OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience
Background: Performance of OMNIgene.SPUTUM (OM-S) for transporting sputum was evaluated. Methods: This exploratory study was conducted during January-December 2019 at four near and one distant healthcare-facilities of Dhaka. Smear-positive pulmonary TB patients’ sputa were collected, divided into ‘O...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-08-01
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| Series: | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405579425000439 |
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| author | Senjuti Kabir Sabrina Choudhury Mohammad Khaja Mafij Uddin M.D. Fahim Ather Syed Mohammad Mazidur Rahman Pronab Kumar Modak Mohammad Samsuddoha Sarker Shanchay Tanjina Rahman Shahriar Ahmed Sayera Banu |
| author_facet | Senjuti Kabir Sabrina Choudhury Mohammad Khaja Mafij Uddin M.D. Fahim Ather Syed Mohammad Mazidur Rahman Pronab Kumar Modak Mohammad Samsuddoha Sarker Shanchay Tanjina Rahman Shahriar Ahmed Sayera Banu |
| author_sort | Senjuti Kabir |
| collection | DOAJ |
| description | Background: Performance of OMNIgene.SPUTUM (OM-S) for transporting sputum was evaluated. Methods: This exploratory study was conducted during January-December 2019 at four near and one distant healthcare-facilities of Dhaka. Smear-positive pulmonary TB patients’ sputa were collected, divided into ‘OM-S untreated’ and ‘OM-S treated’ portions, and transported to testing laboratory, Dhaka, on same-day from near-sites, and through courier from distant-site for smear-microscopy, culture, and Xpert MTB/RIF (Xpert) testing. Subset of ‘OM-S treated’ sample was tested with Xpert without centrifugation. Test results of all portions were compared in between. Results: Total 444 participants were enrolled (near-sites:198, distant-site: 246). All test results were comparable in both portions for near-sites. For distant-site, smear-microscopy’s positivity was reduced by 4.1 % in ‘OM-S treated’, Xpert showed 100 % concordance in both portions, and culture was higher in ‘OM-S treated’ than ‘OM-S untreated’ (92.3 % vs 89.4 %; p = 0.288). Primary contamination rate in ‘OM-S treated’ was lower than ‘OM-S untreated’ (2.0 % vs 9.8 %; p < 0.05). For all sites, median (IQR) time-to-culture positivity was 35 (28, 42) days in both portions. Xpert positivity was 99 % concordant in ‘OM-S treated’ regardless of centrifugation. Conclusions: OM-S is safe for sputum transportation. OM-S mixed sputum can be tested with Xpert and culture. Further studies can validate findings and assess cost-effectiveness. |
| format | Article |
| id | doaj-art-85986b4c42454a59b72050b4d2569565 |
| institution | Kabale University |
| issn | 2405-5794 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Clinical Tuberculosis and Other Mycobacterial Diseases |
| spelling | doaj-art-85986b4c42454a59b72050b4d25695652025-08-20T03:58:00ZengElsevierJournal of Clinical Tuberculosis and Other Mycobacterial Diseases2405-57942025-08-014010055210.1016/j.jctube.2025.100552OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experienceSenjuti Kabir0Sabrina Choudhury1Mohammad Khaja Mafij Uddin2M.D. Fahim Ather3Syed Mohammad Mazidur Rahman4Pronab Kumar Modak5Mohammad Samsuddoha Sarker Shanchay6Tanjina Rahman7Shahriar Ahmed8Sayera Banu9Infectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, Bangladesh; Graduate Research Assistant, University of Virginia, Charlottesville, VA, USAInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshNational Tuberculosis Control Programme, Director General of Health Services, Mohakhali, Dhaka, Bangladesh300 bedded Hospital, Narayanganj, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, BangladeshInfectious Diseases Division, icddr,b, Mohakhali, Dhaka, Bangladesh; Corresponding author at: Sayera Banu, Head, Programme for Emerging and Re-emerging Infections, Infectious Diseases Division, 68, Shaheed Tajuddin Ahmed Sarani, icddr,b, Mohakhali, Dhaka, Banlgadesh.Background: Performance of OMNIgene.SPUTUM (OM-S) for transporting sputum was evaluated. Methods: This exploratory study was conducted during January-December 2019 at four near and one distant healthcare-facilities of Dhaka. Smear-positive pulmonary TB patients’ sputa were collected, divided into ‘OM-S untreated’ and ‘OM-S treated’ portions, and transported to testing laboratory, Dhaka, on same-day from near-sites, and through courier from distant-site for smear-microscopy, culture, and Xpert MTB/RIF (Xpert) testing. Subset of ‘OM-S treated’ sample was tested with Xpert without centrifugation. Test results of all portions were compared in between. Results: Total 444 participants were enrolled (near-sites:198, distant-site: 246). All test results were comparable in both portions for near-sites. For distant-site, smear-microscopy’s positivity was reduced by 4.1 % in ‘OM-S treated’, Xpert showed 100 % concordance in both portions, and culture was higher in ‘OM-S treated’ than ‘OM-S untreated’ (92.3 % vs 89.4 %; p = 0.288). Primary contamination rate in ‘OM-S treated’ was lower than ‘OM-S untreated’ (2.0 % vs 9.8 %; p < 0.05). For all sites, median (IQR) time-to-culture positivity was 35 (28, 42) days in both portions. Xpert positivity was 99 % concordant in ‘OM-S treated’ regardless of centrifugation. Conclusions: OM-S is safe for sputum transportation. OM-S mixed sputum can be tested with Xpert and culture. Further studies can validate findings and assess cost-effectiveness.http://www.sciencedirect.com/science/article/pii/S2405579425000439OMNIgene.SPUTUMSputum transportationRoom temperatureTuberculosis |
| spellingShingle | Senjuti Kabir Sabrina Choudhury Mohammad Khaja Mafij Uddin M.D. Fahim Ather Syed Mohammad Mazidur Rahman Pronab Kumar Modak Mohammad Samsuddoha Sarker Shanchay Tanjina Rahman Shahriar Ahmed Sayera Banu OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience Journal of Clinical Tuberculosis and Other Mycobacterial Diseases OMNIgene.SPUTUM Sputum transportation Room temperature Tuberculosis |
| title | OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience |
| title_full | OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience |
| title_fullStr | OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience |
| title_full_unstemmed | OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience |
| title_short | OMNIgene.SPUTUM for transporting sputum in ambient temperature for tuberculosis testing: Bangladesh experience |
| title_sort | omnigene sputum for transporting sputum in ambient temperature for tuberculosis testing bangladesh experience |
| topic | OMNIgene.SPUTUM Sputum transportation Room temperature Tuberculosis |
| url | http://www.sciencedirect.com/science/article/pii/S2405579425000439 |
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