Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions
This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-07-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/25/15/4629 |
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| author | Mona Ghazalová Pavlína Modlitbová Ota Samek Katarína Rebrošová Martin Šiler Jan Ježek Zdeněk Pilát |
| author_facet | Mona Ghazalová Pavlína Modlitbová Ota Samek Katarína Rebrošová Martin Šiler Jan Ježek Zdeněk Pilát |
| author_sort | Mona Ghazalová |
| collection | DOAJ |
| description | This pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor—demineralized water—inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument. |
| format | Article |
| id | doaj-art-859beaa48fac4b41b03071f42b3c18ac |
| institution | DOAJ |
| issn | 1424-8220 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj-art-859beaa48fac4b41b03071f42b3c18ac2025-08-20T03:04:43ZengMDPI AGSensors1424-82202025-07-012515462910.3390/s25154629Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress ConditionsMona Ghazalová0Pavlína Modlitbová1Ota Samek2Katarína Rebrošová3Martin Šiler4Jan Ježek5Zdeněk Pilát6Institute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicDepartment of Microbiology, Faculty of Medicine of Masaryk University and St. Anne’s, University Hospital, Pekařská 53, 656 91 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicInstitute of Scientific Instruments of the Czech Academy of Sciences, v.v.i., Královopolská 147, 612 00 Brno, Czech RepublicThis pilot study investigated the metabolic responses of five selected bacteria to physiological stress. Surface-enhanced Raman spectroscopy was used to analyze spectral changes associated with the release of adenine, a key metabolite indicative of stress conditions. Laboratory-synthesized spherical silver and gold nanoparticles, which remained stable over an extended period, were employed as enhanced surfaces. Bacterial cultures were analyzed under standard conditions and in the presence of a selected stressor—demineralized water—inducing osmotic stress. The results showed that the adenine signal originated from metabolites released into the surrounding environment rather than directly from the bacterial cell wall. The study confirms the suitability of these cost-effective and easily synthesized stable nanoparticles for the qualitative detection of bacterial metabolites using a commercially available Raman instrument.https://www.mdpi.com/1424-8220/25/15/4629SERSAg-NPsAu-NPsphenylalanine<i>Escherichia coli</i><i>Staphylococcus aureus</i> |
| spellingShingle | Mona Ghazalová Pavlína Modlitbová Ota Samek Katarína Rebrošová Martin Šiler Jan Ježek Zdeněk Pilát Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions Sensors SERS Ag-NPs Au-NPs phenylalanine <i>Escherichia coli</i> <i>Staphylococcus aureus</i> |
| title | Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions |
| title_full | Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions |
| title_fullStr | Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions |
| title_full_unstemmed | Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions |
| title_short | Surface-Enhanced Raman Spectroscopy for Adenine Detection in Five Selected Bacterial Strains Under Stress Conditions |
| title_sort | surface enhanced raman spectroscopy for adenine detection in five selected bacterial strains under stress conditions |
| topic | SERS Ag-NPs Au-NPs phenylalanine <i>Escherichia coli</i> <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/1424-8220/25/15/4629 |
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