Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model

Phellodendri cortex (PC), the dried trunk bark of <i>Phellodendron amurense</i> RUPR, has traditionally been used to treat patients who suffer from gastroenteritis, abdominal pain or diarrhea. Its major bioactive compounds include alkaloids and limonin, and many physiological activities...

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Main Authors: Seong-Kyeom Kim, Ji-Won Do, Seong-Kyun Lee, Jae-Ho Park, Ju-Hyoung Kim, Heung-Bin Lim
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/8/1795
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author Seong-Kyeom Kim
Ji-Won Do
Seong-Kyun Lee
Jae-Ho Park
Ju-Hyoung Kim
Heung-Bin Lim
author_facet Seong-Kyeom Kim
Ji-Won Do
Seong-Kyun Lee
Jae-Ho Park
Ju-Hyoung Kim
Heung-Bin Lim
author_sort Seong-Kyeom Kim
collection DOAJ
description Phellodendri cortex (PC), the dried trunk bark of <i>Phellodendron amurense</i> RUPR, has traditionally been used to treat patients who suffer from gastroenteritis, abdominal pain or diarrhea. Its major bioactive compounds include alkaloids and limonin, and many physiological activities including anti-microbial, anti-ulcer and anti-cancer as well as anti-inflammation have been reported. Although PC is an effective anti-inflammatory natural substance that inhibits the inflammatory response, its effect on allergic asthma has not yet been investigated. The aim of this study was to evaluate the anti-asthmatic effects of PC in an ovalbumin (OVA)-induced murine model of asthma. As a result, PC inhibited airway eosinophil accumulation, the influx of inflammatory cells, airway hyperresponsiveness (AHR), production of Th2 cytokines (IL-4, IL-5 and IL-13) and tumor necrosis factor-α (TNF-α) in the bronchoalveolar lavage fluid and/or lung, as well as OVA-specific immunoglobulin E (IgE) in the serum. Furthermore, PC suppressed the gene expression of IL-4, IL-5, IL-13, TARC and CCR3, and attenuated unique histological changes that are associated with airway inflammatory reactions including the infiltration of various inflammatory cells, collagen deposition and goblet cell hyperplasia in lung tissues. These results indicate that PC may have preventive and/or therapeutic effects for allergic asthma via the inhibition of cytokines, chemokines and chemokine receptors associated with allergic inflammation.
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spelling doaj-art-859837dfd64b4ff8a447f233994c622f2025-08-20T02:18:04ZengMDPI AGMolecules1420-30492025-04-01308179510.3390/molecules30081795Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma ModelSeong-Kyeom Kim0Ji-Won Do1Seong-Kyun Lee2Jae-Ho Park3Ju-Hyoung Kim4Heung-Bin Lim5Bureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaBureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaBureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaBureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaBureau of Research & Development, Chungcheongbuk-do Agricultural Research and Extension Services, Cheongju 28130, Republic of KoreaDepartment of Industrial Plant Science & Technology, Chungbuk National University, Cheongju 28644, Republic of KoreaPhellodendri cortex (PC), the dried trunk bark of <i>Phellodendron amurense</i> RUPR, has traditionally been used to treat patients who suffer from gastroenteritis, abdominal pain or diarrhea. Its major bioactive compounds include alkaloids and limonin, and many physiological activities including anti-microbial, anti-ulcer and anti-cancer as well as anti-inflammation have been reported. Although PC is an effective anti-inflammatory natural substance that inhibits the inflammatory response, its effect on allergic asthma has not yet been investigated. The aim of this study was to evaluate the anti-asthmatic effects of PC in an ovalbumin (OVA)-induced murine model of asthma. As a result, PC inhibited airway eosinophil accumulation, the influx of inflammatory cells, airway hyperresponsiveness (AHR), production of Th2 cytokines (IL-4, IL-5 and IL-13) and tumor necrosis factor-α (TNF-α) in the bronchoalveolar lavage fluid and/or lung, as well as OVA-specific immunoglobulin E (IgE) in the serum. Furthermore, PC suppressed the gene expression of IL-4, IL-5, IL-13, TARC and CCR3, and attenuated unique histological changes that are associated with airway inflammatory reactions including the infiltration of various inflammatory cells, collagen deposition and goblet cell hyperplasia in lung tissues. These results indicate that PC may have preventive and/or therapeutic effects for allergic asthma via the inhibition of cytokines, chemokines and chemokine receptors associated with allergic inflammation.https://www.mdpi.com/1420-3049/30/8/1795phellodendri cortexalkaloidasthmaairway inflammationairway hypersensitivity
spellingShingle Seong-Kyeom Kim
Ji-Won Do
Seong-Kyun Lee
Jae-Ho Park
Ju-Hyoung Kim
Heung-Bin Lim
Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
Molecules
phellodendri cortex
alkaloid
asthma
airway inflammation
airway hypersensitivity
title Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
title_full Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
title_fullStr Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
title_full_unstemmed Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
title_short Inhibitory Effects of Phellodendri Cortex Against Airway Inflammation and Hyperresponsiveness in Ovalbumin-Induced Murine Asthma Model
title_sort inhibitory effects of phellodendri cortex against airway inflammation and hyperresponsiveness in ovalbumin induced murine asthma model
topic phellodendri cortex
alkaloid
asthma
airway inflammation
airway hypersensitivity
url https://www.mdpi.com/1420-3049/30/8/1795
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