DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1

Dengue virus (DENV) is one of the major arboviruses that pose a serious threat to global human health. However, there is currently no specific antiviral drug available for the treatment of DENV infection. DDX17, a member of the DExD/H-box helicase family, has been implicated in the replication proce...

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Main Authors: Peijun Han, Wei Ye, Hongwei Ma, Yangchao Dong, Xin Lv, He Liu, Linfeng Cheng, Liang Zhang, Sumin Li, Yingfeng Lei, Fanglin Zhang
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:International Journal of Medical Microbiology
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Online Access:http://www.sciencedirect.com/science/article/pii/S1438422125000104
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author Peijun Han
Wei Ye
Hongwei Ma
Yangchao Dong
Xin Lv
He Liu
Linfeng Cheng
Liang Zhang
Sumin Li
Yingfeng Lei
Fanglin Zhang
author_facet Peijun Han
Wei Ye
Hongwei Ma
Yangchao Dong
Xin Lv
He Liu
Linfeng Cheng
Liang Zhang
Sumin Li
Yingfeng Lei
Fanglin Zhang
author_sort Peijun Han
collection DOAJ
description Dengue virus (DENV) is one of the major arboviruses that pose a serious threat to global human health. However, there is currently no specific antiviral drug available for the treatment of DENV infection. DDX17, a member of the DExD/H-box helicase family, has been implicated in the replication processes of various viruses. Our research group discovered that during the early stages of dengue virus replication, DDX17 promotes viral replication and suppresses the activity of the IFN promoter. Furthermore, DDX17 binds to viral dsRNA and interacts with G3BP1, a component of stress granules (SGs), to inhibit SG formation, thereby enhancing viral replication. By elucidating the role of DDX17 in the early stages of dengue virus replication, our findings provide valuable insights into host-pathogen interactions during DENV infection, offering potential therapeutic perspectives.
format Article
id doaj-art-8590cfabaa754ce889f0e1864c8d38cc
institution Kabale University
issn 1438-4221
language English
publishDate 2025-06-01
publisher Elsevier
record_format Article
series International Journal of Medical Microbiology
spelling doaj-art-8590cfabaa754ce889f0e1864c8d38cc2025-08-20T03:45:30ZengElsevierInternational Journal of Medical Microbiology1438-42212025-06-0131915165410.1016/j.ijmm.2025.151654DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1Peijun Han0Wei Ye1Hongwei Ma2Yangchao Dong3Xin Lv4He Liu5Linfeng Cheng6Liang Zhang7Sumin Li8Yingfeng Lei9Fanglin Zhang10Department of Aerospace Hygiene, School of Aerospace Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Aerospace Hygiene, School of Aerospace Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, ChinaDepartment of Microbiology & Pathogen Biology, School of Basic Medicine, Air Force Medical University (the Fourth Military Medical University), Xi'an, Shaanxi 710032, China; Corresponding author.Dengue virus (DENV) is one of the major arboviruses that pose a serious threat to global human health. However, there is currently no specific antiviral drug available for the treatment of DENV infection. DDX17, a member of the DExD/H-box helicase family, has been implicated in the replication processes of various viruses. Our research group discovered that during the early stages of dengue virus replication, DDX17 promotes viral replication and suppresses the activity of the IFN promoter. Furthermore, DDX17 binds to viral dsRNA and interacts with G3BP1, a component of stress granules (SGs), to inhibit SG formation, thereby enhancing viral replication. By elucidating the role of DDX17 in the early stages of dengue virus replication, our findings provide valuable insights into host-pathogen interactions during DENV infection, offering potential therapeutic perspectives.http://www.sciencedirect.com/science/article/pii/S1438422125000104Dengue virusDDX17IFN-βG3BP1DExD/H-boxSG
spellingShingle Peijun Han
Wei Ye
Hongwei Ma
Yangchao Dong
Xin Lv
He Liu
Linfeng Cheng
Liang Zhang
Sumin Li
Yingfeng Lei
Fanglin Zhang
DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
International Journal of Medical Microbiology
Dengue virus
DDX17
IFN-β
G3BP1
DExD/H-box
SG
title DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
title_full DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
title_fullStr DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
title_full_unstemmed DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
title_short DDX17 promotes DENV-2 replication via interaction with viral dsRNA and G3BP1
title_sort ddx17 promotes denv 2 replication via interaction with viral dsrna and g3bp1
topic Dengue virus
DDX17
IFN-β
G3BP1
DExD/H-box
SG
url http://www.sciencedirect.com/science/article/pii/S1438422125000104
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