Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments
Abstract The present research aimed to assess the metabolomic responses of wheat to olive mill wastewater (OMWW) and drought stress treatments. Wheat plants were cultivated under controlled conditions with the following treatments: control (75% field capacity, FC), OMWW (75 ml L−1), drought stress (...
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Nature Portfolio
2025-04-01
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| Online Access: | https://doi.org/10.1038/s41598-025-98547-2 |
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| author | Yousef Alhaj Hamoud Amal Mohamed AlGarawi Mohammad K. Okla Mohamed S. Sheteiwy Maha H. Khalaf Ibrahim A. Alaraidh Ali El-Keblawy Mohamed Abouleish Patricio Sandaña Elsayed Ahmed Elsadek Hiba Shaghaleh |
| author_facet | Yousef Alhaj Hamoud Amal Mohamed AlGarawi Mohammad K. Okla Mohamed S. Sheteiwy Maha H. Khalaf Ibrahim A. Alaraidh Ali El-Keblawy Mohamed Abouleish Patricio Sandaña Elsayed Ahmed Elsadek Hiba Shaghaleh |
| author_sort | Yousef Alhaj Hamoud |
| collection | DOAJ |
| description | Abstract The present research aimed to assess the metabolomic responses of wheat to olive mill wastewater (OMWW) and drought stress treatments. Wheat plants were cultivated under controlled conditions with the following treatments: control (75% field capacity, FC), OMWW (75 ml L−1), drought stress (40% FC, applied 30 days after sowing), and a combined treatment of OMWW and drought stress. Drought stress alone reduced grain yield by 67%, while the OMWW-treated plants resulted in a 29% reduction under stress relative to the control. OMWW application improved soil properties, enhancing organic matter and nutrient levels. Wheat grains from OMWW-treated plants exhibited higher sugar content and related enzyme activities, indicating improved metabolism, with significant increases in starch, fructose, and glucose levels alongside stable invertase and sucrose phosphate synthase activities. The study also noted substantial changes in amino acids, fatty acids, and phenolic acids in plants subjected to OMWW and drought stress. These modifications indicate OMWW’s capability to influence vital biochemical pathways and boost antioxidant capacities in wheat. In conclusion, OMWW proves to be an effective soil amendment that mitigates drought stress and contributes to the production of nutrient-rich, resilient wheat, underscoring its potential as a sustainable agricultural practice in water-scarce areas. |
| format | Article |
| id | doaj-art-85b3a732a5d443099b250748e46c551a |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-85b3a732a5d443099b250748e46c551a2025-08-20T03:13:54ZengNature PortfolioScientific Reports2045-23222025-04-0115111810.1038/s41598-025-98547-2Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatmentsYousef Alhaj Hamoud0Amal Mohamed AlGarawi1Mohammad K. Okla2Mohamed S. Sheteiwy3Maha H. Khalaf4Ibrahim A. Alaraidh5Ali El-Keblawy6Mohamed Abouleish7Patricio Sandaña8Elsayed Ahmed Elsadek9Hiba Shaghaleh10The National Key Laboratory of Water Disaster Prevention, Hohai UniversityBotany and Microbiology Department, College of Science, King Saud UniversityBotany and Microbiology Department, College of Science, King Saud UniversityDepartment of Integrative Agriculture, College of Agriculture and Veterinary Medicine, United Arab Emirates UniversityDepartment of Botany and Microbiology, Faculty of Science, Beni-Suef UniversityBotany and Microbiology Department, College of Science, King Saud UniversityDepartment of Applied Biology, College of Sciences, University of SharjahDepartment of Biology, Chemistry and Environmental Sciences, College of Arts and Sciences, American University of SharjahInstitute of Plant Production and Protection, Universidad Austral de ChileBiosystems Engineering Department, University of ArizonaThe Key Lab of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai UniversityAbstract The present research aimed to assess the metabolomic responses of wheat to olive mill wastewater (OMWW) and drought stress treatments. Wheat plants were cultivated under controlled conditions with the following treatments: control (75% field capacity, FC), OMWW (75 ml L−1), drought stress (40% FC, applied 30 days after sowing), and a combined treatment of OMWW and drought stress. Drought stress alone reduced grain yield by 67%, while the OMWW-treated plants resulted in a 29% reduction under stress relative to the control. OMWW application improved soil properties, enhancing organic matter and nutrient levels. Wheat grains from OMWW-treated plants exhibited higher sugar content and related enzyme activities, indicating improved metabolism, with significant increases in starch, fructose, and glucose levels alongside stable invertase and sucrose phosphate synthase activities. The study also noted substantial changes in amino acids, fatty acids, and phenolic acids in plants subjected to OMWW and drought stress. These modifications indicate OMWW’s capability to influence vital biochemical pathways and boost antioxidant capacities in wheat. In conclusion, OMWW proves to be an effective soil amendment that mitigates drought stress and contributes to the production of nutrient-rich, resilient wheat, underscoring its potential as a sustainable agricultural practice in water-scarce areas.https://doi.org/10.1038/s41598-025-98547-2AntioxidantDrought stressMetabolitesOlive mill wastewaterWheat |
| spellingShingle | Yousef Alhaj Hamoud Amal Mohamed AlGarawi Mohammad K. Okla Mohamed S. Sheteiwy Maha H. Khalaf Ibrahim A. Alaraidh Ali El-Keblawy Mohamed Abouleish Patricio Sandaña Elsayed Ahmed Elsadek Hiba Shaghaleh Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments Scientific Reports Antioxidant Drought stress Metabolites Olive mill wastewater Wheat |
| title | Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| title_full | Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| title_fullStr | Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| title_full_unstemmed | Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| title_short | Metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| title_sort | metabolomic responses of wheat grains to olive mill wastewater and drought stress treatments |
| topic | Antioxidant Drought stress Metabolites Olive mill wastewater Wheat |
| url | https://doi.org/10.1038/s41598-025-98547-2 |
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