Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells
Objective: The objective of this study was to investigate the protective effects and the underlying mechanisms of resveratrol against hydrogen peroxide (H2O2 )-induced oxidative stress in healthy human and lung cancer cells. Materials and Methods: The cytotoxic doses and IC50 values of resveratrol a...
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Istanbul University Press
2024-05-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C49F458445A041BA8E77C8287F678B92 |
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| author | Öznur Yurdakul Aysun Özkan |
| author_facet | Öznur Yurdakul Aysun Özkan |
| author_sort | Öznur Yurdakul |
| collection | DOAJ |
| description | Objective: The objective of this study was to investigate the protective effects and the underlying mechanisms of resveratrol against hydrogen peroxide (H2O2 )-induced oxidative stress in healthy human and lung cancer cells. Materials and Methods: The cytotoxic doses and IC50 values of resveratrol and hydrogen peroxide for cells were determined by the Cell Titer Blue-Viability Assay kit. The amount of malondialdehyde (MDA) was determined by fluorescence spectrophotometer. The amount of intracellular reduced glutathione level and antioxidant enzyme activities were analyzed by spectrophotometric methods. Results: In both cells, H2O2 treatment alone (IC50 and IC50) increased MDA, glutathione reductase, glutathione S-transferase, selenium-dependent glutathione peroxidase and non-selenium-dependent glutathione peroxidase activities, but glutathione levels decreased compared to the H2O + 2 resveratrol treatment. In addition, high doses of resveratrol alone (IC50 and IC70) induced more oxidative stress in cancer cells than in healthy cells. High doses of resveratrol alone (IC50 and IC70) also showed cytotoxic effects in cells and decreased cell viability. Resveratrol caused more cytotoxic effects in cancer cells compared to healthy cells. Conclusion: The results of this study show that the increase in MDA level and antioxidant enzyme activity caused by highdose resveratrol treatment reveals the prooxidant effect of resveratrol. Our results also showed an antioxidant effect by reducing oxidative stress in cells pre-incubated with low-dose resveratrol and then exposed to H2O2 . Resveratrol has a dose-dependent biphasic (pro/antioxidant) effect on the antioxidant mechanism of cells. However, more research is needed to confirm this |
| format | Article |
| id | doaj-art-8585eec281f841de9b5f960bb5c0f3d1 |
| institution | Kabale University |
| issn | 2618-6144 |
| language | English |
| publishDate | 2024-05-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-8585eec281f841de9b5f960bb5c0f3d12025-08-20T03:48:45ZengIstanbul University PressEuropean Journal of Biology2618-61442024-05-01831424910.26650/EurJBiol.2024.1395956123456Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer CellsÖznur Yurdakul0https://orcid.org/0000-0002-4302-0816Aysun Özkan1https://orcid.org/0000-0002-9403-3342Akdeniz Üniversitesi, Antalya, TurkiyeAkdeniz Üniversitesi, Antalya, TurkiyeObjective: The objective of this study was to investigate the protective effects and the underlying mechanisms of resveratrol against hydrogen peroxide (H2O2 )-induced oxidative stress in healthy human and lung cancer cells. Materials and Methods: The cytotoxic doses and IC50 values of resveratrol and hydrogen peroxide for cells were determined by the Cell Titer Blue-Viability Assay kit. The amount of malondialdehyde (MDA) was determined by fluorescence spectrophotometer. The amount of intracellular reduced glutathione level and antioxidant enzyme activities were analyzed by spectrophotometric methods. Results: In both cells, H2O2 treatment alone (IC50 and IC50) increased MDA, glutathione reductase, glutathione S-transferase, selenium-dependent glutathione peroxidase and non-selenium-dependent glutathione peroxidase activities, but glutathione levels decreased compared to the H2O + 2 resveratrol treatment. In addition, high doses of resveratrol alone (IC50 and IC70) induced more oxidative stress in cancer cells than in healthy cells. High doses of resveratrol alone (IC50 and IC70) also showed cytotoxic effects in cells and decreased cell viability. Resveratrol caused more cytotoxic effects in cancer cells compared to healthy cells. Conclusion: The results of this study show that the increase in MDA level and antioxidant enzyme activity caused by highdose resveratrol treatment reveals the prooxidant effect of resveratrol. Our results also showed an antioxidant effect by reducing oxidative stress in cells pre-incubated with low-dose resveratrol and then exposed to H2O2 . Resveratrol has a dose-dependent biphasic (pro/antioxidant) effect on the antioxidant mechanism of cells. However, more research is needed to confirm thishttps://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C49F458445A041BA8E77C8287F678B92antioxidant enzymesresveratroldose-dependent toxicityhydrogen peroxide |
| spellingShingle | Öznur Yurdakul Aysun Özkan Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells European Journal of Biology antioxidant enzymes resveratrol dose-dependent toxicity hydrogen peroxide |
| title | Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells |
| title_full | Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells |
| title_fullStr | Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells |
| title_full_unstemmed | Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells |
| title_short | Resveratrol Dose-Dependently Protects the Antioxidant Mechanism of Hydrogen Peroxide-Exposed Healthy Cells and Lung Cancer Cells |
| title_sort | resveratrol dose dependently protects the antioxidant mechanism of hydrogen peroxide exposed healthy cells and lung cancer cells |
| topic | antioxidant enzymes resveratrol dose-dependent toxicity hydrogen peroxide |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/C49F458445A041BA8E77C8287F678B92 |
| work_keys_str_mv | AT oznuryurdakul resveratroldosedependentlyprotectstheantioxidantmechanismofhydrogenperoxideexposedhealthycellsandlungcancercells AT aysunozkan resveratroldosedependentlyprotectstheantioxidantmechanismofhydrogenperoxideexposedhealthycellsandlungcancercells |