponding to the accumulation of their respective metabolites. In contrast, lignin biosynthesis genes, such as HCT, CSE, CCoAOMT, CCR, CAD, and POD, were downregulated, while lignin-degrading genes were upregulated. This was further supported by tissue staining results and the reduction of lignin content during the elicitation period. The shift from lignin synthesis to degradation underscores a redirection of metabolic flux toward the production of defense-related secondary metabolites, particularly stilbenes, phenolics, and flavonoids. |
| format | Article |
| id | doaj-art-85d786e6a423452ca1ea387963a9a0c3 |
| institution | DOAJ |
| issn | 2667-064X |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj-art-85d786e6a423452ca1ea387963a9a0c32025-08-20T02:49:22ZengElsevierPlant Stress2667-064X2025-06-011610082310.1016/j.stress.2025.100823Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysisApinun Limmongkon0Phadtraphorn Chayjarung1Chanyanut Pankaew2Sompop Pinit3Nitra Nuengchamnong4Chonnikan Tothong5Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand; Centre of Excellence in Medical Biotechnology (CEMB), Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand; Corresponding author at: Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok, 65000, Thailand.Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandDepartment of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandScience Laboratory Centre, Faculty of Science, Naresuan University, Phitsanulok, 65000, ThailandDepartment of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, ThailandSecondary metabolites are crucial for plant defense. This study investigates the time-dependent transcriptomic and metabolomic responses of peanut hairy root cultures to a combined elicitor treatment of chitosan (CHT), methyl jasmonate (MeJA), and cyclodextrin (CD). Differentially accumulated metabolites (DAMs), particularly stilbenes and phenolic/flavonoid compounds, increased significantly compared to the baseline group. Phenolic, flavonoid, and stilbene levels rose, while lignin content decreased over time. Transcriptomic analysis revealed upregulation of key genes in the phenylpropanoid pathway, including PAL, C4H, and 4CL, which correlated with elevated levels of hydroxybenzoic acid and related compounds. Genes involved in stilbene biosynthesis, such as STS, ROMT, R4DT-1, and R3’DT-4, as well as flavonoid biosynthesis genes, including CHS, CHI, F3H, CHR, FLS, and UGT72E, were also upregulated, corresponding to the accumulation of their respective metabolites. In contrast, lignin biosynthesis genes, such as HCT, CSE, CCoAOMT, CCR, CAD, and POD, were downregulated, while lignin-degrading genes were upregulated. This was further supported by tissue staining results and the reduction of lignin content during the elicitation period. The shift from lignin synthesis to degradation underscores a redirection of metabolic flux toward the production of defense-related secondary metabolites, particularly stilbenes, phenolics, and flavonoids.http://www.sciencedirect.com/science/article/pii/S2667064X25000880TranscriptomeMetabolomePhenylpropanoidStilbeneLignin |
| spellingShingle | Apinun Limmongkon Phadtraphorn Chayjarung Chanyanut Pankaew Sompop Pinit Nitra Nuengchamnong Chonnikan Tothong Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis Plant Stress Transcriptome Metabolome Phenylpropanoid Stilbene Lignin |
| title | Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis |
| title_full | Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis |
| title_fullStr | Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis |
| title_full_unstemmed | Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis |
| title_short | Elicitor-induced stilbene production and lignin reduction in peanut hairy roots: Insights from transcriptomic and metabolomic analysis |
| title_sort | elicitor induced stilbene production and lignin reduction in peanut hairy roots insights from transcriptomic and metabolomic analysis |
| topic | Transcriptome Metabolome Phenylpropanoid Stilbene Lignin |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X25000880 |
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