Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis
Circular RNA Ribonuclease P RNA Component H1 (circ_RPPH1) and microRNA (miRNA) miR-1296-5p play a crucial role in breast cancer (BC), but the molecular mechanism is vague. Evidence showed that miR-1296-5p can activate tripartite motif-containing 14 (TRIM14). Clinical indications of eighty BC patient...
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| Format: | Article |
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Taylor & Francis Group
2024-12-01
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| Series: | Cancer Biology & Therapy |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15384047.2024.2360768 |
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| author | Jing Jiang Shenghong Shi Wei Zhang Chao Li Long Sun Qidong Ge Xujun Li |
| author_facet | Jing Jiang Shenghong Shi Wei Zhang Chao Li Long Sun Qidong Ge Xujun Li |
| author_sort | Jing Jiang |
| collection | DOAJ |
| description | Circular RNA Ribonuclease P RNA Component H1 (circ_RPPH1) and microRNA (miRNA) miR-1296-5p play a crucial role in breast cancer (BC), but the molecular mechanism is vague. Evidence showed that miR-1296-5p can activate tripartite motif-containing 14 (TRIM14). Clinical indications of eighty BC patients were collected and the circ_RPPH1 expression was detected using real-time quantitative PCR. MCF-7 and MDA-MB-231 cells were transfected with overexpression or knockdown of circ_RPPH1, miR-1296-5p, or TRIM14. Cell counting kit-8, cell cloning formation, wound healing, Transwell, and flow cytometry assays were performed to investigate the malignant phenotype of BC. The dual-luciferase reporter gene analyses were applied to reveal the interaction between these target genes. Subcutaneous tumorigenic model mice were established with circ_RPPH1 overexpression MDA-MB-231 cells in vivo; the tumor weight and volume, levels of miR-1296-5 and TRIM14 mRNA were measured. Western blot and immunohistochemistry were used to detect TRIM14 in cells and mice. Circ_RPPH1 levels were notably higher in BC patients and have been found to promote cell proliferation, invasion, and migration of BC cells. Circ_RPPH1 altered cell cycle and hindered apoptosis. Circ_RPPH1 knockdown or miR-1296-5p overexpression inhibited the malignant phenotype of BC. Furthermore, miR-1296-5p knockdown reversed circ_RPPH1’s promotion effects on BC. Interestingly, TRIM14 overexpression counteracts the inhibitory effects of miR-1296-5p overexpression and circ_RPPH1 silencing on BC. Moreover, in BC tumor-bearing mice, circ_RPPH1 overexpression led to increased TRIM14 expression and facilitated tumor growth. Circ_RPPH1 enhanced BC progression through miR-1296-5p/TRIM14 axis, indicating its potential as a biomarker and therapeutic target in BC. |
| format | Article |
| id | doaj-art-8591c59c8be8464ea375a865f2b883a0 |
| institution | DOAJ |
| issn | 1538-4047 1555-8576 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Cancer Biology & Therapy |
| spelling | doaj-art-8591c59c8be8464ea375a865f2b883a02025-08-20T02:49:46ZengTaylor & Francis GroupCancer Biology & Therapy1538-40471555-85762024-12-0125110.1080/15384047.2024.2360768Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axisJing Jiang0Shenghong Shi1Wei Zhang2Chao Li3Long Sun4Qidong Ge5Xujun Li6Department of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaDepartment of Breast Surgery, Ningbo, Zhejiang, ChinaCircular RNA Ribonuclease P RNA Component H1 (circ_RPPH1) and microRNA (miRNA) miR-1296-5p play a crucial role in breast cancer (BC), but the molecular mechanism is vague. Evidence showed that miR-1296-5p can activate tripartite motif-containing 14 (TRIM14). Clinical indications of eighty BC patients were collected and the circ_RPPH1 expression was detected using real-time quantitative PCR. MCF-7 and MDA-MB-231 cells were transfected with overexpression or knockdown of circ_RPPH1, miR-1296-5p, or TRIM14. Cell counting kit-8, cell cloning formation, wound healing, Transwell, and flow cytometry assays were performed to investigate the malignant phenotype of BC. The dual-luciferase reporter gene analyses were applied to reveal the interaction between these target genes. Subcutaneous tumorigenic model mice were established with circ_RPPH1 overexpression MDA-MB-231 cells in vivo; the tumor weight and volume, levels of miR-1296-5 and TRIM14 mRNA were measured. Western blot and immunohistochemistry were used to detect TRIM14 in cells and mice. Circ_RPPH1 levels were notably higher in BC patients and have been found to promote cell proliferation, invasion, and migration of BC cells. Circ_RPPH1 altered cell cycle and hindered apoptosis. Circ_RPPH1 knockdown or miR-1296-5p overexpression inhibited the malignant phenotype of BC. Furthermore, miR-1296-5p knockdown reversed circ_RPPH1’s promotion effects on BC. Interestingly, TRIM14 overexpression counteracts the inhibitory effects of miR-1296-5p overexpression and circ_RPPH1 silencing on BC. Moreover, in BC tumor-bearing mice, circ_RPPH1 overexpression led to increased TRIM14 expression and facilitated tumor growth. Circ_RPPH1 enhanced BC progression through miR-1296-5p/TRIM14 axis, indicating its potential as a biomarker and therapeutic target in BC.https://www.tandfonline.com/doi/10.1080/15384047.2024.2360768Breast cancercircular RNA ribonuclease P RNA component H1microRNA-1296-5ptripartite motif-containing 14 |
| spellingShingle | Jing Jiang Shenghong Shi Wei Zhang Chao Li Long Sun Qidong Ge Xujun Li Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis Cancer Biology & Therapy Breast cancer circular RNA ribonuclease P RNA component H1 microRNA-1296-5p tripartite motif-containing 14 |
| title | Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis |
| title_full | Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis |
| title_fullStr | Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis |
| title_full_unstemmed | Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis |
| title_short | Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis |
| title_sort | circ rpph1 facilitates progression of breast cancer via mir 1296 5p trim14 axis |
| topic | Breast cancer circular RNA ribonuclease P RNA component H1 microRNA-1296-5p tripartite motif-containing 14 |
| url | https://www.tandfonline.com/doi/10.1080/15384047.2024.2360768 |
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